Bio-protocol
@bio-protocol.bsky.social
Bio-protocol (www.bio-protocol.org) was founded in 2011 by a group of researchers at Stanford University with a singular mission: Improving research reproducibility by publishing high-quality protocols in the life sciences.
Bio-protocol's new issue is now live! 🎉
bio-protocol.org/en/archive?v...
bio-protocol.org/en/archive?v...
November 21, 2025 at 2:15 AM
Bio-protocol's new issue is now live! 🎉
bio-protocol.org/en/archive?v...
bio-protocol.org/en/archive?v...
Image-Based Profiling in Live Cells Using Live Cell Painting
Work by Thaís Moraes-Lacerda etal. at Universidade Estadual de Campinas (UNICAMP) and Shantanu Singh at Broad Institute of MIT and Harvard.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
Work by Thaís Moraes-Lacerda etal. at Universidade Estadual de Campinas (UNICAMP) and Shantanu Singh at Broad Institute of MIT and Harvard.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
November 20, 2025 at 5:20 AM
Image-Based Profiling in Live Cells Using Live Cell Painting
Work by Thaís Moraes-Lacerda etal. at Universidade Estadual de Campinas (UNICAMP) and Shantanu Singh at Broad Institute of MIT and Harvard.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
Work by Thaís Moraes-Lacerda etal. at Universidade Estadual de Campinas (UNICAMP) and Shantanu Singh at Broad Institute of MIT and Harvard.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
Fluorescence Lifetime-Based Separation of FAST-Labeled Cellular Compartment:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
November 19, 2025 at 8:51 AM
Fluorescence Lifetime-Based Separation of FAST-Labeled Cellular Compartment:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
A Reliable In Planta Inoculation and Antifungal Screening Protocol for Rhizoctonia solani-Induced Sheath Blight in Rice:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
November 19, 2025 at 2:39 AM
A Reliable In Planta Inoculation and Antifungal Screening Protocol for Rhizoctonia solani-Induced Sheath Blight in Rice:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
Generation of 3D Human iPSC-Derived Multi-Cell Type Neurospheres for Studying Neuron, Astrocyte, and Microglia Crosstalk:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
November 18, 2025 at 8:52 AM
Generation of 3D Human iPSC-Derived Multi-Cell Type Neurospheres for Studying Neuron, Astrocyte, and Microglia Crosstalk:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
A Practical CRISPR-Based Method for Rapid Genome Editing in Caulobacter crescentus
Work by Xuezhou Yuan, Xin Yu, Wei Zhao and Jingxian Sun at Shenzhen Institutes of Advanced Technology.
#LifeSciences #Reproducibility #Microbiology
Work by Xuezhou Yuan, Xin Yu, Wei Zhao and Jingxian Sun at Shenzhen Institutes of Advanced Technology.
#LifeSciences #Reproducibility #Microbiology
November 18, 2025 at 1:58 AM
A Practical CRISPR-Based Method for Rapid Genome Editing in Caulobacter crescentus
Work by Xuezhou Yuan, Xin Yu, Wei Zhao and Jingxian Sun at Shenzhen Institutes of Advanced Technology.
#LifeSciences #Reproducibility #Microbiology
Work by Xuezhou Yuan, Xin Yu, Wei Zhao and Jingxian Sun at Shenzhen Institutes of Advanced Technology.
#LifeSciences #Reproducibility #Microbiology
Optimized Protocol for the Collection, Cryopreservation, and In Vitro Cultivation of Human Gut Microbiota for Toxicomicrobiomics Applications:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
November 17, 2025 at 9:05 AM
Optimized Protocol for the Collection, Cryopreservation, and In Vitro Cultivation of Human Gut Microbiota for Toxicomicrobiomics Applications:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
📢 Bio-protocol Webinars present: Functional Diversity of Type I Interferons in Immune Responses
🔗 Register: s.bio-protocol.org/8ad9797eee9a...
Join us on:
🗓 Date: December 4th, 2025
⏰ Time: 1:00 PM EST
Speaker: Dr. Kathrin Sutter
Moderator: Dr. Marina Kort
This #webinar is supported by BioLegend.
🔗 Register: s.bio-protocol.org/8ad9797eee9a...
Join us on:
🗓 Date: December 4th, 2025
⏰ Time: 1:00 PM EST
Speaker: Dr. Kathrin Sutter
Moderator: Dr. Marina Kort
This #webinar is supported by BioLegend.
