Metabarcoding and Metagenomics
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Metabarcoding and Metagenomics
@mbmg.pensoft.net
Open-access, peer-reviewed journal for metabarcoding & metagenomics research.

Welcomes submissions documenting all types of outcomes throughout the research cycle.

Published by @pensoft.net. Powered by ARPHA platform.
Website: mbmg.pensoft.net.
Reposted by Metabarcoding and Metagenomics
As 2025 draws to a close, we at Pensoft want to extend our warmest wishes to our editors, reviewers, researchers, partners, and followers. Thank you for being part of our journey this year.

Wishing you all a joyful holiday season and a bright start to the new year! 🎄✨
December 23, 2025 at 9:11 AM
Reposted by Metabarcoding and Metagenomics
Day three of #BES2025 is underway! Find us at booth L12 in the Lennox Suite.
We are delighted to be exhibiting at #BES2025 this week! Head to the Lennox Suite (L12) to say hello and learn about our open-access journals.
December 17, 2025 at 9:49 AM
This baseline study explores meiofauna diversity in Baltic Sea marine protected areas and adjacent reference areas using multi-gene metabarcoding and morphology - key for tracking trawling ban impacts​.

🔗 doi.org/10.3897/mbmg...

@sgn.one
Baseline studies on meiofauna in the Baltic Sea before bottom-trawl fisheries exclusion II. A comparative study using multi-gene metabarcoding and morphology
Understanding how meiofaunal communities respond to spatial protection measures is essential for evaluating the effectiveness of marine conservation strategies, particularly in heavily impacted region...
doi.org
December 12, 2025 at 12:56 PM
Across 21 European lakes, metagenomes and metatranscriptomes reveal different, method-dependent pictures of microbial diversity and environment links, proving that sequencing choice can alter ecological conclusions.

🔗 doi.org/10.3897/mbmg...

@unidue.bsky.social
Constraining lake ecology via metagenomics, metatranscriptomics, and amplicon sequencing
Lake ecosystems are hotspots for important ecosystem functions, yet linking their microbiome to physicochemical parameters remains a challenge. Here, we compare 16S rRNA gene-based amplicon sequencing...
doi.org
December 4, 2025 at 12:16 PM
Integrating legume-based green manure and organic amendments in barley cropping systems enhances rhizosphere microbial diversity and resilience, promoting sustainable soil health and productivity.

🔗 doi.org/10.3897/mbmg...
Barley Rhizosphere Bacteriome Dynamics under Organic and Mineral Inputs: The importance of intercropping predecessor
Integrating intercropping with green manure within crop rotation offers a sustainable pathway to enhance soil microbial diversity and overall agroecosystem health. This study evaluated the effects of ...
doi.org
November 19, 2025 at 11:49 AM
Reposted by Metabarcoding and Metagenomics
Come to the Pensoft stand at the Kliments' Days 2025 in Sofia, Bulgaria! Our Marketing and Sales manager @maria-kolesnikova.bsky.social is there to welcome you and tell you all about our open-access journals in biodiversity sciences!
November 6, 2025 at 8:22 AM
A targeted DNA metabarcoding workflow was developed and validated to identify terrestrial gastropods from the digestive contents of the invasive flatworm 𝑂𝑏𝑎𝑚𝑎 𝑛𝑢𝑛𝑔𝑎𝑟𝑎, revealing diverse prey and improving diet analysis.

🔗 doi.org/10.3897/mbmg...
A DNA metabarcoding workflow for the detection of terrestrial gastropods from ingested DNA
DNA metabarcoding is a powerful approach for assessing biodiversity and unravelling trophic interactions, particularly in the context of biological invasions where introduced predators may impact nati...
doi.org
November 6, 2025 at 7:30 AM
eDNA metabarcoding outperforms traditional electrofishing for monitoring fish and decapod biodiversity in Caribbean freshwater ecosystems, offering a less disruptive, more comprehensive method to detect native and invasive species and assess ecological health.

