Thank youuuu Pingtao
September 5, 2025 at 9:38 AM
Thank youuuu Pingtao
Thank you Tatsuya!!
September 5, 2025 at 9:37 AM
Thank you Tatsuya!!
I am very grateful to Michele & Marc, Takehiro & Dohmae-san, Markus, @yasukadota.bsky.social & @shirasulab.bsky.social for their support. Also big thanks to the reviewers and editors for improving our manuscript!
September 4, 2025 at 7:33 PM
I am very grateful to Michele & Marc, Takehiro & Dohmae-san, Markus, @yasukadota.bsky.social & @shirasulab.bsky.social for their support. Also big thanks to the reviewers and editors for improving our manuscript!
With this approach, we aim to characterize more PRRs against different pathogens & pests in the future.
September 4, 2025 at 7:33 PM
With this approach, we aim to characterize more PRRs against different pathogens & pests in the future.
To summarize, we reported the strategies to discover and engineer plant immune receptors.
As usual, we have uploaded the data, including RCM analysis, receptor & peptide sequences, raw data…etc etc on Zenodo
(zenodo.org/records/1546... )
As usual, we have uploaded the data, including RCM analysis, receptor & peptide sequences, raw data…etc etc on Zenodo
(zenodo.org/records/1546... )
September 4, 2025 at 7:33 PM
To summarize, we reported the strategies to discover and engineer plant immune receptors.
As usual, we have uploaded the data, including RCM analysis, receptor & peptide sequences, raw data…etc etc on Zenodo
(zenodo.org/records/1546... )
As usual, we have uploaded the data, including RCM analysis, receptor & peptide sequences, raw data…etc etc on Zenodo
(zenodo.org/records/1546... )
Together with structural prediction, phylogenomics data, and lots of in-planta validations, we engineered synthetic SCORE that perceive CSPs from a range of plant pathogens and pests.
September 4, 2025 at 7:33 PM
Together with structural prediction, phylogenomics data, and lots of in-planta validations, we engineered synthetic SCORE that perceive CSPs from a range of plant pathogens and pests.
With the polymorphic CSP recognition specificity, we wonder if SCOREs can be engineered.
We predicted receptor-peptide structures to narrow down the region of interest.
We predicted receptor-peptide structures to narrow down the region of interest.
September 4, 2025 at 7:33 PM
With the polymorphic CSP recognition specificity, we wonder if SCOREs can be engineered.
We predicted receptor-peptide structures to narrow down the region of interest.
We predicted receptor-peptide structures to narrow down the region of interest.
They do!
Across the 22 SOCRE orthologs we tested, most exhibit unique recognition specificity towards different CSP peptides.
Across the 22 SOCRE orthologs we tested, most exhibit unique recognition specificity towards different CSP peptides.
September 4, 2025 at 7:33 PM
They do!
Across the 22 SOCRE orthologs we tested, most exhibit unique recognition specificity towards different CSP peptides.
Across the 22 SOCRE orthologs we tested, most exhibit unique recognition specificity towards different CSP peptides.
Bioinformatic analyses indicate that CSPs are very diverse across organisms (not just found in bacteria).
We wonder if SCORE orthologs across the flowering plant lineage can perceive different CSP peptides.
We wonder if SCORE orthologs across the flowering plant lineage can perceive different CSP peptides.
September 4, 2025 at 7:33 PM
Bioinformatic analyses indicate that CSPs are very diverse across organisms (not just found in bacteria).
We wonder if SCORE orthologs across the flowering plant lineage can perceive different CSP peptides.
We wonder if SCORE orthologs across the flowering plant lineage can perceive different CSP peptides.
Unlike the previously identified PRR CORE, "181" exhibits a more selective recognition towards csp15 variants, so we named it “Selective CORE” (SCORE).
In addition, CORE and SCORE are not closely related; these receptors convergently evolved to perceive CSPs.
In addition, CORE and SCORE are not closely related; these receptors convergently evolved to perceive CSPs.
September 4, 2025 at 7:33 PM
Unlike the previously identified PRR CORE, "181" exhibits a more selective recognition towards csp15 variants, so we named it “Selective CORE” (SCORE).
In addition, CORE and SCORE are not closely related; these receptors convergently evolved to perceive CSPs.
In addition, CORE and SCORE are not closely related; these receptors convergently evolved to perceive CSPs.
We screened for chimeric receptors activated by the plant pathogen Agrobacterium, and identified the receptor “181” from Pomelo.
Following biochemical analyses, we discovered that 181 perceives cold shock protein (CSP) peptides.
Following biochemical analyses, we discovered that 181 perceives cold shock protein (CSP) peptides.
September 4, 2025 at 7:33 PM
We screened for chimeric receptors activated by the plant pathogen Agrobacterium, and identified the receptor “181” from Pomelo.
Following biochemical analyses, we discovered that 181 perceives cold shock protein (CSP) peptides.
Following biochemical analyses, we discovered that 181 perceives cold shock protein (CSP) peptides.
To characterize immune receptors (PRRs) in plants, we cluster PRRs into subgroups based on conservation of their inner LRR residues.
We obtained >1000s subgroups with this approach, and cloned ~210s into receptor chimeras for further characterization.
We obtained >1000s subgroups with this approach, and cloned ~210s into receptor chimeras for further characterization.
September 4, 2025 at 7:33 PM
To characterize immune receptors (PRRs) in plants, we cluster PRRs into subgroups based on conservation of their inner LRR residues.
We obtained >1000s subgroups with this approach, and cloned ~210s into receptor chimeras for further characterization.
We obtained >1000s subgroups with this approach, and cloned ~210s into receptor chimeras for further characterization.
