Andrew Savinov
@biosavinov.bsky.social
Biophysicist using single-molecule & high-throughput methods to interrogate sequence-structure-function. K99 fellow at MIT w/ Gene-Wei Li and Stan Fields; PhD w/ Steve Block at Stanford.
We tested ~1800 designs experimentally in living cells alongside fragments tiling across DNA gyrase. About 20% inhibited bacterial growth, and 5.5% were specific to the designed binding mode, which we assayed by almost 1800 mutants breaking the peptide binding interfaces! (3/n)
October 27, 2025 at 12:39 PM
We tested ~1800 designs experimentally in living cells alongside fragments tiling across DNA gyrase. About 20% inhibited bacterial growth, and 5.5% were specific to the designed binding mode, which we assayed by almost 1800 mutants breaking the peptide binding interfaces! (3/n)