Jeff Calhoun
@calhoujd.bsky.social
Research Assistant Professor with Gemma Carvill (@CarvillLab on Twitter). Focus: epilepsy genetics. Twin. Ace cat dad. Occasional writer. I am only an egg. He/him.
this is Zig, my special guy. he was really talkative and loved going for walks around the neighborhood.
November 13, 2025 at 9:50 PM
this is Zig, my special guy. he was really talkative and loved going for walks around the neighborhood.
Couldn't help myself, one last bit to show off both versions of our cliPE logo which brings me joy
January 10, 2025 at 6:49 PM
Couldn't help myself, one last bit to show off both versions of our cliPE logo which brings me joy
Targeted amplicon sequencing by Illumina short reads is used to generate allele frequencies in selected and unselected cell pools, followed by enrichment analysis. 7/11
January 10, 2025 at 6:49 PM
Targeted amplicon sequencing by Illumina short reads is used to generate allele frequencies in selected and unselected cell pools, followed by enrichment analysis. 7/11
After transfection of haploid cells with epegRNA libraries, cells are selected based on gene function. This is highly context-dependent based on the gene of interest. 5/11
January 10, 2025 at 6:49 PM
After transfection of haploid cells with epegRNA libraries, cells are selected based on gene function. This is highly context-dependent based on the gene of interest. 5/11
Archetypal epegRNAs representative of the designed libraries are then screened for editing efficiency. Oligo pools are used to quickly assemble epegRNA libraries 4/11
January 10, 2025 at 6:49 PM
Archetypal epegRNAs representative of the designed libraries are then screened for editing efficiency. Oligo pools are used to quickly assemble epegRNA libraries 4/11
How does cliPE work? Prime editing libraries are designed using the pegRNA Designer Shiny app (developed by talented MSTP student Nico Bodkin!) which quickly designs everything you need for scalable prime editing 3/11
January 10, 2025 at 6:49 PM
How does cliPE work? Prime editing libraries are designed using the pegRNA Designer Shiny app (developed by talented MSTP student Nico Bodkin!) which quickly designs everything you need for scalable prime editing 3/11
#BookChallenge 8/20 (for some of these it's hard not to comment, might need to revisit to add context)
December 18, 2024 at 1:48 PM
#BookChallenge 8/20 (for some of these it's hard not to comment, might need to revisit to add context)
so much this. my Vera won’t let me pick her up but she now naps with either her head or one paw on my leg and its such a win every time 🥹
April 29, 2024 at 10:27 PM
so much this. my Vera won’t let me pick her up but she now naps with either her head or one paw on my leg and its such a win every time 🥹
