Lothar Schermelleh
@lschermelleh.bsky.social
Nuclear cell biologist with a passion for super-resolution imaging
Hot off the press: more reflections on the age-old discussion on how to think about optical resolution, particularly when it comes to biological application
journals.biologists.com/jcs/article/...
journals.biologists.com/jcs/article/...
Resolution in super-resolution microscopy – facts, artifacts, technological advancements and biological applications
Summary: Super-resolution microscopy (SRM) has emerged as a powerful tool for biological discovery. This paper compiles expert opinions on crucial, yet often overlooked, aspects of SRM that are essent...
journals.biologists.com
May 29, 2025 at 3:25 PM
Hot off the press: more reflections on the age-old discussion on how to think about optical resolution, particularly when it comes to biological application
journals.biologists.com/jcs/article/...
journals.biologists.com/jcs/article/...
All you nuclear biologists out there: Here is a cautionary tale not to be fooled by peripheral organelle staining. It might just be that your antibody got trapped because it is "too good".
🚨 Preprint alert 🚨
Excited to share our work on "Ab-trapping," an antibody artifact causing misleading peripheral ("rim") staining in imaging & genomics (IF, CUT&Tag, CUT&RUN). Antibodies fail to penetrate structures, accumulating at the periphery. A 🧵👇
doi.org/10.1101/2025...
Excited to share our work on "Ab-trapping," an antibody artifact causing misleading peripheral ("rim") staining in imaging & genomics (IF, CUT&Tag, CUT&RUN). Antibodies fail to penetrate structures, accumulating at the periphery. A 🧵👇
doi.org/10.1101/2025...
Ab-trapping - a peripheral staining artifact in antibody-based microscopy and genomics
Antibodies (Ab) are essential for detecting specific epitopes in microscopy and genomics, but can produce artifacts leading to erroneous interpretations. Here, we characterize a novel artifact, Ab-tra...
doi.org
April 16, 2025 at 5:15 PM
All you nuclear biologists out there: Here is a cautionary tale not to be fooled by peripheral organelle staining. It might just be that your antibody got trapped because it is "too good".