Alex Guyon
@alexguyon.bsky.social
PhD student in the Schornack Group @slcuplants.bsky.social. Interested in plant-microbe interfaces.
https://www.slcu.cam.ac.uk/people/alex-guyon
https://www.slcu.cam.ac.uk/people/alex-guyon
8/ TL;DR:
✅ Phosphoinositide signatures at plant-microbe interfaces are dynamic
✅ Mutualist colonisation reshapes pathogen interface identity
✅ This shift correlates with enhanced resistance, suggesting membrane identity may influence infection outcome.
✅ Phosphoinositide signatures at plant-microbe interfaces are dynamic
✅ Mutualist colonisation reshapes pathogen interface identity
✅ This shift correlates with enhanced resistance, suggesting membrane identity may influence infection outcome.
June 24, 2025 at 4:54 PM
8/ TL;DR:
✅ Phosphoinositide signatures at plant-microbe interfaces are dynamic
✅ Mutualist colonisation reshapes pathogen interface identity
✅ This shift correlates with enhanced resistance, suggesting membrane identity may influence infection outcome.
✅ Phosphoinositide signatures at plant-microbe interfaces are dynamic
✅ Mutualist colonisation reshapes pathogen interface identity
✅ This shift correlates with enhanced resistance, suggesting membrane identity may influence infection outcome.
6/ Even more: co-colonisation enhances resistance to P. palmivora. Mutualist presence seems to rewire the pathogen interface.
June 24, 2025 at 4:54 PM
6/ Even more: co-colonisation enhances resistance to P. palmivora. Mutualist presence seems to rewire the pathogen interface.
5/ But here's the twist, during co-colonisation, haustoria begin recruiting PI4P
That’s a fundamental shift in membrane identity. And yes - we observed single plant cells simultaneously harbouring both an arbuscule and a haustorium!
That’s a fundamental shift in membrane identity. And yes - we observed single plant cells simultaneously harbouring both an arbuscule and a haustorium!
June 24, 2025 at 4:54 PM
5/ But here's the twist, during co-colonisation, haustoria begin recruiting PI4P
That’s a fundamental shift in membrane identity. And yes - we observed single plant cells simultaneously harbouring both an arbuscule and a haustorium!
That’s a fundamental shift in membrane identity. And yes - we observed single plant cells simultaneously harbouring both an arbuscule and a haustorium!
4/Watch it in action! Here’s PI(4,5)P₂ (via biosensor P24Y) tracking fungal growth during AM symbiosis. Captured using @jenmcgaley.bsky.social‘s AMSlide.
June 24, 2025 at 4:54 PM
4/Watch it in action! Here’s PI(4,5)P₂ (via biosensor P24Y) tracking fungal growth during AM symbiosis. Captured using @jenmcgaley.bsky.social‘s AMSlide.
3/ In single infections:
• PI(4,5)P₂ is tip-enriched in arbuscules but uniform around haustoria. Each interaction has a distinct membrane fingerprint.
• PI4P is present at the periarbuscular membrane of the mutualist but absent from haustoria of the pathogen.
• PI(4,5)P₂ is tip-enriched in arbuscules but uniform around haustoria. Each interaction has a distinct membrane fingerprint.
• PI4P is present at the periarbuscular membrane of the mutualist but absent from haustoria of the pathogen.
June 24, 2025 at 4:54 PM
3/ In single infections:
• PI(4,5)P₂ is tip-enriched in arbuscules but uniform around haustoria. Each interaction has a distinct membrane fingerprint.
• PI4P is present at the periarbuscular membrane of the mutualist but absent from haustoria of the pathogen.
• PI(4,5)P₂ is tip-enriched in arbuscules but uniform around haustoria. Each interaction has a distinct membrane fingerprint.
• PI4P is present at the periarbuscular membrane of the mutualist but absent from haustoria of the pathogen.
2/ Phosphoinositides (PIPs) are key lipids that define membrane identity. We visualised PI(4,5)P₂ and PI4P biosensors from @yvonjaillais.bsky.social in Nicotiana benthamiana during root colonisation by a mutualist AM fungus - F. mosseae and a pathogen - P. palmivora. www.biorxiv.org/content/10.1...
June 24, 2025 at 4:54 PM
2/ Phosphoinositides (PIPs) are key lipids that define membrane identity. We visualised PI(4,5)P₂ and PI4P biosensors from @yvonjaillais.bsky.social in Nicotiana benthamiana during root colonisation by a mutualist AM fungus - F. mosseae and a pathogen - P. palmivora. www.biorxiv.org/content/10.1...
1/🚨 New preprint alert! Can mutualists and pathogens co-colonise the same living plant cell and what does that do to the plant membranes that surround these microbes?
June 24, 2025 at 4:54 PM
1/🚨 New preprint alert! Can mutualists and pathogens co-colonise the same living plant cell and what does that do to the plant membranes that surround these microbes?