David
@dav1dcg.bsky.social
All about neuronal activity: development, visual cortex, neuromodulation, interneurons, patch-clamp, and in vivo calcium imaging.
Neuroscience blog: https://spikesandbursts.wordpress.com/
Neuroscience blog: https://spikesandbursts.wordpress.com/
REM sleep reduces subthreshold depolarization in cortical pyramidal neurons in a region-specific manner: doi.org/10.1016/j.ce...
"NMDA receptors drive subthreshold depolarization specifically during REM sleep."
"NMDA receptors drive subthreshold depolarization specifically during REM sleep."
November 9, 2025 at 8:56 PM
REM sleep reduces subthreshold depolarization in cortical pyramidal neurons in a region-specific manner: doi.org/10.1016/j.ce...
"NMDA receptors drive subthreshold depolarization specifically during REM sleep."
"NMDA receptors drive subthreshold depolarization specifically during REM sleep."
Modulation of speed-dependent acetylcholine release in the hippocampus by spatial task engagement: www.cell.com/cell-reports...
October 19, 2025 at 5:59 PM
Modulation of speed-dependent acetylcholine release in the hippocampus by spatial task engagement: www.cell.com/cell-reports...
Distinct representations of economic variables across regions and projections of the frontal cortex: www.cell.com/neuron/fullt...
Task for expected value and risk associated with reward-predicting stimuli. Stronger expected-value coding in dorsal cortex, risk coding in medial cortex.
Task for expected value and risk associated with reward-predicting stimuli. Stronger expected-value coding in dorsal cortex, risk coding in medial cortex.
October 19, 2025 at 5:57 PM
Distinct representations of economic variables across regions and projections of the frontal cortex: www.cell.com/neuron/fullt...
Task for expected value and risk associated with reward-predicting stimuli. Stronger expected-value coding in dorsal cortex, risk coding in medial cortex.
Task for expected value and risk associated with reward-predicting stimuli. Stronger expected-value coding in dorsal cortex, risk coding in medial cortex.
Ultra-high-density Neuropixels probes improve detection and identification in neuronal recordings: www.cell.com/neuron/fullt...
6 μm site-to-site spacing.
Cortical cell types could be discriminated from one another with ∼80% accuracy and from other neurons with ∼85% accuracy.
6 μm site-to-site spacing.
Cortical cell types could be discriminated from one another with ∼80% accuracy and from other neurons with ∼85% accuracy.
October 5, 2025 at 8:15 PM
Ultra-high-density Neuropixels probes improve detection and identification in neuronal recordings: www.cell.com/neuron/fullt...
6 μm site-to-site spacing.
Cortical cell types could be discriminated from one another with ∼80% accuracy and from other neurons with ∼85% accuracy.
6 μm site-to-site spacing.
Cortical cell types could be discriminated from one another with ∼80% accuracy and from other neurons with ∼85% accuracy.
Sensory origin of visually evoked activity in auditory cortex: www.cell.com/cell-reports...
Visual stimuli rarely evoke face movements.
Primary auditory cortex responses to visual input remain robust even in the absence of face movement, and optogenetic silencing of V1 reduces A1 visual responses.
Visual stimuli rarely evoke face movements.
Primary auditory cortex responses to visual input remain robust even in the absence of face movement, and optogenetic silencing of V1 reduces A1 visual responses.
October 5, 2025 at 7:56 PM
Sensory origin of visually evoked activity in auditory cortex: www.cell.com/cell-reports...
Visual stimuli rarely evoke face movements.
Primary auditory cortex responses to visual input remain robust even in the absence of face movement, and optogenetic silencing of V1 reduces A1 visual responses.
Visual stimuli rarely evoke face movements.
Primary auditory cortex responses to visual input remain robust even in the absence of face movement, and optogenetic silencing of V1 reduces A1 visual responses.
A real-time all-optical interface for dynamic perturbation of neural activity during behavior: www.sciencedirect.com/science/arti...
Real-time interface that integrates CaImAn for online calcium imaging analysis, and a custom hologram program for two-photon optogenetic stimulation.
Real-time interface that integrates CaImAn for online calcium imaging analysis, and a custom hologram program for two-photon optogenetic stimulation.
September 28, 2025 at 7:42 PM
A real-time all-optical interface for dynamic perturbation of neural activity during behavior: www.sciencedirect.com/science/arti...
Real-time interface that integrates CaImAn for online calcium imaging analysis, and a custom hologram program for two-photon optogenetic stimulation.
