Alexandre Mayran
@alexandremayran.bsky.social
Interestingly, this is reminiscent of a study in 2000, where Correia and Conlon reported (www.sciencedirect.com/science/arti...) that tail bud explants only form epithelial somite when both mesoderm and surface ectoderm are present. 8/12
November 17, 2025 at 10:37 AM
Interestingly, this is reminiscent of a study in 2000, where Correia and Conlon reported (www.sciencedirect.com/science/arti...) that tail bud explants only form epithelial somite when both mesoderm and surface ectoderm are present. 8/12
Surprisingly, we found that WT cells in the anterior portion establish an apico-basal polarity, with the basal side facing N-Cadherin mutant cells similarly to what Matrigel would normally cause. 7/12
November 17, 2025 at 10:37 AM
Surprisingly, we found that WT cells in the anterior portion establish an apico-basal polarity, with the basal side facing N-Cadherin mutant cells similarly to what Matrigel would normally cause. 7/12
This coherent organization even leads to the formation of polarized somitic tissue, despite the WT cells normally requiring Matrigel for this. 6/12
November 17, 2025 at 10:37 AM
This coherent organization even leads to the formation of polarized somitic tissue, despite the WT cells normally requiring Matrigel for this. 6/12
How are these organized? N-Cadherin expressing cells are dispersed along the entire A-P axis forming a network of connected cells. However, tissue organization is much more coherent, suggesting that cell fate organization can dominate over N-Cadherin expression. 5/12
November 17, 2025 at 10:37 AM
How are these organized? N-Cadherin expressing cells are dispersed along the entire A-P axis forming a network of connected cells. However, tissue organization is much more coherent, suggesting that cell fate organization can dominate over N-Cadherin expression. 5/12
We generated mosaic gastruloids with varying proportion of mutant cells to force differential N-Cadherin expression. Interestingly, the elongation was directly correlated to the proportion of mutant cells, linking gastruloid elongation to cell adhesion. 4/12
November 17, 2025 at 10:37 AM
We generated mosaic gastruloids with varying proportion of mutant cells to force differential N-Cadherin expression. Interestingly, the elongation was directly correlated to the proportion of mutant cells, linking gastruloid elongation to cell adhesion. 4/12
However, unlike TLS which rely on Matrigel, this occurs without any epithelialization. 3/12
November 17, 2025 at 10:37 AM
However, unlike TLS which rely on Matrigel, this occurs without any epithelialization. 3/12
Here, we could confirm that gastruloids lacking N-Cadherin self-organize correctly, in fact even better than their wildtype counter part as they form polarized somitic tissue without requiring exogeneous extra cellular matrix such as matrigel. 2/12
November 17, 2025 at 10:37 AM
Here, we could confirm that gastruloids lacking N-Cadherin self-organize correctly, in fact even better than their wildtype counter part as they form polarized somitic tissue without requiring exogeneous extra cellular matrix such as matrigel. 2/12
Finally, using resizing experiments, we show that the gastruloids morphology and patterning have a limited memory of their initial size, instead following their effective size.
December 24, 2024 at 3:47 PM
Finally, using resizing experiments, we show that the gastruloids morphology and patterning have a limited memory of their initial size, instead following their effective size.
While cell fate is weakly impacted, hypoxia is largely induced in large gastruloids.
December 24, 2024 at 3:47 PM
While cell fate is weakly impacted, hypoxia is largely induced in large gastruloids.
In fact, withing a broad range of size, despite major morphological differences, very few transcriptional changes can be observed
December 24, 2024 at 3:47 PM
In fact, withing a broad range of size, despite major morphological differences, very few transcriptional changes can be observed
Then we wondered, how would this impact transcription, would it reflect gastruloid's temporality or morphology? In contrast with morphology, the emergence of a Mesp2 pole was incredibly tight between different sizes #NotintheGenes?
December 24, 2024 at 3:47 PM
Then we wondered, how would this impact transcription, would it reflect gastruloid's temporality or morphology? In contrast with morphology, the emergence of a Mesp2 pole was incredibly tight between different sizes #NotintheGenes?
