Jim Haseloff
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jimhaseloff.bsky.social
Jim Haseloff
@jimhaseloff.bsky.social
Plant biologist at the University of Cambridge. Mashing up low-cost DIY tools for bioinstrumentation (biomaker.org) and engineering plant biology (openplant.org)
Recipe for PPM (Plant Preservative Mixture) from the patent WO 1996/038542. Simplifies sterile plant tissue culture - especially if you don't have access to a research-grade facility. Great invention from Assaf Guri and Kishor Patel at Plant Cell Technology plantcelltechnology.com/blogs/blog/t...
November 4, 2025 at 5:46 PM
3D printed rotating tube mixer for incubating samples. Build instructions at: www.printables.com/model/52572-...
June 7, 2025 at 5:07 PM
Reposted by Jim Haseloff
Building on my PCB face plate idea, how about a PCB front and back (with the main board in between) and a TPU band round the edge hugging it all in place 🤔 this is just a basic test, but I think it might just work... #Pi0cket #CL32 #ESP32
April 26, 2025 at 8:03 PM
Reposted by Jim Haseloff
Excited to share the public release of the DIYNAFLUOR (DIY Nucleic Acid FLUORometer), a $40 USD, DIY, open-source, 3D-printed, solder-free, and portable DNA fluorometer you can build. 🦕 🧬

See our GitHub Repo for more! github.com/traulab/DIYN...

#DIYScience #STEM #DIYNAFLUOR
December 2, 2024 at 8:26 AM
Reposted by Jim Haseloff
Dear #DIYNAFLUOR users! Please check out the "experimental" folder on our github page.
tinyurl.com/4v23f629

We have a design revision that appears to improve the LoD by ~3.5x, providing quantitation down to 0.16 ng DNA. This should cover v.short nanopore library QC.