Real-time interface that integrates CaImAn for online calcium imaging analysis, and a custom hologram program for two-photon optogenetic stimulation.
Cooperative actions of interneuron families support the hippocampal spatial code: science.org/doi/10.1126/...
In vivo optogenetics of interneurons + ephys recordings shows tIme division control of place fields of pyramidal cells: PV first half, Sst and Id2 the second half.
In vivo optogenetics of interneurons + ephys recordings shows tIme division control of place fields of pyramidal cells: PV first half, Sst and Id2 the second half.
September 7, 2025 at 7:54 PM
Cooperative actions of interneuron families support the hippocampal spatial code: science.org/doi/10.1126/...
In vivo optogenetics of interneurons + ephys recordings shows tIme division control of place fields of pyramidal cells: PV first half, Sst and Id2 the second half.
In vivo optogenetics of interneurons + ephys recordings shows tIme division control of place fields of pyramidal cells: PV first half, Sst and Id2 the second half.
High-speed voltage imaging of action potentials in molecular layer interneurons reveals sensory-driven synchrony that augments movement: www.cell.com/cell-reports...
Voltage imaging of fast-spiking interneurons in the cerebellum with 1-photon imaging at 2-4 kHz.
Voltage imaging of fast-spiking interneurons in the cerebellum with 1-photon imaging at 2-4 kHz.
August 17, 2025 at 6:33 PM
High-speed voltage imaging of action potentials in molecular layer interneurons reveals sensory-driven synchrony that augments movement: www.cell.com/cell-reports...
Voltage imaging of fast-spiking interneurons in the cerebellum with 1-photon imaging at 2-4 kHz.
Voltage imaging of fast-spiking interneurons in the cerebellum with 1-photon imaging at 2-4 kHz.
Cambrian origin of the arachnid brain: www.cell.com/current-biol...
Reconstruction of fossil neural traces in Cambrian chelicerates shows similarities to the brains of living spiders and scorpions.
Cool and interesting!
Reconstruction of fossil neural traces in Cambrian chelicerates shows similarities to the brains of living spiders and scorpions.
Cool and interesting!
July 29, 2025 at 5:09 PM
Cambrian origin of the arachnid brain: www.cell.com/current-biol...
Reconstruction of fossil neural traces in Cambrian chelicerates shows similarities to the brains of living spiders and scorpions.
Cool and interesting!
Reconstruction of fossil neural traces in Cambrian chelicerates shows similarities to the brains of living spiders and scorpions.
Cool and interesting!
A cell type in the visual system that receives feedback about limb movement: doi.org/10.1016/j.cu...
LT52 neurons in Drosophila visual system are strongly activated when flies groom their heads and driven by a combination of visual and non-visual inputs.
LT52 neurons in Drosophila visual system are strongly activated when flies groom their heads and driven by a combination of visual and non-visual inputs.
July 20, 2025 at 5:45 PM
A cell type in the visual system that receives feedback about limb movement: doi.org/10.1016/j.cu...
LT52 neurons in Drosophila visual system are strongly activated when flies groom their heads and driven by a combination of visual and non-visual inputs.
LT52 neurons in Drosophila visual system are strongly activated when flies groom their heads and driven by a combination of visual and non-visual inputs.
Activity-dependent development of synaptic circuits mediates direction selectivity in an axis-specific manner: doi.org/10.1016/j.ce...
"before the onset of vision, spontaneous activity selectively influences the formation of precise wiring essential for motion detection along the horizontal axis."
"before the onset of vision, spontaneous activity selectively influences the formation of precise wiring essential for motion detection along the horizontal axis."
June 29, 2025 at 7:26 PM
Activity-dependent development of synaptic circuits mediates direction selectivity in an axis-specific manner: doi.org/10.1016/j.ce...
"before the onset of vision, spontaneous activity selectively influences the formation of precise wiring essential for motion detection along the horizontal axis."
"before the onset of vision, spontaneous activity selectively influences the formation of precise wiring essential for motion detection along the horizontal axis."
Is criticality a unified setpoint of brain function?: www.cell.com/neuron/fullt...
"We perform a meta-analysis of 140 datasets published between 2003 and 2024. We find that a long-standing controversy is the product of a methodological choice with no bearing on underlying dynamics."
"We perform a meta-analysis of 140 datasets published between 2003 and 2024. We find that a long-standing controversy is the product of a methodological choice with no bearing on underlying dynamics."
