@cyanometals
@cyanometals.bsky.social
Interested in metals and cyanobacteria, in any order. Based @IBVF_Sevilla, Universidad de Sevilla/CSIC. Views my own.
https://www.ibvf.us-csic.es/grupos-investigacion/stress-response-and-synthetic-biology-of-photosynthetic-microorganisms/?lang=en
https://www.ibvf.us-csic.es/grupos-investigacion/stress-response-and-synthetic-biology-of-photosynthetic-microorganisms/?lang=en
It works ok in cyanobacteria bit decreases a bit frequency of recombination whem compared to the same total homology (750bp each side)
June 3, 2025 at 6:41 AM
It works ok in cyanobacteria bit decreases a bit frequency of recombination whem compared to the same total homology (750bp each side)
It is expressed constitutively? For plastocyanin (which is also a blue copper protein) copper is added once you have biomass and induce expression of the protein for better yields
March 11, 2025 at 4:46 PM
It is expressed constitutively? For plastocyanin (which is also a blue copper protein) copper is added once you have biomass and induce expression of the protein for better yields
did the one with copper grow?
March 11, 2025 at 4:32 PM
did the one with copper grow?
azurin is probably periplasmic. have you clone the leader peptide? it might be better to add copper once you have some biomass
March 11, 2025 at 4:26 PM
azurin is probably periplasmic. have you clone the leader peptide? it might be better to add copper once you have some biomass
1mM migth be too high but you have to try
March 11, 2025 at 6:33 AM
1mM migth be too high but you have to try