AbstractObjectives. The study investigated the knowledge and awareness of anti-doping regulations among community pharmacists in Norway.Methods. The resear

Salmeterol is a commonly used β2-agonist included on the List of Prohibited Substances and Methods published by the World Anti-Doping Agency (WADA). We developed a population pharmacokinetic (popPK) ...

A large number of small peptides are extracted from both cellulose and polymeric DBS supports and analysed by UHPLC–HRMS. Targeting the 13C isotopologues in full scan or using MS/MS acquisition mode ...

The migraine medicine, Migsis, 5 mg, was administered to females (n = 8) and males (n = 4). Lomerizine M6 was useful in urine from both males and females to determine the origin of trimetazidine. The...

0.75x 1x 1.25x 1.5x 2x 0:0050:38 167 – Supporting Anti-Doping and Changing the Culture of Youth Sport Through TrueSport – Jennifer Royer, PhD Apple PodcastsGoogle PodcastsPlayer EmbedShare Leave a Rev...

RYR-stabilizers such as S107, JTV-519, ARM 036, and ARM 210 are emerging doping-relevant compounds due to their performance-enhancing effects on leaky skeletal muscle Ca2+ channels. To support proact...

Dexamethasone (DEX) is prohibited in sports competitions when administered by all injectable, oral or rectal routes, and is permitted at all times whe…

Doping in sport remains a persistent global challenge, eroding sporting integrity, athlete welfare, and public trust. Over the past 25 years, psycholo…

Tetrahydromethyltestosterone (THMT) and 20-hydroxymethyl-18-nortetrahydromethyltestosterone (20OHnorTHMT) are metabolites of the anabolic androgenic steroids methyltestosterone and metandienone. Both....

The current limited understanding of small bioactive peptide metabolism hinders effective doping control. A primary challenge lies in distinguishing suspicious features from extensive mass spectral datasets contaminated by biological matrix interference and background noise. In this study, we introduce a strategy integrating in silico prediction and nontargeted data mining to achieve more comprehensive metabolic profiling through ultrahigh-performance liquid chromatography–high-resolution mass spectrometry (UHPLC-HRMS). The strategy operates by identifying and applying chemical similarity (CSIM) rules of peptides (such as LC/MS behaviors and specific biotransformation) to mine unknown metabolites. With this strategy, a semiautomated workflow utilizing computational software and custom script was constructed and applied to the human liver microsomal metabolism of two significant kisspeptin analogues with doping potential (TAK-448 and TAK-683). The characteristic behaviors induced by CSIM enabled effectively in-depth data mining from redundant background signals, leading to the identification of 13 metabolites (three were validated via synthetic standards) and two uncommon biotransformation pathways (N-terminal vinylation and N-terminal carboxylation) for both investigated compounds. Notably, the two biotransformation pathways offered an innovative perspective on small peptide metabolism, which was further confirmed in rats, and produced two promising long-term metabolites for monitoring doping abuse. Further drug activity evaluation indicated higher or retained performance-enhancing effects of these metabolites, alerting doping control subjects to pay attention to more information. The study provided the first comprehensive characterization of the metabolic profiles of TAK-448 and TAK-683, while also offering an effective tool for metabolism research on small peptide doping agents.

0.75x 1x 1.25x 1.5x 2x 0:0039:54 166 – Bringing the Athlete Perspective to Elite Anti-Doping Education – Greta Neimanas, PLY Apple PodcastsGoogle PodcastsPlayer EmbedShare Leave a ReviewListen in a Ne...

Optimal detection of Erythropoietin receptor agonists (ERAs) in anti-doping analyses relies heavily on efficient immunopurification (IP) strategies. W…

This study shows that clenbuterol, a potent and heavily abused β2-agonist, induces KLHL41 expression—a sarcomeric myofibrillogenesis protein—in human skeletal muscle comparably to resistance training...

Among the phase-I reactions of 6-chloro-testosterone (6-CT), the reductive and oxidative dehalogenation, as well as the dehydrogenation, have been des…