Viny Lab
@cohesinlab.bsky.social
Summary
We identify Stag2 as a key enforcer of erythroid identity through its role in shaping the chromatin landscape that guides Gata1 binding. This underscores the importance of coordinated transcription factor and chromatin interactions in lineage specification.
We identify Stag2 as a key enforcer of erythroid identity through its role in shaping the chromatin landscape that guides Gata1 binding. This underscores the importance of coordinated transcription factor and chromatin interactions in lineage specification.
September 22, 2025 at 2:29 PM
Summary
We identify Stag2 as a key enforcer of erythroid identity through its role in shaping the chromatin landscape that guides Gata1 binding. This underscores the importance of coordinated transcription factor and chromatin interactions in lineage specification.
We identify Stag2 as a key enforcer of erythroid identity through its role in shaping the chromatin landscape that guides Gata1 binding. This underscores the importance of coordinated transcription factor and chromatin interactions in lineage specification.
Human Relevance
We extended findings to human CD34⁺ cells & MDS patients:
- STAG2 loss impaired erythroid differentiation
- Altered GATA1 target gene expression
🧍♂️➡️🧬⬅️🐭
We extended findings to human CD34⁺ cells & MDS patients:
- STAG2 loss impaired erythroid differentiation
- Altered GATA1 target gene expression
🧍♂️➡️🧬⬅️🐭
September 22, 2025 at 2:29 PM
Human Relevance
We extended findings to human CD34⁺ cells & MDS patients:
- STAG2 loss impaired erythroid differentiation
- Altered GATA1 target gene expression
🧍♂️➡️🧬⬅️🐭
We extended findings to human CD34⁺ cells & MDS patients:
- STAG2 loss impaired erythroid differentiation
- Altered GATA1 target gene expression
🧍♂️➡️🧬⬅️🐭
Functional Validation
Functionally, we observed that Stag2 KO EryPs:
- Produced fewer erythroid cells
- Generated more megakaryocytes
Functionally, we observed that Stag2 KO EryPs:
- Produced fewer erythroid cells
- Generated more megakaryocytes
September 22, 2025 at 2:29 PM
Functional Validation
Functionally, we observed that Stag2 KO EryPs:
- Produced fewer erythroid cells
- Generated more megakaryocytes
Functionally, we observed that Stag2 KO EryPs:
- Produced fewer erythroid cells
- Generated more megakaryocytes
Multi-omic Insight
RNA-seq, ATAC-seq, and GATA1 CUT&RUN of EryPs revealed:
- ↓ accessibility & GATA1 binding at erythroid genes
- ↑ accessibility & GATA1 binding at megakaryocyte genes
Stag2 loss rewires the GATA1 cistrome.
RNA-seq, ATAC-seq, and GATA1 CUT&RUN of EryPs revealed:
- ↓ accessibility & GATA1 binding at erythroid genes
- ↑ accessibility & GATA1 binding at megakaryocyte genes
Stag2 loss rewires the GATA1 cistrome.
September 22, 2025 at 2:29 PM
Multi-omic Insight
RNA-seq, ATAC-seq, and GATA1 CUT&RUN of EryPs revealed:
- ↓ accessibility & GATA1 binding at erythroid genes
- ↑ accessibility & GATA1 binding at megakaryocyte genes
Stag2 loss rewires the GATA1 cistrome.
RNA-seq, ATAC-seq, and GATA1 CUT&RUN of EryPs revealed:
- ↓ accessibility & GATA1 binding at erythroid genes
- ↑ accessibility & GATA1 binding at megakaryocyte genes
Stag2 loss rewires the GATA1 cistrome.
GATA1 is a master regulator of 🩸, yet it has pleiotropic functions, particularly in erythroid/megakaryocytic ontogeny. Inspired by the open Qs in John Crispino's review (PMID: 15659348) we wanted to understand how GATA1 enforces lineage specificity across divergent fates.
September 22, 2025 at 2:29 PM
GATA1 is a master regulator of 🩸, yet it has pleiotropic functions, particularly in erythroid/megakaryocytic ontogeny. Inspired by the open Qs in John Crispino's review (PMID: 15659348) we wanted to understand how GATA1 enforces lineage specificity across divergent fates.