Thank you! 🧬🙏🏼

Thank you! We work with some really talented science artists and have avid cartoonists in the lab as well 😅

This work was driven by uniquely talented @humollidorentina.bsky.social with Jessica Ransome and @damienpiel.bsky.social. We worked together with Jan-Willem Veening @veeninglab.com for the data analysis strategy.

Hope you all like HIDEN-SEQ and will use it for cool discoveries! 8/8

Is it all about bacterial immunity? No! Bas54 and its relatives (including also iconic phi92) have a "Swiss Army knife" of tail fibers of which different ones seem to bind diverse receptors on different host strains.

7/8

Intriguingly, highly specific sets of viral genes are conditionally essential for each phage on different uropathogenic Escherichia coli strains (obtained from the @nccr-antiresist.bsky.social consortium). We show that many of them are new anti-defense factors.

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We also applied HIDEN-SEQ to other phages such as T-even phage Bas37 and (shown below) Bas54 of the abundant but understudied Vequintavirinae. These results will kickstart studies on the fascinating molecular biology of these phages.

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It took generations of scientists to get a basic idea about gene essentiality and (at least for half of them) gene function in model phage T4. HIDEN-SEQ can generate the same data across the genome at near-basepair resolution in one shot.

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Read all details in our preprint - a massive breakthrough driven by ingenuity and persistence of rockstar PhD student Dorentina Humolli @humollidorentina.bsky.social. This is also the first major work since our lab moved to @ethz.ch Zürich D-HEST.
biorxiv.org/content/10.1...

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First author Dorentina Humolli @humollidorentina.bsky.social has made a fantastic thread about the details: bsky.app/profile/humo...

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