June 29, 2025 at 7:22 PM
Is criticality a unified setpoint of brain function?: www.cell.com/neuron/fullt...
"We perform a meta-analysis of 140 datasets published between 2003 and 2024. We find that a long-standing controversy is the product of a methodological choice with no bearing on underlying dynamics."
"We perform a meta-analysis of 140 datasets published between 2003 and 2024. We find that a long-standing controversy is the product of a methodological choice with no bearing on underlying dynamics."
Three-photon microscopy: an emerging technique for deep intravital brain imaging: www.nature.com/articles/s41...
Review of the principles and applications of three-photon (3P) microscopy.
Review of the principles and applications of three-photon (3P) microscopy.
June 29, 2025 at 7:18 PM
Three-photon microscopy: an emerging technique for deep intravital brain imaging: www.nature.com/articles/s41...
Review of the principles and applications of three-photon (3P) microscopy.
Review of the principles and applications of three-photon (3P) microscopy.
A reduced ability to discriminate social from non-social touch at the circuit level may underlie social avoidance in autism: nature.com/articles/s41...
Results suggest that "a reduced capacity to represent social stimuli at the circuit level may underlie social avoidance in autism."
Results suggest that "a reduced capacity to represent social stimuli at the circuit level may underlie social avoidance in autism."
May 18, 2025 at 7:58 PM
A reduced ability to discriminate social from non-social touch at the circuit level may underlie social avoidance in autism: nature.com/articles/s41...
Results suggest that "a reduced capacity to represent social stimuli at the circuit level may underlie social avoidance in autism."
Results suggest that "a reduced capacity to represent social stimuli at the circuit level may underlie social avoidance in autism."
Modulating cortical excitability and cortical arousal by pupil self-regulation: nature.com/articles/s41...
Pupil self-regulation modulated electrophysiological markers of cortical excitability, cortical arousal, and stimulus evaluation.
Pupil self-regulation modulated electrophysiological markers of cortical excitability, cortical arousal, and stimulus evaluation.
May 18, 2025 at 7:57 PM
Modulating cortical excitability and cortical arousal by pupil self-regulation: nature.com/articles/s41...
Pupil self-regulation modulated electrophysiological markers of cortical excitability, cortical arousal, and stimulus evaluation.
Pupil self-regulation modulated electrophysiological markers of cortical excitability, cortical arousal, and stimulus evaluation.
Network influence determines the impact of cortical ensembles on stimulus detection: cell.com/neuron/fullt...
"Mice detect stimuli by reading out all V1 activity, not by weighting key neurons"
"Mice detect stimuli by reading out all V1 activity, not by weighting key neurons"
May 18, 2025 at 7:57 PM
Network influence determines the impact of cortical ensembles on stimulus detection: cell.com/neuron/fullt...
"Mice detect stimuli by reading out all V1 activity, not by weighting key neurons"
"Mice detect stimuli by reading out all V1 activity, not by weighting key neurons"
Motor and vestibular signals in the visual cortex permit the separation of self versus externally generated visual motion: doi.org/10.1016/j.ce...
Cells in the primary visual cortex receive running, translation, and visual motion signals to accurately process visual flow information.
Cells in the primary visual cortex receive running, translation, and visual motion signals to accurately process visual flow information.
April 27, 2025 at 7:48 PM
Motor and vestibular signals in the visual cortex permit the separation of self versus externally generated visual motion: doi.org/10.1016/j.ce...
Cells in the primary visual cortex receive running, translation, and visual motion signals to accurately process visual flow information.
Cells in the primary visual cortex receive running, translation, and visual motion signals to accurately process visual flow information.
Far-red fluorescent genetically encoded calcium ion indicators: www.nature.com/articles/s41...
New two far-red fluorescent GECIs (calcium indicators):
- FR-GECO1a: λex max=596 nm, ΔF/F0 (in vitro)=6, Kd=29 nM
- FR-GECO1c: λex max=644 nm, ΔF/F0 (in vitro)=18, Kd=83 nM
New two far-red fluorescent GECIs (calcium indicators):
- FR-GECO1a: λex max=596 nm, ΔF/F0 (in vitro)=6, Kd=29 nM
- FR-GECO1c: λex max=644 nm, ΔF/F0 (in vitro)=18, Kd=83 nM
April 13, 2025 at 5:59 PM
Far-red fluorescent genetically encoded calcium ion indicators: www.nature.com/articles/s41...
