Interesting organoid work on an often overlooked lineage emerging from the Lateral Plate Mesoderm: ventral dermis!
#devbio #evodevo #MesodermIsStillCool 🧬🎈⚾
#devbio #evodevo #MesodermIsStillCool 🧬🎈⚾
The latest story from my group. Working toward build regionally distinct skin and amnion piece by piece.
Lateral plate mesoderm directs human amnion and ventral skin organoid formation https://www.biorxiv.org/content/10.1101/2025.11.05.685987v1
November 9, 2025 at 4:51 PM
Interesting organoid work on an often overlooked lineage emerging from the Lateral Plate Mesoderm: ventral dermis!
#devbio #evodevo #MesodermIsStillCool 🧬🎈⚾
#devbio #evodevo #MesodermIsStillCool 🧬🎈⚾
How do endodermal cells behave during gastrulation? In this paper, the authors found that a gradient of BMP regulates mesoderm and endoderm convergent extension. Endoderm cells also use the mesoderm and Cxcr4a signalling to guide their movement. The imaging is beautiful!
doi.org/10.1016/j.cd...
doi.org/10.1016/j.cd...
October 17, 2025 at 6:27 PM
How do endodermal cells behave during gastrulation? In this paper, the authors found that a gradient of BMP regulates mesoderm and endoderm convergent extension. Endoderm cells also use the mesoderm and Cxcr4a signalling to guide their movement. The imaging is beautiful!
doi.org/10.1016/j.cd...
doi.org/10.1016/j.cd...
6/ You can do so much with it! Here are some case studies:
Demo 1: stretch the endoderm (ventral epithelium of the developing amniote 🐣 embryo, here in 🟢) → observe mechanical coupling with the subjacent mesoderm (brightfield ⬜) 🎥
Demo 1: stretch the endoderm (ventral epithelium of the developing amniote 🐣 embryo, here in 🟢) → observe mechanical coupling with the subjacent mesoderm (brightfield ⬜) 🎥
October 12, 2025 at 12:51 PM
6/ You can do so much with it! Here are some case studies:
Demo 1: stretch the endoderm (ventral epithelium of the developing amniote 🐣 embryo, here in 🟢) → observe mechanical coupling with the subjacent mesoderm (brightfield ⬜) 🎥
Demo 1: stretch the endoderm (ventral epithelium of the developing amniote 🐣 embryo, here in 🟢) → observe mechanical coupling with the subjacent mesoderm (brightfield ⬜) 🎥
6/9 Single-cell transcriptomic and chromatin profiling of zebrafish tumors revealed that EWSR1::FLI1 expression in neural crest cells drives a mesenchymal/mesodermal shift, with mesodermal genes like #Brachyury (T) strongly upregulated. #NCCs #mesoderm #reprogramming
October 9, 2025 at 9:06 PM
6/9 Single-cell transcriptomic and chromatin profiling of zebrafish tumors revealed that EWSR1::FLI1 expression in neural crest cells drives a mesenchymal/mesodermal shift, with mesodermal genes like #Brachyury (T) strongly upregulated. #NCCs #mesoderm #reprogramming
Nice work from Val Wilson & co on neuromesodermal progenitors
SOX2/TBXT co-expressing cells are self-propagating bipotent NMPs
Increasing TBXT levels (not SOX2/TBXT ratio) switch NMPs from neural- to mesoderm-biased
www.biorxiv.org/content/10.1...
SOX2/TBXT co-expressing cells are self-propagating bipotent NMPs
Increasing TBXT levels (not SOX2/TBXT ratio) switch NMPs from neural- to mesoderm-biased
www.biorxiv.org/content/10.1...
