Anzy Miller
@anzymiller.bsky.social
Interested in how protein dynamics affect cell fate decisions. #DevBio #StemCells #Oscillations #Epigenetics @uniofmanchester.bsky.social
Are you enthusiastic about stem cell biology and developmental biology and looking to start a PhD in 2026? Funded opportunites now available. Contact for more infomation. Links below! @lovegrovelab.bsky.social @veronicabiga.bsky.social @manchester.ac.uk @ukri.org
October 9, 2025 at 4:21 PM
Are you enthusiastic about stem cell biology and developmental biology and looking to start a PhD in 2026? Funded opportunites now available. Contact for more infomation. Links below! @lovegrovelab.bsky.social @veronicabiga.bsky.social @manchester.ac.uk @ukri.org
4. The fold-change of the oscillations are read through an incoherent feed-forward (IFFL) gene regulatory motif. NGN3 oscillations allow for stochasticity in fold-change over time, resulting in the spreading out of differentiation over time.
July 3, 2025 at 1:35 PM
4. The fold-change of the oscillations are read through an incoherent feed-forward (IFFL) gene regulatory motif. NGN3 oscillations allow for stochasticity in fold-change over time, resulting in the spreading out of differentiation over time.
3. NGN3 oscillatory dynamics are decoded via the fold-change not expression level
The timing of cell differentiation correlates with the time of the maximum fold-change of the protein expression oscillations, not the absolute level of expression of the protein.
The timing of cell differentiation correlates with the time of the maximum fold-change of the protein expression oscillations, not the absolute level of expression of the protein.
July 3, 2025 at 1:18 PM
3. NGN3 oscillatory dynamics are decoded via the fold-change not expression level
The timing of cell differentiation correlates with the time of the maximum fold-change of the protein expression oscillations, not the absolute level of expression of the protein.
The timing of cell differentiation correlates with the time of the maximum fold-change of the protein expression oscillations, not the absolute level of expression of the protein.
2. Non-oscillatory NGN3 results in earlier differentiation
By creating a more stable version of NGN3 protein (a phosphomutant (PM)) we were able to manipulate the oscillations. Loss of repeated pulses in expression resulted in earlier activation of direct target genes and earlier differentiation.
By creating a more stable version of NGN3 protein (a phosphomutant (PM)) we were able to manipulate the oscillations. Loss of repeated pulses in expression resulted in earlier activation of direct target genes and earlier differentiation.
July 3, 2025 at 1:17 PM
2. Non-oscillatory NGN3 results in earlier differentiation
By creating a more stable version of NGN3 protein (a phosphomutant (PM)) we were able to manipulate the oscillations. Loss of repeated pulses in expression resulted in earlier activation of direct target genes and earlier differentiation.
By creating a more stable version of NGN3 protein (a phosphomutant (PM)) we were able to manipulate the oscillations. Loss of repeated pulses in expression resulted in earlier activation of direct target genes and earlier differentiation.
1. NGN3 protein expression is oscillatory in human pancreatic endocrine progenitors
NGN3, an important transcription factor for pancreatic endocrine differentiation, oscillates in its expression with a 13hr periodicity in human iPSC-derived pancreatic endocrine progenitors, irrespective of fate.
NGN3, an important transcription factor for pancreatic endocrine differentiation, oscillates in its expression with a 13hr periodicity in human iPSC-derived pancreatic endocrine progenitors, irrespective of fate.
July 3, 2025 at 1:15 PM
1. NGN3 protein expression is oscillatory in human pancreatic endocrine progenitors
NGN3, an important transcription factor for pancreatic endocrine differentiation, oscillates in its expression with a 13hr periodicity in human iPSC-derived pancreatic endocrine progenitors, irrespective of fate.
NGN3, an important transcription factor for pancreatic endocrine differentiation, oscillates in its expression with a 13hr periodicity in human iPSC-derived pancreatic endocrine progenitors, irrespective of fate.
I’m really pleased to share that our paper is out in Developmental Cell! NGN3 oscillates in the developing pancreas and controls the timing of cell differentiation via an incoherent feed-forward motif. For more details check out the comments below and DOI: 10.1016/j.devcel.2025.06.004
July 3, 2025 at 1:10 PM
I’m really pleased to share that our paper is out in Developmental Cell! NGN3 oscillates in the developing pancreas and controls the timing of cell differentiation via an incoherent feed-forward motif. For more details check out the comments below and DOI: 10.1016/j.devcel.2025.06.004
Looking foward to the Northern(ish) Developmental Biology Meeting 2025 next week @sheffielduni.bsky.social! Thanks to the organisers @shaneherbert.bsky.social Raman Das @tsakiridis.bsky.social @kyracampbelllab.bsky.social
July 3, 2025 at 8:58 AM
Looking foward to the Northern(ish) Developmental Biology Meeting 2025 next week @sheffielduni.bsky.social! Thanks to the organisers @shaneherbert.bsky.social Raman Das @tsakiridis.bsky.social @kyracampbelllab.bsky.social
In Paris this week enjoying #EMBODevTiming! Great talks so far!
May 6, 2025 at 3:27 PM
In Paris this week enjoying #EMBODevTiming! Great talks so far!