#wetlab folks, looking for an alternative to 0.15% saponin for selective lysis of erythrocytes, any advice?

shoutout to @atinygreencell.bsky.social for the workflow hacks

looking for decent books or texs that covers the phylogeny of protists and bonus points for taxonomic changes through history

any protistology stuff would be cool too, I'm especially interested in stentors

looking for books or resources that might cover something along the lines of emergency rural medicine know-how, something a little more comprehensive than field/survival first-aid tutorials

on keeping notes, I am reminded of Bialek's recounting what Kittel said in his stat mech. class, "the exercise of deciding what you think belongs on that piece of paper is a very effective way of studying"

granted he was talking about open book testing, but the point stands I think

Looking to extract DNA from a gel, no gel extraction kits though so I'm going to try the freeze & squeeze protocol, but not quite sure what kind of synthetic fiber I can use in the initial 0.5ml tube, any tips?