🔗 doi.org/10.3897/mbmg...
Metabarcoding outperforms traditional electrofishing in decapod and fish inventories, paving the way for enhanced biodiversity monitoring in the Caribbean
Environmental DNA (eDNA) metabarcoding revolutionized the biodiversity monitoring in aquatic ecosystems, giving access to taxonomic lists in a non-disruptive way. Although the method has limits, such ...
doi.org
November 4, 2025 at 8:09 AM
Reposted by Metabarcoding and Metagenomics
Reposted by Metabarcoding and Metagenomics
DNA barcoding reference dataset revealed overlooked diversity and clarified biogeography of fishes in the largest European river system – Volga River.

🔗 doi.org/10.3897/arph...

@mbmg.pensoft.net
DNA barcoding reference dataset revealed overlooked diversity and clarified biogeography of fishes in the largest European river system – Volga River
This study represents the first thorough genetic assessment of freshwater fishes from the Volga River basin – the longest (3,690 km) river in Europe. DNA barcode sequences (COI) were studied in ...
doi.org
October 31, 2025 at 1:42 PM
Our journal is at #LivingData2025 - looking forward to talking to anyone interested in #metabarcoding and metagenomics!
We're here, #LivingData2025!

Come visit us at booth 9 and our team, headed by Pensoft CEO Prof. Lyubomir Penev, will tell you all about our involvement in biodiversity-related #HorizonEU & #H2020 project as well as our commitment to #openaccess publishing!
October 24, 2025 at 10:27 AM
Taxon-specific mini-barcode primers greatly improve the detection and biodiversity assessment of key marine zooplankton groups, boosting species representation by up to fivefold.

🔗 doi.org/10.3897/mbmg...
The design and testing of taxon-specific mini-barcode markers for metabarcoding of marine zooplankton
DNA metabarcoding of zooplankton samples is well suited to surveys of marine pelagic ecosystems; however, consistent amplification across zooplankton groups cannot be assumed when using “universal” mi...
doi.org
October 24, 2025 at 8:27 AM
Reposted by Metabarcoding and Metagenomics
Guidance and best practices for species identification using eDNA metabarcoding - When do you call a cod a cod?

🔗 doi.org/10.3897/arph...

@mbmg.pensoft.net
Guidance and best practices for species identification using eDNA metabarcoding - When do you call a cod a cod?
With the rapid uptake of environmental DNA (eDNA) metabarcoding for a wide range of conservation and management uses, there is a growing need for guidance and best practices for species identification...
doi.org
October 24, 2025 at 6:31 AM
Multiplex PCR sequencing represents a powerful and cost-effective tool for simultaneously detecting multiple aquatic invasive species in the early stages of invasion: doi.org/10.3897/mbmg...
Invasive species monitoring based on eDNA multiplex PCR sequencing
Invasive species pose a significant threat to global biodiversity and the stability of ecosystems. Although environmental DNA (eDNA)-based quantitative PCR is considered effective, its limited multipl...
doi.org
October 15, 2025 at 6:09 AM
"Want to start using Nanopore technology to research protistan diversity? Check out our paper introducing a pipeline for creating OTUs from Nanopore metabarcodes — bridging the gap between short- and long-read metabarcoding." - Anna Karnkowska

doi.org/10.3897/mbmg...
October 14, 2025 at 10:16 AM
Non-invasive swabbing of hive entrances is a highly effective biomonitoring method for collecting hive eNA and delivering comprehensive surveillance for animal, plant and microbial communities in the environment.

🔗 doi.org/10.3897/mbmg...
Surface swabs outperform most traditional honeybee (Apis mellifera) hive samples for recovery of eDNA and eRNA
Genetic sampling from European honeybee (Apis mellifera) hives is a promising repository of terrestrial environmental nucleic acids (eNA) from a range of taxonomic sources. eNA samples from honeybee h...
doi.org
October 13, 2025 at 11:29 AM
Reposted by Metabarcoding and Metagenomics
Optimised eDNA and PCR-based metabarcoding significantly outperformed hybridisation capture and traditional field surveys for bryophyte detection in river ecosystems.

