Bertrand Theulot
@bertrandtht.bsky.social
Postdoctoral associate, Smith Lab, NYU
New paper! Loss of the Rabl configuration in single-chromosome yeast yields only local replication changes, measured by nanopore sequencing with NanoForkSpeed and Nanotiming. academic.oup.com/nar/article/...
Replication program of a single-chromosome budding yeast strain
Abstract. Nuclear architecture and chromosome folding are often speculated to influence genome replication. In yeasts, centromeres cluster close to the spi
academic.oup.com
August 14, 2025 at 1:58 PM
New paper! Loss of the Rabl configuration in single-chromosome yeast yields only local replication changes, measured by nanopore sequencing with NanoForkSpeed and Nanotiming. academic.oup.com/nar/article/...
Reposted by Bertrand Theulot
I am so proud to share a major part of my graduate school research, published today in Science, where we use cell-free replication assays and cryo-EM to describe a central mechanism of eukaryotic DNA replication:
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
G-quadruplex–stalled eukaryotic replisome structure reveals helical inchworm DNA translocation
DNA G-quadruplexes (G4s) are non–B-form DNA secondary structures that threaten genome stability by impeding DNA replication. To elucidate how G4s induce replication fork arrest, we characterized fork ...
www.science.org
March 6, 2025 at 7:40 PM
I am so proud to share a major part of my graduate school research, published today in Science, where we use cell-free replication assays and cryo-EM to describe a central mechanism of eukaryotic DNA replication:
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
NanoTiming is now in Nature Communications! High-resolution DNA replication timing using nanopore sequencing shows that Rif1 regulates only specific telomeres: www.nature.com/articles/s41...
Telomere-to-telomere DNA replication timing profiling using single-molecule sequencing with Nanotiming - Nature Communications
Current temporal studies of DNA replication are either complex or low-resolution. Here, the authors introduce Nanotiming, a simple and inexpensive method producing high-resolution, telomere-to-telomer...
www.nature.com
January 3, 2025 at 8:22 AM
NanoTiming is now in Nature Communications! High-resolution DNA replication timing using nanopore sequencing shows that Rif1 regulates only specific telomeres: www.nature.com/articles/s41...