<span><b>Background and Clinical Significance</b>: Granulomatous reactions are rare but clinically significant complications of aesthetic procedures involving dermal fillers, particularly in individuals with underlying immune dysregulation. These reactions present diagnostic and therapeutic challenges, es</span> …

This study presents a platform for minimal-invasive manufacturing of synthetic polymer-based microimplants directly in alive organisms by one-photon photopolymerization and multi-photon 3D laser printing. Employing embryos of the teleost fish Oryzias latipes and fruit fly Drosophila melanogaster , it delineates how these entirely biocompatible microimplants show no significant immunogenicity and bear great potential to be specifically utilized for engineering tissue morphogenesis. Abstract Multi-photon 3D laser printing has gathered much attention in recent years as a means of manufacturing biocompatible scaffolds that can modify and guide cellular behavior in vitro. However, in vivo tissue engineering efforts have been limited so far to the implantation of beforehand 3D printed biocompatible scaffolds and in vivo bioprinting of tissue constructs from bioinks containing cells, biomolecules, and printable hydrogel formulations. Thus, a comprehensive 3D laser printing platform for in vivo and in situ manufacturing of microimplants raised from synthetic polymer-based inks is currently missing. Here, a platform for minimal-invasive manufacturing of microimplants directly in the organism is presented by one-photon photopolymerization and multi-photon 3D laser printing. Employing a commercially available elastomeric ink giving rise to biocompatible synthetic polymer-based microimplants, first applicational examples of biological responses to in situ printed microimplants are demonstrated in the teleost fish Oryzias latipes and in embryos of the fruit fly Drosophila melanogaster . This provides a framework for future studies addressing the suitability of inks for in vivo 3D manufacturing. The platform bears great potential for the direct engineering of the intricate microarchitectures in a variety of tissues in model organisms and beyond.

Multi-photon 3D laser printing has gathered much attention in recent years as a means of manufacturi

Multi-photon 3D laser printing has gathered much attention in recent years as a means of manufacturi