👍 Future works should ideally satisfy all the steps outlined to ensure confidence in the metabolites found to be significant for a specific cancer . They should clearly indicate their limitations by specifying the workflow steps completed and those that require further research.

🤔 The analytical laboratories working on MS-based metabolomics for clinical applications should consider that they are providing crucial data for medical researchers, being aware of the need of providing robust results to cancer research.

💡 With the main objective of favouring the standardization, we propose a workflow for the establishment of cancer biomarkers which includes different pivotal steps and issues that must be carefully evaluated for maximizing robustness and reliability on the proposed biomarkers.

📜 One of the key aspects is the need for a consensus in MS-based metabolomics for the discovery of clinical biomarkers and their implementation for the early-diagnosis of cancer.

📝 In this study, we identified the most critical aspects of the metabolomics workflow that should be carefully addressed in future mass spectrometry-based metabolomics studies in cancer diagnosis.

🤝 This thorough and critical review of the MS-based metabolomics studies for cancer biomarker of the last 7 years has been possible thanks to the strong collaboration, passion and dedication of different researchers from Applied #Metabolomics research group and @researchmar.bsky.social.