We integrated our atlas with the connectome using shared features, such as lineage, segment, molecular info (i.e. neurotransmitter, Notch) and neurogenesis timing. We mapped 122 transcriptional pools, each one containing an average of 26 anatomically defined neuron types. 4/8

With this atlas in place, we mapped sex differences in the atlas and adult connectomes, identifying female-specific apoptosis and transcriptional divergence as drivers of sexually dimorphic development. 7/8

Thanks to all authors and support from #MRC_LMB, @neurohub-cnr.bsky.social , @camzoology.bsky.social , #flyconnectome, @wellcometrust.bsky.social , ‪@erc.europa.eu‬, @astra-zeneca.bsky.social and @cruk-ci.bsky.social. FIN

Ah! Wei has already done a great job of this, credits at the end of this thread: bsky.app/profile/darb...

The dataset includes ~160,000 cells, segmented nuclei and mitochondria, synapses and neurotransmitter predictions, and annotations linking the CNS to peripheral sensory, motor, and visceral systems.

Info: banc.community
Data: codex.flywire.ai?dataset=banc
Viewer: ng.banc.community/view

It’s a big project and I’m taking a short holiday now after the preprint! So I’ll make another post later showing all the bits of it, and how we did this as a community !

Flywire collaborators @princetonneuro.bsky.social‬ were essential in building the connectome, and theory collaborators in interpreting it! @jdrugowitsch.bsky.social‬ Art by ‪@amysterling.bsky.social!

A massive community effort, centred on @harvardmed.bsky.social‬
@princetonneuro.bsky.social‬. EM data from @darbly.bsky.social‬ lab, collected by @jasper-tms.bsky.social‬
‪@mindyisminsu.bsky.social‬.

If you want a narrative version of this project, check out our blog post on the newly re-minted Funke Lab website!
funkelab.github.io/blog/2024/qu...

Thanks to @bobqubit.bsky.social, @asbates.bsky.social, and @jefferis.bsky.social, & @janfunkey.bsky.social for this 🦆ing awesome collaboration!

6/6