Deeply grateful to all contributors; Current or former #KaminskiLab members; generous collaborators: Takis, Ivan, Melanie, Jun, the FORMIDABLE @KU_Leuven
team - Wim & Bart; and special 👏🏽👏🏽 to #SuperDuo : Schupp & Adams‼️
Fin.
#SchuppAndAdams
#CureIPF
#ScienceMatters
www.biorxiv.org/content/10.1...

5/n Taken together, our results shift the paradigmatic view of alveolar epithelial cells in IPF from ‘innocent bystanders’ to 'drivers of disease' - maybe positioning Aberrant Basaloid Cells as potential targets for cell-based therapies
#CureIPF
#PrecisionMedicine
www.biorxiv.org/content/10.1...

@10xGenomics
Multiome analysis allowed us to establish Aberrant Basaloid Cells as a distinct lung cell population. It is different from all other lung cells and characterized by ⬆️⬆️ AP1 chromatin accessibility pattern reminiscent of Injury memory retention
#Multiome
www.biorxiv.org/content/bior...

3/n
To understand emergence of aberrant basaloid cells in human PCLS we did time course analysis.
Untreated PCLS -> ⬆️ alveolar epithelial diversity and proliferation suggesting
FC treated PCLS -> ⬇️ proliferation & disease like Aberrant Basaloid Cells
www.biorxiv.org/content/bior...

2/n
Using PAGA we define distinct trajectories among ATII, ATI, Aberrant basaloid cells & ATi cells that allow identifying specific markers for intermediate states & then validate and localize the presence of ATi populations in human IPF tissue
#CTSE
www.biorxiv.org/content/10.1...

1/n
Using snRNAseq we identify a previously undiscovered population of alveolar epithelial cells in IPF - Alveolar Intermediate (ATi) cells - they share features of ATI, ATII and Aberrant Basaloid cells but also have their own distinct features.
#ATi
#CureIPF
www.biorxiv.org/content/10.1...

Finally out as reprint!!!
"Alveolar epithelial cell plasticity & injury memory in human pulmonary fibrosis".
Glad to share the results of our single nuc-seq & single cell ATAC-seq analyses of human IPF & PCLS before peer review - see the key findings in 🧵👇🏽
www.biorxiv.org/content/10.1...