Please reach out if you/your trainees have any questions. And please apply!

As someone who started here in 2020, I feel lucky that we will be able to hire this cycle since I know how nerve-wracking the faculty job search can be, especially in years where external factors make the process even less predictable than usual.

At Dartmouth Biology we have a great graduate program in molecular and cell biology (@mcbdartmouth.bsky.social), a kind and supportive departmental culture, and solid core support all set in the bucolic Connecticut river valley in Hanover, NH.

Don't hesitate to contact me with any questions.

Apply here: searchjobs.dartmouth.edu/postings/772...

Lab website: sites.dartmouth.edu/amodeolab/

Link to core director position:
apply.interfolio.com/154316

Link to Protein Biochemist – Research Scientist position:
apply.interfolio.com/161499

These results surprised us and have us thinking about zygotic chromatin composition in totally new ways! As always, biology is way more interesting and complicated than out overly simplistic initial explanations.

Finally we used Chk1 to prevent cell cycle slowing and that did decrease H3.3 chromatin incorporation. BUT, it wasn't due to the cell cycle since H3.3 decrease precedes the change in cell cycle length!

Next we thought that maybe H3.3 incorporation was downstream of transcription, but when she knocked down Zelda H3.3 was still incorporated just fine.

In the first version of this paper we proposed that this was due to exhaustion of the (prefered) maternal H3 supply and that H3.3 was incorporated as a "second choice" H3. But when Anusha actually reduced the level of H3... H3.3 incorporation was unaffected!

We made made H3/H3.3 chimeras and saw that chaperone binding sites were key. Also, H3/H3.3 chromatin levels depend on the local Nuclear to cytoplasmic ratio. But how do histones know the N/C ratio.

We started out trying to explain why H3 goes up and H3.3 goes down on chromatin leading up to ZGA in the fly embryo. This is interesting because H3.3 is associated with both active transcription and heterochromatin which appear at ZGA.