November 17, 2025 at 9:03 AM
📢 Bio-protocol Webinars present: Functional Diversity of Type I Interferons in Immune Responses
🔗 Register: s.bio-protocol.org/8ad9797eee9a...
Join us on:
🗓 Date: December 4th, 2025
⏰ Time: 1:00 PM EST
Speaker: Dr. Kathrin Sutter
Moderator: Dr. Marina Kort
This #webinar is supported by BioLegend.
🔗 Register: s.bio-protocol.org/8ad9797eee9a...
Join us on:
🗓 Date: December 4th, 2025
⏰ Time: 1:00 PM EST
Speaker: Dr. Kathrin Sutter
Moderator: Dr. Marina Kort
This #webinar is supported by BioLegend.
Protocol for the Isolation and Analysis of Extracellular Vesicles From Peripheral Blood: Red Cell, Endothelial, and Platelet-Derived Extracellular Vesicles:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
November 17, 2025 at 2:28 AM
Protocol for the Isolation and Analysis of Extracellular Vesicles From Peripheral Blood: Red Cell, Endothelial, and Platelet-Derived Extracellular Vesicles:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
Production of Genetically Engineered Extracellular Vesicles for Targeted Protein Delivery:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
November 14, 2025 at 8:08 AM
Production of Genetically Engineered Extracellular Vesicles for Targeted Protein Delivery:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
Labeling Postsynaptic Densities for Super-Resolution Microscopy With Minimal Signal-Loss and Offset:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
November 14, 2025 at 2:34 AM
Labeling Postsynaptic Densities for Super-Resolution Microscopy With Minimal Signal-Loss and Offset:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
Bio-protocol is proud to feature one of the latest contributions from Professor Sakaguchi's team and colleagues: single-cell mass cytometry method for precise Treg analysis.
🔗https://s.bio-protocol.org/b7c5b7042af063ec
(2/3)
🔗https://s.bio-protocol.org/b7c5b7042af063ec
(2/3)
November 13, 2025 at 8:55 AM
Bio-protocol is proud to feature one of the latest contributions from Professor Sakaguchi's team and colleagues: single-cell mass cytometry method for precise Treg analysis.
🔗https://s.bio-protocol.org/b7c5b7042af063ec
(2/3)
🔗https://s.bio-protocol.org/b7c5b7042af063ec
(2/3)
Optogenetic Approach for Investigating Descending Control of Nociception in Ex Vivo Spinal Cord Preparation
Work by Volodymyr Krotov etal. at Bogomoletz Institute of Physiology and Nana Voitenko at Kyiv Academic University.
#LifeSciences #Reproducibility #Neuroscience
Work by Volodymyr Krotov etal. at Bogomoletz Institute of Physiology and Nana Voitenko at Kyiv Academic University.
#LifeSciences #Reproducibility #Neuroscience
November 13, 2025 at 7:13 AM
Optogenetic Approach for Investigating Descending Control of Nociception in Ex Vivo Spinal Cord Preparation
Work by Volodymyr Krotov etal. at Bogomoletz Institute of Physiology and Nana Voitenko at Kyiv Academic University.
#LifeSciences #Reproducibility #Neuroscience
Work by Volodymyr Krotov etal. at Bogomoletz Institute of Physiology and Nana Voitenko at Kyiv Academic University.
#LifeSciences #Reproducibility #Neuroscience
Computational Workflow for Genome-Wide DNA Methylation Profiling and Differential Methylation Analysis
Work by Pei-Yu Lin etal. at Institute of Plant and Microbial Biology.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
Work by Pei-Yu Lin etal. at Institute of Plant and Microbial Biology.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
November 13, 2025 at 1:47 AM
Computational Workflow for Genome-Wide DNA Methylation Profiling and Differential Methylation Analysis
Work by Pei-Yu Lin etal. at Institute of Plant and Microbial Biology.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
Work by Pei-Yu Lin etal. at Institute of Plant and Microbial Biology.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
A Quantitative Spectrophotometric Assay Matched With Environmental Scanning Electron Microscopy to Measure Calcium Crystals in Human Osteoarthritic Synovial Fluid:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
November 12, 2025 at 9:34 AM
A Quantitative Spectrophotometric Assay Matched With Environmental Scanning Electron Microscopy to Measure Calcium Crystals in Human Osteoarthritic Synovial Fluid:
bio-protocol.org/en/bpdetail?...
bio-protocol.org/en/bpdetail?...
