#malecns (collaborative effort with @janelia-flyem.bsky.social @camzoology.bsky.social @mrclmb.bsky.social @jefferis.bsky.social @stuartberg.bsky.social @beckett14.bsky.social @mmcosta.bsky.social Philipp Schlegel and others) split P1/pC1x into 48 cell types based on morphology and connectivity. 3/

Statistical analysis of connectivity within the central brain shows that around 6.5% of synaptic connections in the male and 1.3% of connections in the female are dimorphic. In the male, sex-specific circuit elements form highly interconnected clusters with similar connectivity. 9/10

Comparing the EM neurons to light microscopy datasets of fruitless and doublesex expression, we annotated the putative expression pattern of these genes for both the male and female connectomes. 7/10

Using these annotations we ran end-to-end analyses going from sensory inputs all the way to motor outputs. This showed, for instance, that ascending and descending neurons are not passive relays but likely perform complex transformations of information flowing between brain and nerve cord. 4/10