Louise Johnson
@drlouisejohnson.bsky.social
Immunologist/cell biologist, also a fan of beautiful places. Warning: may post cat pics
Reposted by Louise Johnson
This #FluorescenceFriday we used our recombinant target binding reagent Ankyron® clone CE30111 (green) to detect Perlecan in the extracellular matrix deposited by HUVECs, with nuclei counterstained using DAPI (blue). Image captured using our brilliant @discoverecho.bsky.social #microscope.
October 17, 2025 at 7:40 AM
This #FluorescenceFriday we used our recombinant target binding reagent Ankyron® clone CE30111 (green) to detect Perlecan in the extracellular matrix deposited by HUVECs, with nuclei counterstained using DAPI (blue). Image captured using our brilliant @discoverecho.bsky.social #microscope.
Reposted by Louise Johnson
This #FluorescenceFriday we’re exploring microtubules using Ankyron® clone AP40945, which binds TBCA (red), with DAPI-stained nuclei (blue). Shown in HeLa cells. Image captured with our lovely @discoverecho.bsky.social #microscope. Learn more: proimmune.com/ankyron
September 12, 2025 at 7:47 AM
This #FluorescenceFriday we’re exploring microtubules using Ankyron® clone AP40945, which binds TBCA (red), with DAPI-stained nuclei (blue). Shown in HeLa cells. Image captured with our lovely @discoverecho.bsky.social #microscope. Learn more: proimmune.com/ankyron
Happy #Caturday! Princess Fifi Trixibelle Murder Mittens (first of her name) is enjoying the weekend #cats #adoptdontshop #pond
June 21, 2025 at 6:59 PM
Happy #Caturday! Princess Fifi Trixibelle Murder Mittens (first of her name) is enjoying the weekend #cats #adoptdontshop #pond
Reposted by Louise Johnson
Fascinating insights this #FluorescenceFriday, into complement delay-accelerating factor (DAF, CD55), identified by our Ankyron™ AC40301 (red) in HeLa cells (nuclei, blue) on @discoverecho.bsky.social #microscope.
Find out more about our #animal-free Ankyrons™ at www.proimmune.com/ankyron
Find out more about our #animal-free Ankyrons™ at www.proimmune.com/ankyron
May 2, 2025 at 7:22 AM
Fascinating insights this #FluorescenceFriday, into complement delay-accelerating factor (DAF, CD55), identified by our Ankyron™ AC40301 (red) in HeLa cells (nuclei, blue) on @discoverecho.bsky.social #microscope.
Find out more about our #animal-free Ankyrons™ at www.proimmune.com/ankyron
Find out more about our #animal-free Ankyrons™ at www.proimmune.com/ankyron
I wish “smell-o-vision” was a thing, as these #lilyofthevalley smell incredible. #Spring #April #gardenpond #nativeplant #uk
April 27, 2025 at 1:04 PM
I wish “smell-o-vision” was a thing, as these #lilyofthevalley smell incredible. #Spring #April #gardenpond #nativeplant #uk
Reposted by Louise Johnson
Exquisite filamentous patterns for #FluorescenceFriday thanks to Ankyron™ AU41488 specific to CD29 cell surface receptor β1 integrin (red) in a dendritic cell, with nucleus counterstained with DAPI (blue), on our lovely @discoverecho.bsky.social Echo Revolve #microscope.
#immunology #animalfree
#immunology #animalfree
April 25, 2025 at 7:12 AM
Exquisite filamentous patterns for #FluorescenceFriday thanks to Ankyron™ AU41488 specific to CD29 cell surface receptor β1 integrin (red) in a dendritic cell, with nucleus counterstained with DAPI (blue), on our lovely @discoverecho.bsky.social Echo Revolve #microscope.