Library Preparation for Genome-Wide DNA Methylation Profiling
Work by Fei-Man Hsu and Matteo Pellegrini at University of California Los Angeles and Pao-Yang Chen at Institute of Plant and Microbial Biolog.
#LifeSciences #Reproducibility #MolecularBiology
Work by Fei-Man Hsu and Matteo Pellegrini at University of California Los Angeles and Pao-Yang Chen at Institute of Plant and Microbial Biolog.
#LifeSciences #Reproducibility #MolecularBiology
November 12, 2025 at 3:48 AM
Library Preparation for Genome-Wide DNA Methylation Profiling
Work by Fei-Man Hsu and Matteo Pellegrini at University of California Los Angeles and Pao-Yang Chen at Institute of Plant and Microbial Biolog.
#LifeSciences #Reproducibility #MolecularBiology
Work by Fei-Man Hsu and Matteo Pellegrini at University of California Los Angeles and Pao-Yang Chen at Institute of Plant and Microbial Biolog.
#LifeSciences #Reproducibility #MolecularBiology
Colocalizing Telomeres With PML or γH2AX Foci by IF-FISH in Mouse Brain Neurons
Work by Anna Konopka at Flinders University.
#LifeSciences #Reproducibility #Neuroscience
Work by Anna Konopka at Flinders University.
#LifeSciences #Reproducibility #Neuroscience
November 11, 2025 at 10:27 AM
Colocalizing Telomeres With PML or γH2AX Foci by IF-FISH in Mouse Brain Neurons
Work by Anna Konopka at Flinders University.
#LifeSciences #Reproducibility #Neuroscience
Work by Anna Konopka at Flinders University.
#LifeSciences #Reproducibility #Neuroscience
Live-Cell Monitoring of Piecemeal Chloroplast Autophagy
Work by Masanori Izumi, Sakuya Nakamura and Shinya Hagihara at RIKEN Center for Sustainable Resource Science (CSRS).
#LifeSciences #Reproducibility #PlantScience
Work by Masanori Izumi, Sakuya Nakamura and Shinya Hagihara at RIKEN Center for Sustainable Resource Science (CSRS).
#LifeSciences #Reproducibility #PlantScience
November 11, 2025 at 2:03 AM
Live-Cell Monitoring of Piecemeal Chloroplast Autophagy
Work by Masanori Izumi, Sakuya Nakamura and Shinya Hagihara at RIKEN Center for Sustainable Resource Science (CSRS).
#LifeSciences #Reproducibility #PlantScience
Work by Masanori Izumi, Sakuya Nakamura and Shinya Hagihara at RIKEN Center for Sustainable Resource Science (CSRS).
#LifeSciences #Reproducibility #PlantScience
Examining the Roles of m(6)A Sites in mRNA Using the Luciferase Gene Fused With Mutated RRACH Motifs
Work by Nobuko Katoku-Kikyo and Nobuaki Kikyo at University of Minnesota.
#LifeSciences #Reproducibility #MolecularBiology
Work by Nobuko Katoku-Kikyo and Nobuaki Kikyo at University of Minnesota.
#LifeSciences #Reproducibility #MolecularBiology
November 10, 2025 at 11:24 AM
Examining the Roles of m(6)A Sites in mRNA Using the Luciferase Gene Fused With Mutated RRACH Motifs
Work by Nobuko Katoku-Kikyo and Nobuaki Kikyo at University of Minnesota.
#LifeSciences #Reproducibility #MolecularBiology
Work by Nobuko Katoku-Kikyo and Nobuaki Kikyo at University of Minnesota.
#LifeSciences #Reproducibility #MolecularBiology
In Silico Prediction and In Vitro Validation of Bacterial Interactions in the Plant Rhizosphere Using a Synthetic Bacterial Community
Work by Arijit Mukherjee, Boon H. Tan and Sanjay Swarup at National University of Singapore.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
Work by Arijit Mukherjee, Boon H. Tan and Sanjay Swarup at National University of Singapore.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
November 10, 2025 at 5:54 AM
In Silico Prediction and In Vitro Validation of Bacterial Interactions in the Plant Rhizosphere Using a Synthetic Bacterial Community
Work by Arijit Mukherjee, Boon H. Tan and Sanjay Swarup at National University of Singapore.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
Work by Arijit Mukherjee, Boon H. Tan and Sanjay Swarup at National University of Singapore.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
Cluster FLISA—A Method to Compare Protein Expression Efficiency Between Cell Lines and Subunit Clustering of Proteins
Work by Sabrina Brockmöller and Lara Maria Molitor at Bundeswehr Institute of Pharmacology and Toxicology.
