Bert van de Kooij
@bertkooij.bsky.social
Cancer researcher @UMCG, Groningen, the Netherlands | DNA double strand break repair
Interesting read. Aligns well with our findings (doi.org/10.1038/s414..., together with @haicovanattikum.bsky.social). We found DNA-PKcs inhibition to increase Cas9 break repair by single strand annealing (SSA), causing kilobase sized deletions. Exo1 depletion prevented this increase in SSA.
Multi-pathway DNA-repair reporters reveal competition between end-joining, single-strand annealing and homologous recombination at Cas9-induced DNA double-strand breaks - Nature Communications
Correct repair of broken DNA molecules is required to prevent potentially oncogenic mutations. To study repair fidelity and mechanism, van de Kooij et al. developed single cell reporters that detect i...
doi.org
December 4, 2024 at 10:17 AM
Interesting read. Aligns well with our findings (doi.org/10.1038/s414..., together with @haicovanattikum.bsky.social). We found DNA-PKcs inhibition to increase Cas9 break repair by single strand annealing (SSA), causing kilobase sized deletions. Exo1 depletion prevented this increase in SSA.