Carlos Guardia
@charlyguardia.bsky.social
NewPI@NIEHS | Stadtman TTI | NIH DSP #2021 | Cell Biology - Autophagy - Intracellular Traffic - Placenta | My opinions are my own | 🇦🇷🏳️🌈🇺🇸
We hope next to dig deeper into the mechanistic aspects that can fully explain this phenotype, expand on early (development) and late (cancer, metabolic disease) trajectories, and interrogate key environmental stressors that could potentiate or inhibit the disruption in tissue-specific autophagy.
September 3, 2025 at 4:56 PM
We hope next to dig deeper into the mechanistic aspects that can fully explain this phenotype, expand on early (development) and late (cancer, metabolic disease) trajectories, and interrogate key environmental stressors that could potentiate or inhibit the disruption in tissue-specific autophagy.
Now, we have confirmed this phenotype in mice, demonstrated fragmentation of mitochondria, ↑LC3B-II
& SQSTM1↑ puncta (incl. short isoforms) and Nrf2–NQO1 detox pathway upregulation. This led to hepatomegaly with disrupted cellular architecture but no signs of early cancer as seen in other ATG KOs.
& SQSTM1↑ puncta (incl. short isoforms) and Nrf2–NQO1 detox pathway upregulation. This led to hepatomegaly with disrupted cellular architecture but no signs of early cancer as seen in other ATG KOs.
September 3, 2025 at 4:56 PM
Now, we have confirmed this phenotype in mice, demonstrated fragmentation of mitochondria, ↑LC3B-II
& SQSTM1↑ puncta (incl. short isoforms) and Nrf2–NQO1 detox pathway upregulation. This led to hepatomegaly with disrupted cellular architecture but no signs of early cancer as seen in other ATG KOs.
& SQSTM1↑ puncta (incl. short isoforms) and Nrf2–NQO1 detox pathway upregulation. This led to hepatomegaly with disrupted cellular architecture but no signs of early cancer as seen in other ATG KOs.
This story is a continuation of the work Elodie and I started while at @juanbonifacino.bsky.social lab where we observed lipid droplets accumulation in ATG9A KO cells and Atg9 KO worms: www.nature.com/articles/s41...
The autophagy protein ATG9A enables lipid mobilization from lipid droplets - Nature Communications
ATG9A is transmembrane autophagic machinery protein that delivers phospholipids to expanding autophagosomes. Mailler et al. show that ATG9A is required to mobilize lipids from lipid droplets for autop...
www.nature.com
September 3, 2025 at 4:56 PM
This story is a continuation of the work Elodie and I started while at @juanbonifacino.bsky.social lab where we observed lipid droplets accumulation in ATG9A KO cells and Atg9 KO worms: www.nature.com/articles/s41...