@builab.bsky.social
We also analysed SPEF1-KO cilia by cryo-ET to show that SPEF1 restricts C1 and C2 microtubule movement.
July 11, 2025 at 7:41 PM
We also analysed SPEF1-KO cilia by cryo-ET to show that SPEF1 restricts C1 and C2 microtubule movement.
In the final version, we included a lot more data and analysis compared to the previous biorxiv version. We achieved a 4.6 Angstrom resolution of the microtubule by subtomogram averaging of MT-SPEF1 complexes.
July 11, 2025 at 7:41 PM
In the final version, we included a lot more data and analysis compared to the previous biorxiv version. We achieved a 4.6 Angstrom resolution of the microtubule by subtomogram averaging of MT-SPEF1 complexes.
Proudly present our new preprint on my first post here. We performed SPA on the ciliary tip and identify 7 proteins. Among them, we found the conserved protein SPEF1 at the microtubule seam. In vitro assays show that SPEF1 binds to the seam and stablizes microtubules.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
December 4, 2024 at 5:05 PM
Proudly present our new preprint on my first post here. We performed SPA on the ciliary tip and identify 7 proteins. Among them, we found the conserved protein SPEF1 at the microtubule seam. In vitro assays show that SPEF1 binds to the seam and stablizes microtubules.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
The Bui lab Twitter will stop and we are migrating to Mastodon. Anyway, the last post is to thank the @Beck_Laboratory and @jankosinski @aobarska for the new wall art decoration. NPC rules :
December 4, 2024 at 3:55 AM
The Bui lab Twitter will stop and we are migrating to Mastodon. Anyway, the last post is to thank the @Beck_Laboratory and @jankosinski @aobarska for the new wall art decoration. NPC rules :
Taking forever but finally here. We identified close to 40 MIPs in Tetrahymena doublet. Specifically, the MIPs at the outer junction (CFAP77, OJ2 & OJ3). @dynein_awk @TheRealCSBlack @ahmad__khalifa
December 4, 2024 at 3:54 AM
Taking forever but finally here. We identified close to 40 MIPs in Tetrahymena doublet. Specifically, the MIPs at the outer junction (CFAP77, OJ2 & OJ3). @dynein_awk @TheRealCSBlack @ahmad__khalifa
My first contribution to #TeamTomo . Script to help visualize the subtomogram orientations from #Relion star file inside its tomogram using ChimeraX. Thanks to @AlisterBurt for eulerangles & starfile module.
github.com/builab/subtomo…
github.com/builab/subtomo…
December 4, 2024 at 3:54 AM
My first contribution to #TeamTomo . Script to help visualize the subtomogram orientations from #Relion star file inside its tomogram using ChimeraX. Thanks to @AlisterBurt for eulerangles & starfile module.
github.com/builab/subtomo…
github.com/builab/subtomo…
December 4, 2024 at 3:53 AM
Gotta start them young !!! Showed her a cytoskeleton animation from Youtube and introduce her to microtubule manual picking.
December 4, 2024 at 3:53 AM
Gotta start them young !!! Showed her a cytoskeleton animation from Youtube and introduce her to microtubule manual picking.
The cilia mask for our lab has arrived.
December 4, 2024 at 3:53 AM
The cilia mask for our lab has arrived.
Our newest work on the structure and activation of outer arm dyneins in Tetrahymena@Yoshi__Ichikawa @TheRealCSBlack and many twitterless authors
biorxiv.org/content/10.110…
biorxiv.org/content/10.110…
December 4, 2024 at 3:53 AM
Our newest work on the structure and activation of outer arm dyneins in Tetrahymena@Yoshi__Ichikawa @TheRealCSBlack and many twitterless authors
biorxiv.org/content/10.110…
biorxiv.org/content/10.110…
December 4, 2024 at 3:53 AM
Finally, our paper on the proteomics of the central pairs of Chlamydomonas is out. This is an Honour research project turning into a Master Project from Daniel Dai, a student in the lab supervised by my former postdoc @Yoshi__Ichikawa
jstage.jst.go.jp/article/biophy…
jstage.jst.go.jp/article/biophy…
December 4, 2024 at 3:52 AM
Finally, our paper on the proteomics of the central pairs of Chlamydomonas is out. This is an Honour research project turning into a Master Project from Daniel Dai, a student in the lab supervised by my former postdoc @Yoshi__Ichikawa
jstage.jst.go.jp/article/biophy…
jstage.jst.go.jp/article/biophy…
ChimeraX helps us handles so many chains with the doublet microtubule #WeNeedChimeraX
December 4, 2024 at 3:52 AM
ChimeraX helps us handles so many chains with the doublet microtubule #WeNeedChimeraX
Ramping-up research from McGill. Our building Strathcona is built for pandemic ready :
December 4, 2024 at 3:52 AM
Ramping-up research from McGill. Our building Strathcona is built for pandemic ready :
I wanted to buy a poster of patent art of Transmission EM for home decoration but couldn't find a good one. When #StayAtHome, I did it myself. The bigger images are in the following tweets.
December 4, 2024 at 3:52 AM
I wanted to buy a poster of patent art of Transmission EM for home decoration but couldn't find a good one. When #StayAtHome, I did it myself. The bigger images are in the following tweets.
Perfect weather for #SocialDistancing . Snowing in Mid-April.
December 4, 2024 at 3:52 AM
Perfect weather for #SocialDistancing . Snowing in Mid-April.
Miss the sound of preparing your liquid ethane for #cryoEM, I got a solution. #WorkFromHome
December 4, 2024 at 3:51 AM
Miss the sound of preparing your liquid ethane for #cryoEM, I got a solution. #WorkFromHome
We have a lot of funs with the lessons with activities and mini-lessons from. The kids really enjoy some thinkings and doing some sciences. All the lessons are now available until June.
mysteryscience.com
mysteryscience.com
December 4, 2024 at 3:51 AM
We have a lot of funs with the lessons with activities and mini-lessons from. The kids really enjoy some thinkings and doing some sciences. All the lessons are now available until June.
mysteryscience.com
mysteryscience.com
My temp white board for online teaching and online discussion with grad students.
December 4, 2024 at 3:51 AM
My temp white board for online teaching and online discussion with grad students.
The final version of the inner junction paper is out now on @eLife . The whole proof is lightning fast.
elifesciences.org/articles/52760
elifesciences.org/articles/52760
December 4, 2024 at 3:51 AM
The final version of the inner junction paper is out now on @eLife . The whole proof is lightning fast.
elifesciences.org/articles/52760
elifesciences.org/articles/52760
We have a preferred orientation problem in our dessert competition at the @McGill_ACB Xmas party
December 4, 2024 at 3:50 AM
We have a preferred orientation problem in our dessert competition at the @McGill_ACB Xmas party
Reunion with my former postdoc @Yoshi__Ichikawa and former visiting student Shintaroh in Kyoto.
December 4, 2024 at 3:50 AM
Reunion with my former postdoc @Yoshi__Ichikawa and former visiting student Shintaroh in Kyoto.
