Achilles Kapanidis
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achilleskap.bsky.social
Achilles Kapanidis
@achilleskap.bsky.social
150 followers 85 following 11 posts
Professor of Biological Physics at Oxford.
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achilleskap.bsky.social
First BlueSky post + a breath of fresh air! Excited about our preprint on the packaging + structure of viral RNA segments in influenza virions: doi.org/10.1101/2025.... A saga from amazing Christof and Qing, and a great collaboration with @nicolerobb.bsky.social and @efodor.bsky.social.
A DNA-PAINT protocol for the segment-specific detection of viral vRNPs. Virus particles are surface-immobilized, fixed, permeabilized and incubated with non-fluorescent barcoded probes, that bind to 20-50 different sequences on a viral RNA segment. All probes carry the same segment-specific oligonucleotide extension that is transiently bound by fluorescent imager strands. Several combinations of barcode extensions and imager strands can be used to sequentially to detect various gene segments in a single virus particle.
February 17, 2025 at 3:07 PM