8/10

In conclusion, our work provides an innovative framework for dissecting the crosstalk between gene regulation and inter-tissue communication in mammalian embryonic development.

7/10

Finally, we used chimeric gastruloids derived from a mix of wild-type (WT) and MSGN1 KO cells. This revealed that MSGN1 KO cells can form spinal cord in the presence of WT cells.
This indicates an underlying inter-tissue communication program driven by MSGN1.

6/10

Surprisingly, MSGN1 KO not only impaired mesoderm development, as anticipated, but also negatively affected spinal cord development. This reveals an inter-tissue role of MSGN1 that was previously unknown.

4/10

Next, we investigated which transcription factors (TFs) are active during gastruloid development. To do this, we defined the developmental trajectories in gastruloids and predicted TF activity based on their motif accessibility.

3/10

We discovered that the chromatin regulatory profile of gastruloids strikingly matches that of post-gastrulation embryos.

2/10

We utilized single-cell ATAC-seq to analyze developing mouse gastruloids, successfully defining their chromatin regulatory profile.