Happy to finally publish this work… Congrats authors and thanks funders @agencerecherche.bsky.social #FondationBettencourtSchueller‬‬‬

These findings help to establish THG microscopy as a quantitative, multiscale imaging technique, paving the way for new applications. 📖 #Neuroscience #Myelin #THG #Microscopy #Research

Fun fact for multiphoton microscopy geeks: aberrations caused by vertical micro-interfaces appear to be a significant contrast mechanism in polarized THG imaging. 😎

Finally, we illustrate the relevance of pTHG microscopy for characterizing myelin distribution at different scales in fixed mouse and human brain tissue.

Our study presents a systematic characterization of polarized THG (pTHG) signals from individual axons, enabling the derivation of myelin scores in various systems. 🐭🐟
We confirm the sensitivity of THG for myelin in mouse tissue and detect early axon development in zebrafish.

We show that axons exhibit complex pTHG profiles that depend on axon morphology and can't be explained by classical models. We establish a numerical strategy to relate pTHG profiles to axon diameter and myelin thickness, taking into account myelin-induced optical aberrations.

By #J_Morizet #N_Olivier #MS_Aigrot #P_Mahou #E_Martin #A_Desmazieres #B_Stankoff @chiarastringari.bsky.social

🔬🌟 Find out how Third Harmonic Generation (THG) microscopy could help advance myelin biomedical research! 🧠