Brachyury expression levels predict lineage potential and axis-forming ability of in vitro derived neuromesodermal progenitors
Neuromesodermal progenitors (NMPs) produce the spinal cord and musculoskeleton in the elongating anterior-posterior axis. In vivo, NMPs possess dual potency, coinciding with regions coexpressing SOX2 ...
www.biorxiv.org
September 29, 2025 at 8:33 AM
Nice work from Val Wilson & co on neuromesodermal progenitors
SOX2/TBXT co-expressing cells are self-propagating bipotent NMPs
Increasing TBXT levels (not SOX2/TBXT ratio) switch NMPs from neural- to mesoderm-biased
www.biorxiv.org/content/10.1...
SOX2/TBXT co-expressing cells are self-propagating bipotent NMPs
Increasing TBXT levels (not SOX2/TBXT ratio) switch NMPs from neural- to mesoderm-biased
www.biorxiv.org/content/10.1...
The church of the holy trinity: endoderm, mesoderm and ectoderm!
September 16, 2025 at 3:04 PM
The church of the holy trinity: endoderm, mesoderm and ectoderm!
Our work on the evolution of head mesoderm development in chordates is finally out in Nature Communications!
www.nature.com/articles/s41...
It includes a thorough analysis and annotation of the single-cell transcriptomic atlas of an amphioxus neurula.
www.nature.com/articles/s41...
It includes a thorough analysis and annotation of the single-cell transcriptomic atlas of an amphioxus neurula.
An amphioxus neurula stage cell atlas supports a complex scenario for the emergence of vertebrate head mesoderm - Nature Communications
The evolution of the vertebrate head mesoderm involved the emergence of new structures and cell types. Here the authors generated a cell atlas of the cephalochordate neurula to study the origins of th...
www.nature.com
May 29, 2024 at 7:12 AM
Our work on the evolution of head mesoderm development in chordates is finally out in Nature Communications!
www.nature.com/articles/s41...
It includes a thorough analysis and annotation of the single-cell transcriptomic atlas of an amphioxus neurula.
www.nature.com/articles/s41...
It includes a thorough analysis and annotation of the single-cell transcriptomic atlas of an amphioxus neurula.
Origin of Ewing sarcoma by embryonic reprogramming of neural crest to mesoderm https://www.biorxiv.org/content/10.1101/2024.10.27.620438v1
Origin of Ewing sarcoma by embryonic reprogramming of neural crest to mesoderm https://www.biorxiv.org/content/10.1101/2024.10.27.620438v1
Ewing sarcoma is a malignant small round blue cell tumor of bones and soft tissues caused by chromos
www.biorxiv.org
October 30, 2024 at 12:50 AM
Origin of Ewing sarcoma by embryonic reprogramming of neural crest to mesoderm https://www.biorxiv.org/content/10.1101/2024.10.27.620438v1
We hematologists are taught that all blood starts from gastrulation. When we precisely followed these steps that mimic gastrulation, hematoendothelial mesoderm, and hematopoiesis, it took 2 weeks. But some red cell precursors appear much faster, like a few days… 2/
November 8, 2025 at 10:06 AM
We hematologists are taught that all blood starts from gastrulation. When we precisely followed these steps that mimic gastrulation, hematoendothelial mesoderm, and hematopoiesis, it took 2 weeks. But some red cell precursors appear much faster, like a few days… 2/
my sexually undifferentiated intermediate mesoderm drove the ladies *wild* let me tell you
January 21, 2025 at 7:19 PM
my sexually undifferentiated intermediate mesoderm drove the ladies *wild* let me tell you
Finally, the excellent postdoc work of Emmanuel Haillot is out, where he dissected the integration of Wnt, MAPK and Notch signaling in defining mesoderm and endoderm identities in the diploblast Nematostella. Strikingly similar to sea urchins. www.nature.com/articles/s41...
August 29, 2025 at 9:04 PM
Finally, the excellent postdoc work of Emmanuel Haillot is out, where he dissected the integration of Wnt, MAPK and Notch signaling in defining mesoderm and endoderm identities in the diploblast Nematostella. Strikingly similar to sea urchins. www.nature.com/articles/s41...
