Jesse Boehm
@boehmjesse.bsky.social
Chief Science Officer, Break Through Cancer; Principal Investigator, Koch Institute, MIT - boehmlab.org
Reposted by Jesse Boehm
So, we teamed up with Anne and Paul’s lab, along with Calvin Jan at Calico, with the aim of building an accessible & unbiased high-content cell profiling platform that could be applied to genome-scale CRISPR screens (Project PERISCOPE).
January 27, 2025 at 6:23 PM
So, we teamed up with Anne and Paul’s lab, along with Calvin Jan at Calico, with the aim of building an accessible & unbiased high-content cell profiling platform that could be applied to genome-scale CRISPR screens (Project PERISCOPE).
Reposted by Jesse Boehm
We’re big fans of using expression-based profiling (specifically Perturb-seq) to generate high-dimensional phenotypic profiles of perturbed cells, and w/ @oana-ursu.bsky.social & @boehmjesse.bsky.social have used it successfully for generating variant effect maps (www.nature.com/articles/s41...)
Massively parallel phenotyping of coding variants in cancer with Perturb-seq - Nature Biotechnology
The functional impact of somatic mutations in cancer genes is determined by pooled Perturb-seq.
www.nature.com
January 27, 2025 at 6:23 PM
We’re big fans of using expression-based profiling (specifically Perturb-seq) to generate high-dimensional phenotypic profiles of perturbed cells, and w/ @oana-ursu.bsky.social & @boehmjesse.bsky.social have used it successfully for generating variant effect maps (www.nature.com/articles/s41...)
This is such an important new technology for the future of CRISPR functional genomics for biomedicine. Congrats to @jtneal.bsky.social @drannecarpenter.bsky.social Paul Blainey and their amazing labs for this tour-de-force success!
January 27, 2025 at 5:43 PM
This is such an important new technology for the future of CRISPR functional genomics for biomedicine. Congrats to @jtneal.bsky.social @drannecarpenter.bsky.social Paul Blainey and their amazing labs for this tour-de-force success!