#immunology #animalfree
#immunology #animalfree
Reposted by Louise Johnson
#MicroscopyMonday! IHC with an insulin-specific #animalfree Ankyron AB40258 (brown) clearly identifying β-cells in islets of Langerhans of human pancreas (nuclei in blue) using #Leica autostainer and @discoverecho.bsky.social #microscope in colour camera upright configuration. proimmune.com\ankyron
April 7, 2025 at 8:14 AM
#MicroscopyMonday! IHC with an insulin-specific #animalfree Ankyron AB40258 (brown) clearly identifying β-cells in islets of Langerhans of human pancreas (nuclei in blue) using #Leica autostainer and @discoverecho.bsky.social #microscope in colour camera upright configuration. proimmune.com\ankyron
Reposted by Louise Johnson
This #FluorescenceFriday we use an Ankyron™ to specifically label the Golgi apparatus marker RCAS1 (red), counterstaining actin cytoskeleton (green) and nucleus (blue) on @discoverecho.bsky.social microscope. Look out for our other organelle-specific #animalfree Ankyrons™ at proimmune.com\ankyron
April 4, 2025 at 7:17 AM
This #FluorescenceFriday we use an Ankyron™ to specifically label the Golgi apparatus marker RCAS1 (red), counterstaining actin cytoskeleton (green) and nucleus (blue) on @discoverecho.bsky.social microscope. Look out for our other organelle-specific #animalfree Ankyrons™ at proimmune.com\ankyron
I disturbed another occupant of the Oxford Science Park but they were illegally fishing - goldfish saved!
March 28, 2025 at 1:15 PM
I disturbed another occupant of the Oxford Science Park but they were illegally fishing - goldfish saved!
Reposted by Louise Johnson
Shining a light on Endoglin (aka CD105) for #FluorescenceFriday, shown in red using #animalfree Ankyron™ clone AY40419 (nuclei blue), highly expressed in vascular endothelial cells like these HUVEC with crucial roles in angiogenesis & TGFβ signaling. On our @discoverecho.bsky.social #microscope
March 28, 2025 at 8:09 AM
Shining a light on Endoglin (aka CD105) for #FluorescenceFriday, shown in red using #animalfree Ankyron™ clone AY40419 (nuclei blue), highly expressed in vascular endothelial cells like these HUVEC with crucial roles in angiogenesis & TGFβ signaling. On our @discoverecho.bsky.social #microscope
Reposted by Louise Johnson
This dendritic cell is ready for #FluorescenceFriday! Here, we used our interferon-α8-specific #animalfree Ankyron™ ANK01178_5_AW40550 (red) and nuclei counterstained with DAPI (blue) on @discoverecho.bsky.social #microscope
Find out more:
www.proimmune.com/ankyron
#cytokine
@afruk.bsky.social
Find out more:
www.proimmune.com/ankyron
#cytokine
@afruk.bsky.social
March 21, 2025 at 8:40 AM
This dendritic cell is ready for #FluorescenceFriday! Here, we used our interferon-α8-specific #animalfree Ankyron™ ANK01178_5_AW40550 (red) and nuclei counterstained with DAPI (blue) on @discoverecho.bsky.social #microscope
Find out more:
www.proimmune.com/ankyron
#cytokine
@afruk.bsky.social
Find out more:
www.proimmune.com/ankyron
#cytokine
@afruk.bsky.social
Reposted by Louise Johnson
#FluorescenceFriday insight into Lupus La protein, highly distinctive cytoplasmic staining (red) revealed by our #animalfree Ankyron™ - nuclei counterstained with DAPI (blue) on @discoverecho.bsky.social #microscope
#bioscience #immunology #animalfreeresearch #cytoplasm
@afruk.bsky.social
#bioscience #immunology #animalfreeresearch #cytoplasm
@afruk.bsky.social
March 14, 2025 at 8:03 AM
#FluorescenceFriday insight into Lupus La protein, highly distinctive cytoplasmic staining (red) revealed by our #animalfree Ankyron™ - nuclei counterstained with DAPI (blue) on @discoverecho.bsky.social #microscope
#bioscience #immunology #animalfreeresearch #cytoplasm
@afruk.bsky.social
#bioscience #immunology #animalfreeresearch #cytoplasm
@afruk.bsky.social
Reposted by Louise Johnson
Delighted that we contributed to exciting work from Zuben Sauna’s lab (FDA) using Ankyrons™ specific to 3 #COVID19 spike protein variants, which neutralized all 3 in a pseudovirus-based neutralization assay.
doi.org/10.1208/s122...
