With Christmas just around the corner, it’s time for the #FluorescenceFriday post🎄!

This week, we’re featuring a hiPSC reporter line engineered to express 2 fluorescent proteins—located in the nuclei and actin filaments of #hiPSCs, generated using STRAIGHT-IN Dual🧪🔬

@benjaminbjohnson.bsky.social?

Same, but in 3D cardiac organoids 🚦🔴

Calcium signals in beating hiPSCs-derived cardiomyocytes.
A single copy of this reporter was integrated using the STRAIGHT-IN platform at the AAVS1 locus.
doi.org/10.1016/j.cr...
doi.org/10.1101/2024...

Here is my 2nd #FluorescenceFriday post! 🧪 #hiPSCs expressing jRCaMP1b, a genetically-encoded Ca2+ indicator 👻. This reporter allows us to record intracellular Ca2+ in hiPSC-derived #cardiomyocytes and cardioids (videos in the comments) #microscopy 🔬 #Science #Biology @benjaminbjohnson.bsky.social

Hey #BlueSky! Here is my first #FluorescenceFriday post!
This cardiac organoid was derived from #hiPSC and imaged using light sheet #microscopy 🔬.

🔵 Cardiomyocytes
🔴 Endothelial cells
🟡 Fibroblasts

More coming next week! 🧪

#Science #Biology #CellBiology #SciArt
@benjaminbjohnson.bsky.social