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claricehongky.bsky.social
@claricehongky.bsky.social
79 followers 200 following 3 posts
Postdoc in Anders Hansen lab @ MIT | PhD from Barak Cohen lab @ WUSTL | Gene regulation ❤️ | 🇸🇬
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claricehongky.bsky.social
Proud of my amazing friend for this super cool piece of work! ♥️
matteomazzocca.bsky.social matteomazzocca.bsky.social @matteomazzocca.bsky.social · May 14
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Super excited to share my first preprint from @andersshansen.bsky.social Lab! We used MINFLUX to track chromatin (H2B-Halo and Fbn2 locus) at an unprecedented 200 μs, then combined it with SPT to span μs-minutes (H2B) or SPT & Super-Res Live-Cell Imaging (SRLCI) to span μs-hours (Fbn2)
May 14, 2025 at 9:52 PM
claricehongky.bsky.social
Excited to share my first preprint from the @andersshansen.bsky.social lab! It's got genomics, AI, and biology - all wrapped up in a great paper. Also very grateful to my amazing collaborator, Fan, for all his hard work. Please check it out and let me know what you think!
tinyurl.com/bdfvtd6f
Genome structure mapping with high-resolution 3D genomics and deep learning
Gene expression is often regulated by distal enhancers through cell-type-specific 3D looping interactions, but comprehensive mapping of these interactions across cell types is experimentally intractab...
tinyurl.com
May 7, 2025 at 6:50 PM
claricehongky.bsky.social
So excited to see Yawei's manuscript on long-range MPRAs out! Some really great insights into distal enhancer regulation 🙂
genometdcc.org GenomeTDCC @genometdcc.org · Apr 23
🚨PRE-PRINT 🧪🧬🖥️👩‍🔬
Long-range massively parallel reporter assay reveals rules of distal enhancer-promoter interactions
From Barak Cohen's lab at @washu.bsky.social

Read the pre-print 👇
doi.org/10.1101/2025...
Learn more about the research from the Cohen lab: bclab.wustl.edu
From the BioRxiv pre-print article "Long-range massively parallel reporter assay reveals rules of distal enhancer-promoter interactions" by Yawei Wu et al. Fig. 1. Design of long-range MPRA. a, Design of the enhancer landing pad and the promoter landing pad. The enhancer landing pad contains a dual promoter driving expression of an mTagBFP fluorescent protein and a thymidine kinase (TK) gene for counterselection, flanked by FRT and F3 sites for RMCE. available under aCC-BY-NC 4.0 International license. was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made bioRxiv preprint doi: https://doi.org/10.1101/2025.04.21.649048; this version posted April 22, 2025. The copyright holder for this preprint (which The promoter landing pad expresses the mEmerald fluorescent protein and iCaspase9 for counterselection and is flanked by loxFAS and loxP sites for orthogonal RMCE. b-c, Workflow of long-range MPRA. eBC: enhancer barcode; rBC: random barcode; dBC: distance barcode. A barcoded enhancer library is integrated into pooled long-range landing pad cells through Flpase-facilitated recombination. After counterselection with Ganciclovir, cells with successful integration are enriched. Cells are sorted into different bins based on mScarlet fluorescence level. Genomic DNA is extracted from each bin; barcodes are amplified and sequenced. Reporter gene expression of each enhancer at each distance is calculated as the weighted average of fluorescence levels across bins.
April 23, 2025 at 9:10 PM