Colibri Cytometry
banner
colibricytometry.bsky.social
Colibri Cytometry
@colibricytometry.bsky.social
Tips & tricks for flow cytometry
@labliston.bsky.social

More on the blog here:
https://www.colibri-cytometry.com/blog

Also on Substack:
https://substack.com/@olivertburton
Here's why I do not recommend running new samples using the same unmixing matrix across multiple days. Read more on www.colibri-cytometry.com/post/stabili...
May 13, 2025 at 1:51 PM
Tips today on how to achieve stable unmixing over time by using spectral reference libraries.
www.colibri-cytometry.com/post/stabili...
Stabilizing your unmixing
Today's post pulls from a lot of previous posts on this blog to show how to effectively eliminate batch effects in your flow from an experimental side. Stabilizing the unmixing is a big part of this.
www.colibri-cytometry.com
April 28, 2025 at 12:12 PM
Do you use phenograph to analyze your cytometry data? Read today's post to learn how to avoid potential pitfalls, particularly if you're using either of the implementations in R.
www.colibri-cytometry.com/post/the-pec...
The peculiarities of Phenograph
Phenograph is one of the more popular clustering algorithms for flow cytometry.
www.colibri-cytometry.com
April 14, 2025 at 11:53 AM
Today's tip: when running your single-color controls, take it slow. The tortoise, as it turns out, beats the hare for spectral flow cytometry.
www.colibri-cytometry.com/post/unmixin...
Unmixing: the tortoise beats the hare
Today's tip: when running your single-color controls, take it slow.
www.colibri-cytometry.com
March 31, 2025 at 1:34 PM
Today's post is very simple--we're just looking at how to create reusable settings on the Cytek Aurora.
www.colibri-cytometry.com/post/creatin...
March 29, 2025 at 10:24 AM