Would love any feedback! 🧪🧬🦕❤️
March 1, 2025 at 11:33 AM
Reposted by Jim Haseloff
Unlocking the power of Engineering Biology www.gov.uk/government/n...
Unlocking the power of Engineering Biology
New report outlines the transformative potential of Engineering Biology to tackle modern-day challenges.
www.gov.uk
April 25, 2025 at 3:58 AM
Reposted by Jim Haseloff
Semi-automated workflow for high-throughput Agrobacterium-mediated plant transformation. Summary of our efforts to accelerate plant transgenics with the @earlhaminst.bsky.social Biofoundry. Now published in @theplantjournal.bsky.social. #PlantScience #Synbio onlinelibrary.wiley.com/doi/10.1111/...
Semi‐automated workflow for high‐throughput Agrobacterium‐mediated plant transformation
A simplified and optimized pipeline for Agrobacterium tumefaciens mediated transformation of plants. Useful for obtaining stable transgenic lines of the model liverwort Marchantia polymorpha, or for ...
onlinelibrary.wiley.com
April 14, 2025 at 7:58 AM
Clever description of how to convert an inexpensive solar anchor buoy light into an extremely durable and waterproof solar powered Meshtastic node e.g. for sharing LoRa sensor data in a field setting. www.instructables.com/Meshtastic-S...
April 6, 2025 at 2:23 PM
Reposted by Jim Haseloff
1/6 🧵 Excited to share Pocket Spectrometer V2! After positive feedback on the first version of pocket spectrometer. I've made significant improvements (hardware+software) while keeping what worked well with the V1 #OpenSource #Spectrometer #STEM 💡
bsky.app/profile/chin...
March 9, 2025 at 11:35 PM
chinnadevarapu.bsky.social has updated his tiny, low cost Pocket Spectrometer design that uses an M5StickC microcontroller and AS7341 spectral sensor - simplified hardware connections and software improvements. Nice job! Details at: github.com/scientistnob...
March 9, 2025 at 6:47 PM
Reposted by Jim Haseloff
🚀✨ Exciting innovation alert! Meet the Pocket Spectrometer: a compact, open-source device 📏🔬 (< $50). Affordable, and portable, it is the open-source hardware for spectral analysis 🎨💡. Science in your pocket! 🌟👨‍🔬👩‍🔬 github.com/scientistnob...
GitHub - scientistnobee/Pocket-Spectrometer: I'm building what might be the smallest #opensource #spectrometer with an easy to use UI! I call it a pocket spectrometer. Combining #M5StickC (~$20) with ...
I&#39;m building what might be the smallest #opensource #spectrometer with an easy to use UI! I call it a pocket spectrometer. Combining #M5StickC (~$20) with AS7341 sensor (~$17.5). My goal? A com...
github.com
December 4, 2024 at 9:06 AM
The mini capsules can be opened and used for microscopic observation as well.
doi.org/10.1007/978-...
March 4, 2025 at 2:26 PM
Chopping tubes and adding slits to allow the halves to fit together...
doi.org/10.1007/978-...
March 4, 2025 at 2:25 PM
Handy little trick from Jose Ignacio Laines-Hidalgo, Angela F. Ku-Gonzalez, and Felipe A. Vazquez-Flota published in doi.org/10.1007/978-...
Making DIY small sealed capsules from Eppendorf tubes for plant histology.
March 4, 2025 at 2:24 PM
3D print files for a collection of parts that allow micro servos to be plugged into Fischertechnik construction systems. Good for prototyping programmable mechanical devices...
www.thingiverse.com/thing:2807112
January 2, 2025 at 6:23 PM
The described procedure for making Lenticules is relatively simple. Cells are mixed with a solution containing a high concentration of trehalose, BSA and carboxymethyl cellulose as caking agents, saline (with optional food dye), and dried on parafilm before storage over desiccant.
December 29, 2024 at 12:20 PM
However, details for preparation of Lenticules appears in a published patent: Storage of microorganisms, cells and tissue (WO 01/05941) See at: link.lens.org/aPFhU0Bjyli
December 29, 2024 at 12:14 PM
Lenticules were described in doi: 10.1046/j.1365-2672.1998.00611.x. by Codd et al. - but composition of the critical component for storage, the "lenticulating fluid (Codds Medium)" seems missing in this and a subsequent paper (dot: 10.1046/j.1365-2672.2001.01432.x)...
December 29, 2024 at 12:08 PM
Have always been interested in Lenticules and Vitroids (www.sigmaaldrich.com/deepweb/asse...) - as ways of storing/distributing microbial cells as stable, dry pellets, which could be widely useful for resource sharing. Lenticules originated at Public Health England, and a protocol is available...
December 29, 2024 at 11:55 AM
Wild collection of heirloom and genetically variant seeds available from Tomato Revolution (www.tomatorevolution.co.uk) ready for next summer.
December 27, 2024 at 12:13 PM
Reposted by Jim Haseloff
1/6 🧵 I'm building what might be the smallest #opensource #spectrometer with an easy to use UI! I call it a pocket spectrometer. Combining #M5StickC (~$20) with AS7341 sensor (~$16). My goal? A complete spectrometer that is barely larger than a sample cuvette itself.
November 25, 2024 at 11:36 PM
Reposted by Jim Haseloff
Downloadable reference card for focus bracketing, on a site full of examples and advice about Olympus macro photography systems from Chris McGinniss
www.chrismcginnis.com/gear
December 24, 2024 at 7:10 AM
Here's an archived free copy of CL Strong's 1960 book with collected Amateur Scientist columns from Scientific American. Inspiration for citizen science. Forward by Vannevar Bush...
archive.org/details/Proj...
December 24, 2024 at 6:26 AM
Online portfolio of bryophyte plant images from across the globe - excellent macrophotography from Des Callaghan, captures some of the morphological diversity of this clade
bryophytes.myportfolio.com/work
December 24, 2024 at 6:13 AM