🔗 doi.org/10.3897/mbmg...
A comparative analysis of hybridisation capture and PCR-based eDNA metabarcoding for monitoring bryophytes in riparian ecosystems
Despite their ecological importance, mosses remain under-represented in ecological studies due to their challenging detection in field surveys and morphological identification, exacerbated by the lack...
doi.org
October 10, 2025 at 11:31 AM
Researchers present the BaNaNA (Barcoding Nanopore Neat Annotator) pipeline and display the effectiveness of long-read Nanopore sequencing for protist biodiversity, ecology and evolution research.

🔗 doi.org/10.3897/mbmg...

@ankarn.bsky.social @fabnot.bsky.social
From short to long reads: enhanced protist diversity profiling via Nanopore metabarcoding
In the last decades, environmental metabarcoding has revolutionised biodiversity research, particularly for microbial organisms such as protists, enabling large-scale assessments of diversity and ecol...
doi.org
October 8, 2025 at 1:27 PM
DNA metabarcoding identifies more invertebrate species under the Water Framework Directive than conventional methods, showing promise for faster and finer river health monitoring in Slovakia’s Carpathian streams.

🔗 doi.org/10.3897/mbmg...
Evaluating the potential of DNA metabarcoding for ecological status assessment under the Water Framework Directive: A case study on benthic invertebrates from Western Carpathian streams
Routine biomonitoring under the Water Framework Directive (WFD) follows a conventional methodology primarily based on the morphological identification of taxa within the five biological quality elemen...
doi.org
October 3, 2025 at 7:22 AM
Tiina Laamanen @tiinalaamanen.bsky.social cited her MBMG paper on molecular biodiversity monitoring methods as part of her #BGEconnects session on implementing #eDNA and other molecular monitoring methods in Finland!
September 30, 2025 at 4:51 PM
Reposted by Metabarcoding and Metagenomics
After more than fourteen years of advancing research on biological invasions, we have published our 100th issue! 🌎

🔗 blog.pensoft.net/2025/09/29/a...
Advancing invasion science: NeoBiota publishes its 100th issue
The open-access journal NeoBiota has published its milestone 100th issue, celebrating more than fourteen years of advancing research in invasion science.
blog.pensoft.net
September 29, 2025 at 10:59 AM
Reposted by Metabarcoding and Metagenomics
DNA barcoding reveals that the diversity of 𝑁𝑖𝑝ℎ𝑎𝑟𝑔𝑢𝑠 in France is dramatically underestimated.

🔗 doi.org/10.3897/zse....
Diversity of Niphargus (Crustacea, Amphipoda) in France, as revealed by DNA barcoding approach
Niphargus is the most speciose amphipod genus inhabiting groundwater of the Western Palearctic. The genus is renowned for its notorious taxonomy, associated with large within- but often negligible bet...
doi.org
September 25, 2025 at 11:50 AM
Reposted by Metabarcoding and Metagenomics
📰 A recent article, supported by our project, was published in Pensoft Publishers' (@pensoft.net) Metabarcoding and Metagenomics journal.

🧬 The study evaluates the TRLs of molecular tools for #monitoring like #eDNA and DNA #metabarcoding.

👀 Full article:
mbmg.pensoft.net/article/1308...
September 19, 2025 at 10:37 AM
Reposted by Metabarcoding and Metagenomics
The latest guest post on the @pensoft.net blog outlines a new, non-lethal method of studying pollinator networks. 🐝🧬

blog.pensoft.net/2025/09/11/t...
The bee’s knees: a new, non-lethal way to study pollinator networks
Researchers have created a new way to study pollinator networks, using metabarcoding and without killing bees, as described in this guest blog post.
blog.pensoft.net
September 11, 2025 at 9:52 AM