But in general, the hsp70 gene was a convenient cloned source for promoters and terminators for making constructs back in the early 90s, and its possible that some the temp sensitive genetic components unwittingly were included in GAL4/UAS constructs.

It's also possible that the GAL4 protein, since it comes from yeast, is optimized in structure for yeast growth temperatures, which are closer to 32-34C. So the protein is 'happier' at higher temperatures. Given how strongly we express GAL4, I dont think we'd be able to see such effects.

It's a great question! The most likely culprit is the use of the heatshock70 terminator in the design of the GAL4 enhancer trap construct and the hsp70 promoter in the UASt vector. Its possible some temp sensitive elements might still be present.

Gal4 itself is not temperature sensitive. We tested this in our paper (Riabinina, Nature Methods, 2015). Additional refs to this fact also in that paper.
That being said, many GAL4 lines are temp sensitive, which likely reflects the use of temperature sensitive components.

Probably just The Constitution.

Congrats!

Our experiences over the past 3 years suggest it may be valuable to understand these attacks on science as extensions of the Big Lie (of election fraud), Covid denialism, & Jim Crow 2.0 — part of a collective effort to bend reality, science & values to align with the central fantasies of Trumpism.

This is fantastic!

Great job! Your parents must have been so proud!
I really hope you get your R01 grant funding. The research sounds fascinating and important.

Proposed new term for members of the Trump administration committing and supporting this behavior

CICADAS

Cowardly Information, Content, And Data Analysis Suppressors