David Bacsik
@dbacsik.bsky.social
Tracing viruses in Perú w/ Dr. Pablo Tsukayama @ UPCH and Dr. Alex Greninger @ UW Virology
Before: Developing single-cell methods for influenza biology w/ @jbloomlab.bsky.social
MD/PhD Student @ University of Washington
Before: Developing single-cell methods for influenza biology w/ @jbloomlab.bsky.social
MD/PhD Student @ University of Washington
8/ The final version of this manuscript is now published in the Journal of Clinical Microbiology. It includes updated results from 150 clinical tests performed through the end of April, none of which detected subtype H5 influenza A virus.
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
Validation of H5 influenza virus subtyping RT-qPCR assay and low prevalence of H5 detection in 2024–2025 influenza virus season | Journal of Clinical Microbiology
The spread of H5N1 influenza virus in the United States has led to the culling of
almost 200 million birds, infected cow herds across 17 states, and resulted in 70
human infections as of July 2025. Ra...
journals.asm.org
October 21, 2025 at 3:44 PM
8/ The final version of this manuscript is now published in the Journal of Clinical Microbiology. It includes updated results from 150 clinical tests performed through the end of April, none of which detected subtype H5 influenza A virus.
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
7/ Clinicians: The H5 subtyping PCR is available to order now through UW Medicine: testguide.labmed.uw.edu/view/FLUH5
Influenza A H5 Subtyping by PCR
testguide.labmed.uw.edu
March 19, 2025 at 2:23 PM
7/ Clinicians: The H5 subtyping PCR is available to order now through UW Medicine: testguide.labmed.uw.edu/view/FLUH5
6/ Read more in our manuscript from the
Greninger Lab
at UW Virology
: medrxiv.org/cgi/content/.... Many people contributed to this work, including
@margaretgracemills.bsky.social, Ailyn Perez-Osorio, and
@pavitrarc.bsky.social.
Greninger Lab
at UW Virology
: medrxiv.org/cgi/content/.... Many people contributed to this work, including
@margaretgracemills.bsky.social, Ailyn Perez-Osorio, and
@pavitrarc.bsky.social.
Validation of H5 influenza virus subtyping RT-qPCR assay and low prevalence of H5 detection in 2024-2025 influenza virus season
A sustained outbreak of H5N1 influenza virus among wild fowl and domestic livestock has caused more than 70 zoonotic infections in humans in the United States, including one death. The Centers for Dis...
medrxiv.org
March 19, 2025 at 2:22 PM
6/ Read more in our manuscript from the
Greninger Lab
at UW Virology
: medrxiv.org/cgi/content/.... Many people contributed to this work, including
@margaretgracemills.bsky.social, Ailyn Perez-Osorio, and
@pavitrarc.bsky.social.
Greninger Lab
at UW Virology
: medrxiv.org/cgi/content/.... Many people contributed to this work, including
@margaretgracemills.bsky.social, Ailyn Perez-Osorio, and
@pavitrarc.bsky.social.
5/ The CDC recommends subtyping all hospitalized flu A+ cases. With H5N1 still circulating widely in birds & cattle, monitoring remains critical—especially for patients with close animal contact, who face the highest risk.
March 19, 2025 at 2:20 PM
5/ The CDC recommends subtyping all hospitalized flu A+ cases. With H5N1 still circulating widely in birds & cattle, monitoring remains critical—especially for patients with close animal contact, who face the highest risk.
4/ This suggests that, in Seattle, the 2024-2025 flu season has been driven by seasonal strains, with no H5N1 detected among this cohort of patients with lab-confirmed influenza A infections.
March 19, 2025 at 2:20 PM
4/ This suggests that, in Seattle, the 2024-2025 flu season has been driven by seasonal strains, with no H5N1 detected among this cohort of patients with lab-confirmed influenza A infections.
3/ So far, none of the samples tested have been H5-positive. All sequenced samples contained seasonal H3N2 or H1N1 viruses.
March 19, 2025 at 2:20 PM
3/ So far, none of the samples tested have been H5-positive. All sequenced samples contained seasonal H3N2 or H1N1 viruses.
2/ In Dr. Alex Greninger’s lab at UW Virology, we developed a clinical RT-qPCR assay for H5 subtyping. We used it to differentiate seasonal influenza A infections from subtype H5 infections during Seattle’s 2024-2025 influenza season.
March 19, 2025 at 2:20 PM
2/ In Dr. Alex Greninger’s lab at UW Virology, we developed a clinical RT-qPCR assay for H5 subtyping. We used it to differentiate seasonal influenza A infections from subtype H5 infections during Seattle’s 2024-2025 influenza season.