Jiang, J. and Aegerter, C. M. (2023). An integrated vertex model of the mesoderm invagination during the embryonic development of #Drosophila. J Theor Biol 572: 111581. www.sciencedirect.com/science/arti...
December 4, 2023 at 3:55 PM
Jiang, J. and Aegerter, C. M. (2023). An integrated vertex model of the mesoderm invagination during the embryonic development of #Drosophila. J Theor Biol 572: 111581. www.sciencedirect.com/science/arti...
Substrate Rigidity Modulates Segmentation Clock Dynamics in Isolated Presomitic Mesoderm Cells https://www.biorxiv.org/content/10.1101/2024.07.02.601712v1
Substrate Rigidity Modulates Segmentation Clock Dynamics in Isolated Presomitic Mesoderm Cells https://www.biorxiv.org/content/10.1101/2024.07.02.601712v1
The segmentation clock, a genetic oscillator in the presomitic mesoderm (PSM), is known to be influe
www.biorxiv.org
July 5, 2024 at 12:33 AM
Substrate Rigidity Modulates Segmentation Clock Dynamics in Isolated Presomitic Mesoderm Cells https://www.biorxiv.org/content/10.1101/2024.07.02.601712v1
Together these results show Bik1 encodes a Gag protein essential for mesoderm development. But how can a Gag protein encoded by a recent invader play such an important in a developmental process deeply conserved in vertebrates? WTF is goin on?!
a white cat is sitting on a box and says someone tell me what is happening
ALT: a white cat is sitting on a box and says someone tell me what is happening
media.tenor.com
April 30, 2025 at 10:45 AM
Together these results show Bik1 encodes a Gag protein essential for mesoderm development. But how can a Gag protein encoded by a recent invader play such an important in a developmental process deeply conserved in vertebrates? WTF is goin on?!
One cell became a few thousand, and along the way changes occurred. The invisible lines in your skin are the evidence of those changes.
Pigmentation in zebras and tigers follow different patterns, but using visible pigments.
Pigmentation in zebras and tigers follow different patterns, but using visible pigments.
September 21, 2023 at 1:44 AM
One cell became a few thousand, and along the way changes occurred. The invisible lines in your skin are the evidence of those changes.
Pigmentation in zebras and tigers follow different patterns, but using visible pigments.
Pigmentation in zebras and tigers follow different patterns, but using visible pigments.
Our screen revealed several novel regulators of germ layer differentiation, including a strong paraxial mesoderm-specific role of the CCR4-NOT component Cnot8. Loss of Cnot8 resulted in gastruloid patterning defects such as the formation of multiple Brachyury poles and aberrant growth.
August 6, 2025 at 4:14 PM
Our screen revealed several novel regulators of germ layer differentiation, including a strong paraxial mesoderm-specific role of the CCR4-NOT component Cnot8. Loss of Cnot8 resulted in gastruloid patterning defects such as the formation of multiple Brachyury poles and aberrant growth.
Thank you, 😊 not so color-blind friendly but, this was live zebrafish mesoderm explants with membrane and nuclei markers and green is microtubules (SiR-Tubulin).
December 1, 2024 at 1:03 AM
Thank you, 😊 not so color-blind friendly but, this was live zebrafish mesoderm explants with membrane and nuclei markers and green is microtubules (SiR-Tubulin).