New two far-red fluorescent GECIs (calcium indicators):
- FR-GECO1a: λex max=596 nm, ΔF/F0 (in vitro)=6, Kd=29 nM
- FR-GECO1c: λex max=644 nm, ΔF/F0 (in vitro)=18, Kd=83 nM
New two far-red fluorescent GECIs (calcium indicators):
- FR-GECO1a: λex max=596 nm, ΔF/F0 (in vitro)=6, Kd=29 nM
- FR-GECO1c: λex max=644 nm, ΔF/F0 (in vitro)=18, Kd=83 nM
Quantification of the effect of hemodynamic occlusion in two-photon imaging: elifesciences.org/reviewed-pre...
Quantification of hemodynamic occlusion effects in WF and 2P using GFP expression revealed signal changes that were often comparable to those of activity sensors.
Quantification of hemodynamic occlusion effects in WF and 2P using GFP expression revealed signal changes that were often comparable to those of activity sensors.
April 6, 2025 at 7:38 PM
Quantification of the effect of hemodynamic occlusion in two-photon imaging: elifesciences.org/reviewed-pre...
Quantification of hemodynamic occlusion effects in WF and 2P using GFP expression revealed signal changes that were often comparable to those of activity sensors.
Quantification of hemodynamic occlusion effects in WF and 2P using GFP expression revealed signal changes that were often comparable to those of activity sensors.
Neural correlates of visual object recognition in rats: www.cell.com/cell-reports...
Nice study on the visual cortex of rats.
Nice study on the visual cortex of rats.
March 30, 2025 at 6:14 PM
Neural correlates of visual object recognition in rats: www.cell.com/cell-reports...
Nice study on the visual cortex of rats.
Nice study on the visual cortex of rats.
Silent Synapses in the Adult Brain
www.annualreviews.org/content/jour...
"review current evidence for silent synapses in the adult brain and explore the potential roles of these highly plastic structures."
www.annualreviews.org/content/jour...
"review current evidence for silent synapses in the adult brain and explore the potential roles of these highly plastic structures."
March 2, 2025 at 6:27 PM
Silent Synapses in the Adult Brain
www.annualreviews.org/content/jour...
"review current evidence for silent synapses in the adult brain and explore the potential roles of these highly plastic structures."
www.annualreviews.org/content/jour...
"review current evidence for silent synapses in the adult brain and explore the potential roles of these highly plastic structures."
And the 8-pixel version, just for fun.
February 27, 2025 at 6:49 PM
And the 8-pixel version, just for fun.
Sleep-wake-related changes in intracellular chloride regulate plasticity at glutamatergic cortical synapses: doi.org/10.1016/j.cu...
Sleep-Wake-Related changes in Cl-dependent reversal potential of GABAA Receptors predict and regulate glutamatergic LTP in cortical PYR neurons
Sleep-Wake-Related changes in Cl-dependent reversal potential of GABAA Receptors predict and regulate glutamatergic LTP in cortical PYR neurons
February 23, 2025 at 8:19 PM
Sleep-wake-related changes in intracellular chloride regulate plasticity at glutamatergic cortical synapses: doi.org/10.1016/j.cu...
Sleep-Wake-Related changes in Cl-dependent reversal potential of GABAA Receptors predict and regulate glutamatergic LTP in cortical PYR neurons
Sleep-Wake-Related changes in Cl-dependent reversal potential of GABAA Receptors predict and regulate glutamatergic LTP in cortical PYR neurons
Binocular processing facilitates escape behavior through multiple pathways to the superior colliculus: www.cell.com/current-biol...
SC superficial layers receive visual info via retinal input, whereas intermediate and deep layers rely on interhemispheric and corticotectal pathways
SC superficial layers receive visual info via retinal input, whereas intermediate and deep layers rely on interhemispheric and corticotectal pathways
February 23, 2025 at 8:13 PM
Binocular processing facilitates escape behavior through multiple pathways to the superior colliculus: www.cell.com/current-biol...
SC superficial layers receive visual info via retinal input, whereas intermediate and deep layers rely on interhemispheric and corticotectal pathways
SC superficial layers receive visual info via retinal input, whereas intermediate and deep layers rely on interhemispheric and corticotectal pathways
A neural basis for prosocial behavior toward unresponsive individuals: www.science.org/doi/10.1126/...
February 23, 2025 at 8:07 PM
A neural basis for prosocial behavior toward unresponsive individuals: www.science.org/doi/10.1126/...