#BLI #immunology #animalfree
doi.org/10.1208/s122...
#BLI #immunology #animalfree
March 13, 2025 at 3:23 PM
Delighted that we contributed to exciting work from Zuben Sauna’s lab (FDA) using Ankyrons™ specific to 3 #COVID19 spike protein variants, which neutralized all 3 in a pseudovirus-based neutralization assay.
doi.org/10.1208/s122...
#BLI #immunology #animalfree
doi.org/10.1208/s122...
#BLI #immunology #animalfree
Reposted by Louise Johnson
🚨 Last chance to register for BSI Oxford and @immunologyoxford.bsky.social's Oxford Immunology Symposium! #OIS25
📅 Date: 24 March 2025
📍 Location: Oxford Mathematical Institute
🎤 Featuring talks on adaptive & innate immunity and interdisciplinary insights
Register NOW: t.co/tlXp9dddqo
📅 Date: 24 March 2025
📍 Location: Oxford Mathematical Institute
🎤 Featuring talks on adaptive & innate immunity and interdisciplinary insights
Register NOW: t.co/tlXp9dddqo
March 11, 2025 at 11:10 AM
🚨 Last chance to register for BSI Oxford and @immunologyoxford.bsky.social's Oxford Immunology Symposium! #OIS25
📅 Date: 24 March 2025
📍 Location: Oxford Mathematical Institute
🎤 Featuring talks on adaptive & innate immunity and interdisciplinary insights
Register NOW: t.co/tlXp9dddqo
📅 Date: 24 March 2025
📍 Location: Oxford Mathematical Institute
🎤 Featuring talks on adaptive & innate immunity and interdisciplinary insights
Register NOW: t.co/tlXp9dddqo
Reposted by Louise Johnson
It's #FluorescenceFriday! Here's a lovely clear image using #animalfree Ankyron™ specific to cell cycle protein Cyclin A1 (red), with F-actin counterstained by phalloidin (green) on @discoverecho.bsky.social #microscope Find out more www.proimmune.com/ankyron #immunology #3Rs
@afruk.bsky.social
@afruk.bsky.social
March 7, 2025 at 8:02 AM
It's #FluorescenceFriday! Here's a lovely clear image using #animalfree Ankyron™ specific to cell cycle protein Cyclin A1 (red), with F-actin counterstained by phalloidin (green) on @discoverecho.bsky.social #microscope Find out more www.proimmune.com/ankyron #immunology #3Rs
@afruk.bsky.social
@afruk.bsky.social
Reposted by Louise Johnson
Is #WesternBlotWednesday a thing?! Why not celebrate those perfect western blots? Here's our #animalfree Ankyron™ specifically binding human β-actin (37.3 kDa), both recombinant protein and in cell line lysates. Perfect loading control for westerns!
#3Rs #westernblot
@afruk.bsky.social
#3Rs #westernblot
@afruk.bsky.social
March 5, 2025 at 9:10 AM
Is #WesternBlotWednesday a thing?! Why not celebrate those perfect western blots? Here's our #animalfree Ankyron™ specifically binding human β-actin (37.3 kDa), both recombinant protein and in cell line lysates. Perfect loading control for westerns!
