Mike Hough
@mikehough.bsky.social
Structural biologist and beamline scientist at Diamond light source, Hon Prof @ University of Essex. Interested in room temperature macromolecular crystallography, serial and time resolved work - and metalloproteins.
Recent paper showing the power of combining advanced structural biology with QM/MM simulations to understand a heme enzyme. Work between Diamond, STFC, Bristol and Essex. We present evidence for the presence of an unusual protein to heme cross-link.
pubs.rsc.org/en/content/a...
pubs.rsc.org/en/content/a...
pubs.rsc.org
August 22, 2025 at 2:00 PM
Recent paper showing the power of combining advanced structural biology with QM/MM simulations to understand a heme enzyme. Work between Diamond, STFC, Bristol and Essex. We present evidence for the presence of an unusual protein to heme cross-link.
pubs.rsc.org/en/content/a...
pubs.rsc.org/en/content/a...
New paper describing use of photocaged nitric oxide for time resolved crystallography within fixed targets at XFEL and synchrotron - PhD work of Pete Smyth & Lewis Williams - great collaboration with @jarworrall.bsky.social, beamline I24 and SACLA. Funded by BBSRC
journals.iucr.org/m/issues/202...
journals.iucr.org/m/issues/202...
Time-resolved serial synchrotron and serial femtosecond crystallography of heme proteins using photocaged nitric oxide
We demonstrate the application of a nitric oxide releasing photocage system for time-resolved serial crystallography studies of two heme containing proteins using a fixed target sample delivery system...
journals.iucr.org
August 22, 2025 at 12:52 PM
New paper describing use of photocaged nitric oxide for time resolved crystallography within fixed targets at XFEL and synchrotron - PhD work of Pete Smyth & Lewis Williams - great collaboration with @jarworrall.bsky.social, beamline I24 and SACLA. Funded by BBSRC
journals.iucr.org/m/issues/202...
journals.iucr.org/m/issues/202...
Paper describing clustering methods to separate subtle structural differences in populations of protein crystals - with application to polymorphs, ligand binding.. - led by Amy Thompson @amyjayyyne.bsky.social with VMXi,I24 and Data Analysis at @ddiamond.bsky.social journals.iucr.org/d/issues/202...
Enhanced intensity-based clustering of isomorphous multi-crystal data sets in the presence of subtle variations
Bovine, porcine and human insulin data sets are isomorphous in terms of unit cell and symmetry. Through enhancements to the multi-crystal clustering methods in DIALS, species-pure data sets are automa...
journals.iucr.org
June 2, 2025 at 7:41 AM
Paper describing clustering methods to separate subtle structural differences in populations of protein crystals - with application to polymorphs, ligand binding.. - led by Amy Thompson @amyjayyyne.bsky.social with VMXi,I24 and Data Analysis at @ddiamond.bsky.social journals.iucr.org/d/issues/202...
Our new paper exploring the effect of XFEL pulse length and pulse intensity on electron density maps of two radiation sensitive proteins. A great collaboration between Diamond, SwissFEL and University of Essex's Worrall group.
journals.iucr.org/m/issues/202...
journals.iucr.org/m/issues/202...
Damage before destruction? X-ray-induced changes in single-pulse serial femtosecond crystallography
Varied pulse-duration and pulse-intensity serial femtosecond crystallography data do not show significant signs of radiation damage under typical experimental conditions.
journals.iucr.org
April 15, 2025 at 12:19 PM
Our new paper exploring the effect of XFEL pulse length and pulse intensity on electron density maps of two radiation sensitive proteins. A great collaboration between Diamond, SwissFEL and University of Essex's Worrall group.
journals.iucr.org/m/issues/202...
journals.iucr.org/m/issues/202...
Nice to see our review on the fascinating cytochromes P460 and cytochromes c' - that use a similar and unusual structure to carry out very different functions out in JBIC. An idea for a mini review that expanded! link.springer.com/article/10.1...
Cytochromes P460 and c′-β: exploiting a novel fold for multiple functions - JBIC Journal of Biological Inorganic Chemistry
Two related classes of ligand-binding heme c-containing proteins with a high degree of structural homology have been identified and characterized over recent decades: cytochromes P460 (cyts P460), def...
link.springer.com
March 4, 2025 at 12:09 PM
Nice to see our review on the fascinating cytochromes P460 and cytochromes c' - that use a similar and unusual structure to carry out very different functions out in JBIC. An idea for a mini review that expanded! link.springer.com/article/10.1...
Just getting started on blue sky - Using this mostly for work purposes, as a brief intro for people I don't already know - I'm a structural biologist at Diamond Light Source specialising in room temperature X-ray structures and serial crystallography at synchrotrons and XFELs
November 26, 2024 at 8:58 AM
Just getting started on blue sky - Using this mostly for work purposes, as a brief intro for people I don't already know - I'm a structural biologist at Diamond Light Source specialising in room temperature X-ray structures and serial crystallography at synchrotrons and XFELs