Plant Science Research Weekly: July 18, 2025 plantae.org/plant-scienc... (3/3) The hidden power of water in plant regeneration; ; Unusual cell death pathway in maize endosperm; Herbivore bites promote plant growth in the succeeding year. @matthiaserb.bsky.social @charlesmelnyk.bsky.social

Interesting! Probably a piece of the Che was left on and grew back. Much less likely but maybe something chimeric or a hybrid? Chimeras have been seen in orange before (the Bizzarria). Have a look at these reviews:
onlinelibrary.wiley.com/doi/10.1111/...
www.sciencedirect.com/science/arti...

9/n. A collaborative effort with Abdul Kareem, Anna van Wüllen, Ai Zhang, Gabriel Walckiers and Ellen Fasth. A huge thanks to our funders @erc.europa.eu, @kawresearch.bsky.social and @vetenskapsradet.bsky.social for supporting and enabling this research!

8/n. An interesting outcome of this work is that high water availability is really good at regenerating roots and promoting in vitro regeneration (pubmed.ncbi.nlm.nih.gov/20230752). If you want to improve your in vitro regeneration rates, try using lower agar concentrations in your media.

7/n. What’s the relevance of this? We used air layering (www.rhs.org.uk/propagation/...) in tomato and found wet soil induced roots but dry soil induced callus. Thus, when wounds are in contact with water, roots form (think of plant propagation!) whereas under dry conditions, callus seals the wound

6/n. High water promoted ethylene and jasmonic acid responses, and these hormones could shift the auxin maxima away from the wound likely via PIN transporters. Thus, high water induced ethylene and jasmonic acid to change PIN localisation and move auxin maxima away from the wound to promote rooting

5/n. We found that water moved the auxin response maxima at the wound either away (distal) or towards (proximal) the cut. Distal response was associated with root formation while proximal response with callus formation. So the location of auxin response could induce different regeneration fates.

4/n. What decides whether a wound forms roots or callus? Water! By changing water availability, we could transition between these two fates. High water promoted roots near the wound, whereas low water promoted callus formation at the wound. These processes seemed to antagonise one another

3/n. Roots formed at wounds follow a lateral root formation pathway (pubmed.ncbi.nlm.nih.gov/24642937) but what about callus from wounds? We used reporters and mutants to find that callus follows a procambium pathway suggesting that different regeneration fates used different molecular pathways.

2/n. Regeneration is fundamental for plant survival but how do plants know what to regenerate? We sought to address this question using cut Arabidopsis petioles that can either form roots, callus or both at the site of wounding

Thanks Thomas and Hannes for highlighting our work. Great commentary and very well written!

Thanks Suayb! 🙏