First post on Bluesky!! ☀️
Very happy to share that my main postdoc work ‘Systematic Discovery and Design of Synthetic Immune Receptors in Plants’ is finally out on Biorxiv!
www.biorxiv.org/content/10.1...
The full story is summarized here:
Very happy to share that my main postdoc work ‘Systematic Discovery and Design of Synthetic Immune Receptors in Plants’ is finally out on Biorxiv!
www.biorxiv.org/content/10.1...
The full story is summarized here:
Systematic Discovery and Design of Synthetic Immune Receptors in Plants
Plants deploy a diverse array of pattern recognition receptors (PRRs) that perceive microbe-associated molecular patterns (MAMPs) to activate immune responses. Leucine-rich repeat receptor-like kinase...
www.biorxiv.org
September 4, 2025 at 7:33 PM
Yes! We are close to getting Arabidopsis transgenic lines that would recognize CSP from clubroot. Would be great to colab and test it!
March 18, 2025 at 3:16 AM
Yes! We are close to getting Arabidopsis transgenic lines that would recognize CSP from clubroot. Would be great to colab and test it!
This has truly been one of my fav projects. Huge thanks to Michele & Marc, Takehiro & Dohmae-sensei for the bioinformatic & MS support, @yasukadota.bsky.social & @shirasulab.bsky.social for their unwavering support & belief in me & this project. Hope you enjoyed the thread & happy to discuss!
March 18, 2025 at 2:01 AM
This has truly been one of my fav projects. Huge thanks to Michele & Marc, Takehiro & Dohmae-sensei for the bioinformatic & MS support, @yasukadota.bsky.social & @shirasulab.bsky.social for their unwavering support & belief in me & this project. Hope you enjoyed the thread & happy to discuss!
To summarize, we:
-Designed a system to characterize the pathogen recognition landscape of LRR-RLK-XIIs in plants
-Identified an ancient but lineage-specific PRR that exhibits remarkable polymorphism in MAMP recognition
-Designed PRRs to perceive specific MAMPs from multiple crop pathogens
-Designed a system to characterize the pathogen recognition landscape of LRR-RLK-XIIs in plants
-Identified an ancient but lineage-specific PRR that exhibits remarkable polymorphism in MAMP recognition
-Designed PRRs to perceive specific MAMPs from multiple crop pathogens
March 18, 2025 at 2:01 AM
To summarize, we:
-Designed a system to characterize the pathogen recognition landscape of LRR-RLK-XIIs in plants
-Identified an ancient but lineage-specific PRR that exhibits remarkable polymorphism in MAMP recognition
-Designed PRRs to perceive specific MAMPs from multiple crop pathogens
-Designed a system to characterize the pathogen recognition landscape of LRR-RLK-XIIs in plants
-Identified an ancient but lineage-specific PRR that exhibits remarkable polymorphism in MAMP recognition
-Designed PRRs to perceive specific MAMPs from multiple crop pathogens
Through this mini scan, we designed SCORE variants that robustly perceives CSPs from many devastating crop pathogens. Hopefully these would improve disease resistance against pathogens in crops
(XXI)
(XXI)
March 18, 2025 at 2:01 AM
Through this mini scan, we designed SCORE variants that robustly perceives CSPs from many devastating crop pathogens. Hopefully these would improve disease resistance against pathogens in crops
(XXI)
(XXI)
We did a mini-mutational scan on these 3 AAs, generated 37 SCORE variants and tested them against 104 csp15 peptides. To a certain extent, the surface charges in this region determine csp15 recognition specificity in SCORE
(XX)
(XX)
March 18, 2025 at 2:01 AM
We did a mini-mutational scan on these 3 AAs, generated 37 SCORE variants and tested them against 104 csp15 peptides. To a certain extent, the surface charges in this region determine csp15 recognition specificity in SCORE
(XX)
(XX)
Due to the AF prediction of ionic bonds between csp15 & 10th LRR of SCORE, we noticed that the surface charges in this region are different between SCORE orthologs. We can also drastically modify the surface charges, simply by mutating 3 AA residues in the 10th LRR
(XIX)
(XIX)
March 18, 2025 at 2:01 AM
Due to the AF prediction of ionic bonds between csp15 & 10th LRR of SCORE, we noticed that the surface charges in this region are different between SCORE orthologs. We can also drastically modify the surface charges, simply by mutating 3 AA residues in the 10th LRR
(XIX)
(XIX)
This indicates that 8th-11th LRR determines csp15 recognition specificity in SCOREs. We perform a bunch of LRR swaps between orthologs and narrow it further down to the 10th LRR. csp15 recognition specificity can, in an extend, be transferred between SCORE orthologs
(XVIII)
(XVIII)
March 18, 2025 at 2:01 AM
This indicates that 8th-11th LRR determines csp15 recognition specificity in SCOREs. We perform a bunch of LRR swaps between orthologs and narrow it further down to the 10th LRR. csp15 recognition specificity can, in an extend, be transferred between SCORE orthologs
(XVIII)
(XVIII)
These predictions became convincing when combined with phylogenomics. While the AAs that interacts with csp15 in 3rd-7th & 12th-16thLRR are relatively conserved between SCORE orthologs, the LRR 8th-11th AAs that interacts with the variable region of csp15 are also variable
(XVII)
(XVII)
March 18, 2025 at 2:01 AM
These predictions became convincing when combined with phylogenomics. While the AAs that interacts with csp15 in 3rd-7th & 12th-16thLRR are relatively conserved between SCORE orthologs, the LRR 8th-11th AAs that interacts with the variable region of csp15 are also variable
(XVII)
(XVII)