Aline Grata
@alinegrata.bsky.social
PhD student working on epithelial cell extrusion in Romain Levayer’s Lab | Social media for Piplettes Magazine ✍🏻
📍Pasteur Institute
📍Pasteur Institute
We observed that AK7 depletion leads to defects in centriole docking, with centrioles remaining sub-apical and therefore failing to nucleate motile cilia.
May 21, 2025 at 1:13 PM
We observed that AK7 depletion leads to defects in centriole docking, with centrioles remaining sub-apical and therefore failing to nucleate motile cilia.
The mechanism? Centrioles!
Cilia are anchored to the apical surface of MCCs via 150-200 centrioles, also known as basal bodies. These structures are generated close to the nucleus and then migrate apically, where they dock at the cell surface and subsequently give rise to cilia.
Cilia are anchored to the apical surface of MCCs via 150-200 centrioles, also known as basal bodies. These structures are generated close to the nucleus and then migrate apically, where they dock at the cell surface and subsequently give rise to cilia.
May 21, 2025 at 1:13 PM
The mechanism? Centrioles!
Cilia are anchored to the apical surface of MCCs via 150-200 centrioles, also known as basal bodies. These structures are generated close to the nucleus and then migrate apically, where they dock at the cell surface and subsequently give rise to cilia.
Cilia are anchored to the apical surface of MCCs via 150-200 centrioles, also known as basal bodies. These structures are generated close to the nucleus and then migrate apically, where they dock at the cell surface and subsequently give rise to cilia.
We also performed SEM imaging and observed that ciliary structures are impaired when AK7 is lacking, with reductions in both cilia number and length.
May 21, 2025 at 1:13 PM
We also performed SEM imaging and observed that ciliary structures are impaired when AK7 is lacking, with reductions in both cilia number and length.
Loss of AK7 was related to PCD-like phenotypes, with decreased fluid flow and reduced beat frequency of MCCs. One of the challenges was to find a way to visualize live ciliary beating with a good enough spatiotemporal resolution (tipically cilia are around 15μm long with beating frequency of 15Hz).
May 21, 2025 at 1:13 PM
Loss of AK7 was related to PCD-like phenotypes, with decreased fluid flow and reduced beat frequency of MCCs. One of the challenges was to find a way to visualize live ciliary beating with a good enough spatiotemporal resolution (tipically cilia are around 15μm long with beating frequency of 15Hz).
We observed that AK7 depletion leads to defects in centriole docking, with centrioles remaining sub-apical and therefore failing to nucleate motile cilia.
May 21, 2025 at 1:00 PM
We observed that AK7 depletion leads to defects in centriole docking, with centrioles remaining sub-apical and therefore failing to nucleate motile cilia.
The mechanism? Centrioles!
Cilia are anchored to the apical surface of MCCs via 150-200 centrioles, also known as basal bodies. These structures are generated close to the nucleus and then migrate apically, where they dock at the cell surface and subsequently give rise to cilia.
Cilia are anchored to the apical surface of MCCs via 150-200 centrioles, also known as basal bodies. These structures are generated close to the nucleus and then migrate apically, where they dock at the cell surface and subsequently give rise to cilia.
May 21, 2025 at 1:00 PM
The mechanism? Centrioles!
Cilia are anchored to the apical surface of MCCs via 150-200 centrioles, also known as basal bodies. These structures are generated close to the nucleus and then migrate apically, where they dock at the cell surface and subsequently give rise to cilia.
Cilia are anchored to the apical surface of MCCs via 150-200 centrioles, also known as basal bodies. These structures are generated close to the nucleus and then migrate apically, where they dock at the cell surface and subsequently give rise to cilia.
We also performed SEM imaging and observed that ciliary structures are impaired when AK7 is lacking, with reductions in both cilia number and length.
May 21, 2025 at 1:00 PM
We also performed SEM imaging and observed that ciliary structures are impaired when AK7 is lacking, with reductions in both cilia number and length.
Loss of AK7 was related to PCD-like phenotypes, with decreased fluid flow and reduced beat frequency of MCCs. One of the challenges was to find a way to visualize live ciliary beating with a good enough spatiotemporal resolution (tipically cilia are around 15μm long with beating frequency of 15Hz).
May 21, 2025 at 1:00 PM
Loss of AK7 was related to PCD-like phenotypes, with decreased fluid flow and reduced beat frequency of MCCs. One of the challenges was to find a way to visualize live ciliary beating with a good enough spatiotemporal resolution (tipically cilia are around 15μm long with beating frequency of 15Hz).
Just wrapped up an amazing 2-week Principles and Applications of Fluorescent Microscopy #PAFM course @pasteur.fr!
Built a microscope, designed a confocal, attended great talks, and got hands-on with the latest tech🔬💡
Great mix of theory and practice. 10/10, highly recommend!🤩
#microscopy #biology
Built a microscope, designed a confocal, attended great talks, and got hands-on with the latest tech🔬💡
Great mix of theory and practice. 10/10, highly recommend!🤩
#microscopy #biology
March 25, 2025 at 9:25 AM
Just wrapped up an amazing 2-week Principles and Applications of Fluorescent Microscopy #PAFM course @pasteur.fr!
Built a microscope, designed a confocal, attended great talks, and got hands-on with the latest tech🔬💡
Great mix of theory and practice. 10/10, highly recommend!🤩
#microscopy #biology
Built a microscope, designed a confocal, attended great talks, and got hands-on with the latest tech🔬💡
Great mix of theory and practice. 10/10, highly recommend!🤩
#microscopy #biology