#3Rs #westernblot
@afruk.bsky.social
#3Rs #westernblot
@afruk.bsky.social
I love being part of this project and product line. Also, continually impressed at the sharpness of the images we achieve on our (epifluorescence) Echo Revolve microscope through the digital haze reduction software
Fab fine filaments of the cytoskeleton for #FluorescenceFriday lit up by our #animalfree Ankyron™ to β-actin (red), with nuclei counterstained with DAPI (blue) on our Echo Revolve #microscope @discoverecho.bsky.social An alternative counterstain to phalloidin! #immunology #3Rs @afruk.bsky.social
February 28, 2025 at 11:53 AM
I love being part of this project and product line. Also, continually impressed at the sharpness of the images we achieve on our (epifluorescence) Echo Revolve microscope through the digital haze reduction software
Reposted by Louise Johnson
Spectacular cell structure #FluorescenceFriday, with an #animalfree Ankyron™ against Calreticulin to specifically label the endoplasmic reticulum (red) in HUVEC, counterstaining actin cytoskeleton (green) and nuclei (blue) on our Echo Revolve #microscope
#bioscience #immunology #3Rs
@AFR_UK
#bioscience #immunology #3Rs
@AFR_UK
February 21, 2025 at 7:20 AM
Spectacular cell structure #FluorescenceFriday, with an #animalfree Ankyron™ against Calreticulin to specifically label the endoplasmic reticulum (red) in HUVEC, counterstaining actin cytoskeleton (green) and nuclei (blue) on our Echo Revolve #microscope
#bioscience #immunology #3Rs
@AFR_UK
#bioscience #immunology #3Rs
@AFR_UK
For #WindowsWednesday a view of Stourhead #nationaltrust from a room not usually open to the public. The guides were amazing and really brought it to life
February 19, 2025 at 6:53 PM
For #WindowsWednesday a view of Stourhead #nationaltrust from a room not usually open to the public. The guides were amazing and really brought it to life
Reposted by Louise Johnson
Delicate red dendrites for #FluorescenceFriday - here our #animalfree Ankyron binds Tumor Necrosis Factor Receptor Superfamily member 1B (TNFR2, in red) - Nuclei counterstained with DAPI (blue) on @Discover_Echo microscope #immunology #3Rs #biomedical #research
@AFR_UK
@AFR_UK
February 14, 2025 at 8:13 AM
Delicate red dendrites for #FluorescenceFriday - here our #animalfree Ankyron binds Tumor Necrosis Factor Receptor Superfamily member 1B (TNFR2, in red) - Nuclei counterstained with DAPI (blue) on @Discover_Echo microscope #immunology #3Rs #biomedical #research
@AFR_UK
@AFR_UK
Not sure if it is a thing on this platform but happy #caturday to all who celebrate (also please check out the amaryllis that I have managed to not kill yet)
February 8, 2025 at 7:01 PM
Not sure if it is a thing on this platform but happy #caturday to all who celebrate (also please check out the amaryllis that I have managed to not kill yet)
Reposted by Louise Johnson
It's February #FluorescenceFriday! Here's a beautiful image of #animalfree Ankyron™ against proliferation marker Ki67 (red) in dividing HeLa cells counterstained with phalloidin (showing actin cytoskeleton in green)
@Discover_Echo
#immunology #cells #biomedical #3Rs
@AFR_UK
@Discover_Echo
#immunology #cells #biomedical #3Rs
@AFR_UK
February 7, 2025 at 8:22 AM
It's February #FluorescenceFriday! Here's a beautiful image of #animalfree Ankyron™ against proliferation marker Ki67 (red) in dividing HeLa cells counterstained with phalloidin (showing actin cytoskeleton in green)
@Discover_Echo
#immunology #cells #biomedical #3Rs
@AFR_UK
@Discover_Echo
#immunology #cells #biomedical #3Rs
@AFR_UK
Reposted by Louise Johnson
It's #FluorescenceFriday! We used our #animalfree antibody replacement Ankyron™ (only 15kDa) specifically binding CD31 (PECAM1) fluorescently tagged & small enough to get into tight junctions of endothelial cells using Echo Revolve #microscope @Discover_Echo #immunology #3Rs
January 31, 2025 at 8:46 AM
It's #FluorescenceFriday! We used our #animalfree antibody replacement Ankyron™ (only 15kDa) specifically binding CD31 (PECAM1) fluorescently tagged & small enough to get into tight junctions of endothelial cells using Echo Revolve #microscope @Discover_Echo #immunology #3Rs