Fred Hutch Innovation Lab
@hutchinnovation.bsky.social
FHIL is leading the in-house development and early adoption of powerful new single-cell and spatial technologies to spur discoveries in cancer immunotherapy.
Banking fixed samples before sorting improves experiment planning & instrument scheduling. 10x GEM-X DSP-based fixation recommends sorting before fixing cells. We attempted to stain, fix, and freeze cells before sorting & generating GEMs for 5' libraries. SFFS = STAIN FIX FREEZE SORT (1/7)
September 2, 2025 at 8:05 PM
Banking fixed samples before sorting improves experiment planning & instrument scheduling. 10x GEM-X DSP-based fixation recommends sorting before fixing cells. We attempted to stain, fix, and freeze cells before sorting & generating GEMs for 5' libraries. SFFS = STAIN FIX FREEZE SORT (1/7)
We offer the latest evaluation of 7 single-cell RNAseq technologies comparing recently updated chemistries to previous versions and across platforms in two separate experiments. Workflow, sensitivity, cost, sequencing efficiency, annotation, and DGE included. www.biorxiv.org/cgi/content/...
Evaluating the practical aspects and performance of commercial single-cell RNA sequencing technologies
The rapid development of updated and new commercially available single-cell transcriptomics platforms provides users with a range of experimental options. Cost, sensitivity, throughput, flexibility, a...
www.biorxiv.org
May 28, 2025 at 12:39 AM
We offer the latest evaluation of 7 single-cell RNAseq technologies comparing recently updated chemistries to previous versions and across platforms in two separate experiments. Workflow, sensitivity, cost, sequencing efficiency, annotation, and DGE included. www.biorxiv.org/cgi/content/...
Excited to share work from our team, released today. Check out how it compares to other methods on multiple platform's datasets. Would love to hear how it performs on your data.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Cell simulation as cell segmentation - Nature Methods
Proseg is a segmentation approach for single-cell spatially resolved transcriptomics data that uses unsupervised probabilistic modeling of the spatial distribution of transcripts to accurately segment...
www.nature.com
May 23, 2025 at 12:40 AM
Excited to share work from our team, released today. Check out how it compares to other methods on multiple platform's datasets. Would love to hear how it performs on your data.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
FHIL loves working with the Srivastava Lab! Their work explores factors enabling CAR-T response to checkpoint blockade in solid tumors. Infiltration of CAR-Ts was assessed spatially with a Xenium custom panel including probes targeting the construct and lung tumor. www.biorxiv.org/content/10.1...
www.biorxiv.org
April 24, 2025 at 5:56 PM
FHIL loves working with the Srivastava Lab! Their work explores factors enabling CAR-T response to checkpoint blockade in solid tumors. Infiltration of CAR-Ts was assessed spatially with a Xenium custom panel including probes targeting the construct and lung tumor. www.biorxiv.org/content/10.1...