However, large gastruloids take longer to establish their elongated morphologies, while smaller one are faster, in fact this temporal association between size and morphology is linear
December 24, 2024 at 3:47 PM
However, large gastruloids take longer to establish their elongated morphologies, while smaller one are faster, in fact this temporal association between size and morphology is linear
In the original gastruloid paper (van den Brink et al, 2014), it was already clear that larger gastruloids formed multi-axial structures. However, we noticed that sometimes, even extreme sizes can make it just fine
December 24, 2024 at 3:47 PM
In the original gastruloid paper (van den Brink et al, 2014), it was already clear that larger gastruloids formed multi-axial structures. However, we noticed that sometimes, even extreme sizes can make it just fine
This started with the observation that there's multiple ways to make gastruloids, sometimes they're well behaved, sometimes they're on the naughty list
December 24, 2024 at 3:47 PM
This started with the observation that there's multiple ways to make gastruloids, sometimes they're well behaved, sometimes they're on the naughty list
Finally, using resizing experiments, we show that the gastruloids morphology and patterning have a limited memory of their initial size, instead following their effective size
December 24, 2024 at 2:35 PM
Finally, using resizing experiments, we show that the gastruloids morphology and patterning have a limited memory of their initial size, instead following their effective size
While cell fate is weakly impacted, hypoxia is largely induced in large gastruloids
December 24, 2024 at 2:35 PM
While cell fate is weakly impacted, hypoxia is largely induced in large gastruloids
In fact, withing a broad range of size, despite major morphological differences, very few transcriptional changes can be observed.
December 24, 2024 at 2:35 PM
In fact, withing a broad range of size, despite major morphological differences, very few transcriptional changes can be observed.
Then we wondered, how would this impact transcription, would it reflect gastruloid's temporality or morphology? In contrast with morphology, the emergence of a Mesp2 pole was incredibly tight between different sizes #NotintheGenes?
December 24, 2024 at 2:35 PM
Then we wondered, how would this impact transcription, would it reflect gastruloid's temporality or morphology? In contrast with morphology, the emergence of a Mesp2 pole was incredibly tight between different sizes #NotintheGenes?
However, large gastruloids take longer to establish their elongated morphologies, while smaller one are faster, in fact this temporal association between size and morphology is linear
December 24, 2024 at 2:35 PM
However, large gastruloids take longer to establish their elongated morphologies, while smaller one are faster, in fact this temporal association between size and morphology is linear
In the original gastruloid paper (van den Brink et al., 2014), it was already clear that larger gastruloids formed multi-axial structures. However, we noticed that sometimes even extreme sizes can make it just fine
December 24, 2024 at 2:35 PM
In the original gastruloid paper (van den Brink et al., 2014), it was already clear that larger gastruloids formed multi-axial structures. However, we noticed that sometimes even extreme sizes can make it just fine
This started with the observation that there's multiple ways to make gastruloids, sometimes they're well behaved, sometimes they're on the naughty list
December 24, 2024 at 2:35 PM
This started with the observation that there's multiple ways to make gastruloids, sometimes they're well behaved, sometimes they're on the naughty list
Finally, using resizing experiments, we show that the gastruloids morphology and patterning have a limited memory of their initial size, instead following their effective size.
December 24, 2024 at 1:36 PM
Finally, using resizing experiments, we show that the gastruloids morphology and patterning have a limited memory of their initial size, instead following their effective size.
While cell fate is weakly impacted, hypoxia is largely induced in large gastruloids
December 24, 2024 at 1:36 PM
While cell fate is weakly impacted, hypoxia is largely induced in large gastruloids
In fact, withing a broad range of size, despite major morphological differences, very few transcriptional changes can be observed
December 24, 2024 at 1:36 PM
In fact, withing a broad range of size, despite major morphological differences, very few transcriptional changes can be observed
Then we wondered, how would this impact transcription, would it reflect gastruloid's temporality or morphology? In contrast with morphology, the emergence of a Mesp2 pole was incredibly tight between different sizes #NotintheGenes?
December 24, 2024 at 1:36 PM
Then we wondered, how would this impact transcription, would it reflect gastruloid's temporality or morphology? In contrast with morphology, the emergence of a Mesp2 pole was incredibly tight between different sizes #NotintheGenes?