Shazeb Ahmad
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shazebahmad.bsky.social
Shazeb Ahmad
@shazebahmad.bsky.social
Passionate about exploring RNA viral evolution and host-pathogen interactions. #Phages #RNAPhages #RNAviruses
Excited to share my first-author publication from my #PhD work at @helmholtz-hiri.bsky.social 🎉

We established mudRapp-seq, a spatial transcriptomic technique that combines direct RNA padlock probing and in-situ sequencing.

academic.oup.com/nar/article/...
Visualizing the transcription and replication of influenza A viral RNAs in cells by multiple direct RNA padlock probing and in situ sequencing (mudRapp-seq)
Abstract. Influenza A viruses (IAVs) contain eight negative-sense single-stranded viral RNA (vRNA) molecules, which are transcribed into messenger RNA (mRN
academic.oup.com
June 11, 2025 at 1:45 PM
Reposted by Shazeb Ahmad
If you want to do single-cell RNA-seq on your favourite bacterium, here‘s our detailed Nature Protocol for microbial MATQ-seq. It profiles up to 600 genes in a single bacterial cell!

www.nature.com/articles/s41...
Transcriptomic profiling of individual bacteria by MATQ-seq - Nature Protocols
Bacterial single-cell sequencing via MATQ-seq combines index sorting, random priming and rRNA depletion, and offers high cell retention and transcript capture rates, making it ideal for experiments wi...
www.nature.com
April 9, 2025 at 9:15 AM
Reposted by Shazeb Ahmad
Phage coexistence, even when you least expect it.

Check out @norapyenson.bsky.social's study showing the repeated assembly of diverse phage communities: www.science.org/doi/10.1126/...

Also Nora's bsky thread here: bsky.app/profile/nora...

#SocialViruses #virosky #evosky 🧪🦠
December 19, 2024 at 12:25 PM