A/Prof. Diana Stojanovski
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drdstojanovski.bsky.social
A/Prof. Diana Stojanovski
@drdstojanovski.bsky.social
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Mitochondrial Biologist, Science Enthusiast, Eternal Optimist.
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Reposted by A/Prof. Diana Stojanovski
plosbiology.org
#Legionella pumps 100s of effector proteins into host cells, including to #mitochondria. @drdstojanovski.bsky.social &co show that effector LpPIP is a tail-anchored protein of the MOM that recruits protein phosphatase 1 to modulate the host mitochondrial P-proteome @plosbiology.org 🧪 plos.io/4f2T5kE
Top: HeLa cells expressing FLAGLpPIP, or FLAGRVxFdead were stained with 100 nM MitoTracker Deep Red FM (mitochondria; MTDR) and, following fixation, processed for immunofluorescence using antibodies against FLAG (green) and PPP1CB (magenta). Nuclei were stained with Hoechst 33258 (blue). Scale bar represents 10 μm. Fluorescence signal profiles of FLAG and PPP1CB were measured across the indicated line of interest. Bottom: AlphaFold3 structure of LpPIP-PPP1CB complex labeled with the active site (purple) and three substrate binding grooves (yellow) of PPP1CB.
July 25, 2025 at 1:57 PM