Roni Miah
@ronimiah.bsky.social
Applied Molecular Microbiologist
Postdoc, HMI research group, UiT, Tromsø, Norway
Postdoc, HMI research group, UiT, Tromsø, Norway
Happy to share our recently accepted research article in iScience(Cell Press). Indeed it was an excellent team work. Special thanks to project PI @christianslentz.bsky.social, co-PI @monaj66.bsky.social and excellent CRISPRi collaborator @mkjos.bsky.social. Wish to learn many more with this tool!
A programmable, selection-free CRISPR interference system in Staphylococcus aureus for long-term host interaction studies
Common dCas9-based CRISPR interference (CRISPRi) systems for manipulating bacterial gene expression require antibiotic selection and exogenous inducer molecules, limiting their applicability in infection models. For Staphylococcus aureus, we have developed a programmable, selection-free CRISPRi system leveraging the pCM29 plasmid, which is stable without antibiotic selection. In this system, dCas9 expression is regulated by an endogenous promoter, and sgRNA expression is driven by a constitutive promoter, eliminating the need for exogenous inducer molecules.
www.cell.com
August 26, 2025 at 3:21 PM
Happy to share our recently accepted research article in iScience(Cell Press). Indeed it was an excellent team work. Special thanks to project PI @christianslentz.bsky.social, co-PI @monaj66.bsky.social and excellent CRISPRi collaborator @mkjos.bsky.social. Wish to learn many more with this tool!
Excellent talk by @christianslentz.bsky.social outlining ABPP as a promising approach for screening potential drug targets of pathogenic bacteria in context of host cell.
April 14, 2025 at 9:01 PM
Excellent talk by @christianslentz.bsky.social outlining ABPP as a promising approach for screening potential drug targets of pathogenic bacteria in context of host cell.
Disclosing my developed CRISPRi system in a global platform!. Grateful to the ESCMID committee for awarding me travel grant, registration fee waiver, and additional support. Special thanks to @christianslentz.bsky.social, @monaj66.bsky.social , @mkjos.bsky.social.
April 13, 2025 at 9:59 PM
Disclosing my developed CRISPRi system in a global platform!. Grateful to the ESCMID committee for awarding me travel grant, registration fee waiver, and additional support. Special thanks to @christianslentz.bsky.social, @monaj66.bsky.social , @mkjos.bsky.social.
Reposted by Roni Miah
Preprint alert from the lab! Exciting new work led by @ronimiah.bsky.social Introducing a programmable, selection-free CRISPRi system in S. aureus.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
A programmable, selection-free CRISPR interference system in Staphylococcus aureus
Common dCas9-based CRISPR interference (CRISPRi) system for gene regulation requires antibiotic selection and exogenous inducer molecules, posing significant challenges when applied in in vivo bacteri...
www.biorxiv.org
December 9, 2024 at 12:38 PM
Preprint alert from the lab! Exciting new work led by @ronimiah.bsky.social Introducing a programmable, selection-free CRISPRi system in S. aureus.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Roni Miah
A programmable, selection-free CRISPR interference system in Staphylococcus aureus https://www.biorxiv.org/content/10.1101/2024.11.27.625599v1
A programmable, selection-free CRISPR interference system in Staphylococcus aureus https://www.biorxiv.org/content/10.1101/2024.11.27.625599v1
Common dCas9-based CRISPR interference (CRISPRi) system for gene regulation requires antibiotic sele
www.biorxiv.org
November 28, 2024 at 2:17 AM
A programmable, selection-free CRISPR interference system in Staphylococcus aureus https://www.biorxiv.org/content/10.1101/2024.11.27.625599v1