#LifeSciences #Reproducibility #Biochemistry
Work by Sabrina Brockmöller and Lara Maria Molitor at Bundeswehr Institute of Pharmacology and Toxicology.
#LifeSciences #Reproducibility #Biochemistry
November 7, 2025 at 9:27 AM
Cluster FLISA—A Method to Compare Protein Expression Efficiency Between Cell Lines and Subunit Clustering of Proteins
Work by Sabrina Brockmöller and Lara Maria Molitor at Bundeswehr Institute of Pharmacology and Toxicology.
#LifeSciences #Reproducibility #Biochemistry
Work by Sabrina Brockmöller and Lara Maria Molitor at Bundeswehr Institute of Pharmacology and Toxicology.
#LifeSciences #Reproducibility #Biochemistry
Lipid-Mediated Sequential Recruitment of Proteins Via Dual SLIPT and Dual SLIPT(NVOC) in Live Cells
Work by Kristina V. Bayer and Richard Wombacher at Max Planck Institute for Medical Research.
#LifeSciences #Reproducibility #Biochemistry
Work by Kristina V. Bayer and Richard Wombacher at Max Planck Institute for Medical Research.
#LifeSciences #Reproducibility #Biochemistry
November 7, 2025 at 2:11 AM
Lipid-Mediated Sequential Recruitment of Proteins Via Dual SLIPT and Dual SLIPT(NVOC) in Live Cells
Work by Kristina V. Bayer and Richard Wombacher at Max Planck Institute for Medical Research.
#LifeSciences #Reproducibility #Biochemistry
Work by Kristina V. Bayer and Richard Wombacher at Max Planck Institute for Medical Research.
#LifeSciences #Reproducibility #Biochemistry
Bio-protocol's new issue is now live! 🎉
bio-protocol.org/en/archive?v...
bio-protocol.org/en/archive?v...
November 6, 2025 at 6:05 AM
Bio-protocol's new issue is now live! 🎉
bio-protocol.org/en/archive?v...
bio-protocol.org/en/archive?v...
Direct Plant Regeneration From Immature Male Inflorescence of Banana (Musa spp.)
Work by Pradeep Chand Deo from Australia.
#LifeSciences #Reproducibility #PlantScience
Work by Pradeep Chand Deo from Australia.
#LifeSciences #Reproducibility #PlantScience
November 5, 2025 at 7:58 AM
Direct Plant Regeneration From Immature Male Inflorescence of Banana (Musa spp.)
Work by Pradeep Chand Deo from Australia.
#LifeSciences #Reproducibility #PlantScience
Work by Pradeep Chand Deo from Australia.
#LifeSciences #Reproducibility #PlantScience
Generation of Insulin-Producing Alpha TC1-6 Cells Using EpiCRISPR System for Targeted DNA Methylation
Work by Marija B. Đorđević , Stefan Ž. Marković Hadžić, Jovana J. Rajić and Ana D. Sarić etal. at University of Belgrade.
#LifeSciences #Reproducibility #CellBiology
Work by Marija B. Đorđević , Stefan Ž. Marković Hadžić, Jovana J. Rajić and Ana D. Sarić etal. at University of Belgrade.
#LifeSciences #Reproducibility #CellBiology
November 4, 2025 at 10:11 AM
Generation of Insulin-Producing Alpha TC1-6 Cells Using EpiCRISPR System for Targeted DNA Methylation
Work by Marija B. Đorđević , Stefan Ž. Marković Hadžić, Jovana J. Rajić and Ana D. Sarić etal. at University of Belgrade.
#LifeSciences #Reproducibility #CellBiology
Work by Marija B. Đorđević , Stefan Ž. Marković Hadžić, Jovana J. Rajić and Ana D. Sarić etal. at University of Belgrade.
#LifeSciences #Reproducibility #CellBiology