The first results of using a new type of ultrahigh-resolution mass analyzer based on a planar multipass time-of-flight mass spectrometer with periodic reflecting lenses (Y-MRT MS) for bottom-up whole-...

Glycosylation, the enzymatic addition of carbohydrate moieties to proteins, is essential for immune recognition, protein folding, and disease progression. The structural complexity of glycans and the...

Rationale Proton transfer reaction mass spectrometry (PTR-MS) is a widely used technique for on-line detection of volatile organic compounds (VOCs). Sensitivity enhancement is an important developme.....

Mass spectrometry instrumentation continues to evolve rapidly, yet quantifying these advances beyond conventional peptide and protein detections remains challenging. Here, we evaluate a modified Orbit...

Cyclic ion mobility spectrometry (cIMS) provides the potential for high resolution separations of small molecule isomers using multiple passes in a closed-loop geometry. Achieving this potential, howe...

An open-source platform for data-independent acquisition proteomics adapts predicted libraries to experimental settings.

Limited sensitivity and depth of proteome sampling in experiments using data-dependent acquisition (DDA) mass spectrometry are usually attributed to an insufficient rate of fragmentation spectra acquisition relative to the number of coeluting potential targets. Here, we demonstrated that limited sensitivity and dynamic range of MS1 scans reduce detection of low-intensity ions and thus their selection for fragmentation. As abundant ions occupy a large fraction of the ion accumulation capacity, we sought to improve MS1 detection of rare analytes by an easily implementable strategy based on gas-phase segmentation of the MS1 scan range, followed by coaccumulation and detection of all ions. The quadrupolar isolation windows used to segment the MS1 scan range are designed to transmit, on average, an equal number of charges, consistent with the parameter used by many recent mass spectrometers to regulate ion trap filling. This strategy, which we named high dynamic range MS1 (HDR-MS1), reduces the contribution of abundant ions to reaching the maximum ion capacity. As a result, HDR MS1 showed improved dynamic range and sensitivity compared to conventional full-range scans, resulting in a higher number of peptides and protein identifications under identical MS2 parameters, less redundant precursor ion sampling, and a higher rate of quantified precursor ions. HDR MS1 scans are compatible with any DDA precursor selection filter and MS2 parameter, and the generated files can be analyzed using any software for peptide-spectral matching and quantification.

There are many potential advantages to performing ion manipulations at atmospheric pressure (AP) compared to in vacuum. However, the RF electric fields commonly employed in vacuum-based systems are mo...

A quadrupole/drift tube ion mobility/Orbitrap instrument requires pumping from atmosphere to 10–5 Torr in the quadrupole analyzer region, back to 1 Torr for the drift tube, and a return to 10–5 Torr f...

The sensitivity of the atmospheric pressure ionization mass spectrometer (API-MS) has been improved owing to the advancement in the atmospheric pressure interface. However, ion scattering losses in th...

The inherent heterogeneity of biological macromolecules offers a unique challenge for analysis. The combination of ion mobility (IM) and mass spectrometry (MS) is sensitive to the size, shape, and dyn...

Applications using real-time spectral analysis and real-time instrument control have emerged in recent years as powerful tools to improve the capabilities and sensitivity of mass spectrometers. Softwa...

Orbitrap (OT)-based mass spectrometer platforms are a gold standard in high-resolution mass spectrometry, where their primary disadvantage is slower-scanning speed in comparison to time-of-flight or l...

In charge detection mass spectrometry (CD-MS) ions are trapped in an electrostatic linear ion trap (ELIT) where they oscillate back and forth through a conducting cylinder. The oscillating ions induce...

In the last few years, high-resolution time-of-flight mass analyzers (TOF-MA) have been intensively developed. Their development requires tools that p…

The coupling of electrospray ionization (ESI) with the newly discovered acoustic ion manipulation (AIM) phenomenon is demonstrated for biopolymer analytes, including peptides, proteins, and DNA. It is...

Two-dimensional tandem mass spectrometry (2D-MS/MS) is a powerful analysis technique which can quickly produce a map of all precursor-product ion rela…

Publication date: Available online 26 August 2025 Source: International Journal of Mass Spectrometry Author(s): Charles A. Wolfe, Sydney Povilaitis, Michael F. Keating, Ashley E. Montgomery, Bryan M. Burt, Amir A. Jazaeri, Livia S. Eberlin

An electrostatic linear ion trap (ELIT) is used to trap ions between two ion mirrors with image current detection by central detection electrode. Tran…

Rationale Miniature mass spectrometers are increasingly used in portable and on-site analysis due to their compact size and light weight. However, miniaturization, higher operating gas pressure, and...