Tbx1 stabilizes differentiation of the cardiopharyngeal mesoderm and drives morphogenesis in the pharyngeal apparatus https://www.biorxiv.org/content/10.1101/2024.07.16.603669v1
Tbx1 stabilizes differentiation of the cardiopharyngeal mesoderm and drives morphogenesis in the pharyngeal apparatus https://www.biorxiv.org/content/10.1101/2024.07.16.603669v1
Background: TBX1 is required for the development of the pharyngeal apparatus. In the mouse, fish, an
www.biorxiv.org
July 19, 2024 at 3:31 PM
Tbx1 stabilizes differentiation of the cardiopharyngeal mesoderm and drives morphogenesis in the pharyngeal apparatus https://www.biorxiv.org/content/10.1101/2024.07.16.603669v1
Our findings not only point to #paraxial mesoderm as a likely new source of embryonic haematopoietic cells, but may also explain the bone marow phenotype sometimes observed in paediatric patients with alveolar #rhabdomyosarcoma
December 4, 2024 at 2:26 PM
Our findings not only point to #paraxial mesoderm as a likely new source of embryonic haematopoietic cells, but may also explain the bone marow phenotype sometimes observed in paediatric patients with alveolar #rhabdomyosarcoma
Creating an asymmetric heart: @tbonnely.bsky.social @audreydsg.bsky.social @meilhacsigolene.bsky.social &co identify Notch3 as a novel asymmetric factor in lateral plate mesoderm that acts as a genetic modifier of NODAL to regulate heart morphogenesis @plosbiology.org 🧪 plos.io/42q6H4L
April 1, 2025 at 4:48 PM
Creating an asymmetric heart: @tbonnely.bsky.social @audreydsg.bsky.social @meilhacsigolene.bsky.social &co identify Notch3 as a novel asymmetric factor in lateral plate mesoderm that acts as a genetic modifier of NODAL to regulate heart morphogenesis @plosbiology.org 🧪 plos.io/42q6H4L
Gonadal sex determination in vertebrates: rethinking established mechanisms
In this Review, dagmar-wilhelm.bsky.social, Marie-Christine Chaboissier & colleagues discuss mechanisms and genes currently known to be involved in sex determination and differentiation:
doi.org/10.1242/dev....
In this Review, dagmar-wilhelm.bsky.social, Marie-Christine Chaboissier & colleagues discuss mechanisms and genes currently known to be involved in sex determination and differentiation:
doi.org/10.1242/dev....
![Figure 2 Formation of the adrenal and gonadal primordia in vertebrates. (A) The adrenogonadal primordium [AGP, indicated in colour with the future adrenal primordium (AP) in pink and the future gonadal primordium (GP) in yellow] arises from lateral plate mesoderm at 9.5 days post-coitum (dpc) in mouse embryos, at HH13 (Hamburger Hamilton stage) in chicken embryos and at Carnegie stage (CS) 14 in human embryos. (B) The spatial and temporal appearances of the AP and GP vary between species, with the AP and GP being spatially, temporally and phenotypically distinct: the AP develops earlier and is clearly separated from the GP in human (left). In mouse (centre), the AGP splits into a more cranial AP, and a more caudal GP at 10.5 dpc, and in chicken (right), the AP and GP are derived from two adjacent layers of coelomic epithelium and/or the mesonephric mesenchyme. MD, mesonephric duct; MT, mesonephric tubules.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:btitwyhiegkb6f62oguxt6m6/bafkreiefglvka37f7mfotv6y6mtycbnemz2symhtmg4xhofmdnyy2lfl3m@jpeg)
April 2, 2025 at 8:51 AM
Gonadal sex determination in vertebrates: rethinking established mechanisms
In this Review, dagmar-wilhelm.bsky.social, Marie-Christine Chaboissier & colleagues discuss mechanisms and genes currently known to be involved in sex determination and differentiation:
doi.org/10.1242/dev....
In this Review, dagmar-wilhelm.bsky.social, Marie-Christine Chaboissier & colleagues discuss mechanisms and genes currently known to be involved in sex determination and differentiation:
doi.org/10.1242/dev....
Happy to share with you some beautiful expression patterns of amphioxus orthologues of genes expressed in vertebrate lateral plate and pharyngeal mesoderm. Congrats Anael. www.sciencedirect.com/science/arti...
Expression analysis of amphioxus orthologues of genes expressed in vertebrate lateral plate or pharyngeal mesoderm
The lateral plate mesoderm of vertebrates, which borders the other mesodermal territories, develops during embryogenesis into a variety of tissues and…
www.sciencedirect.com
June 18, 2025 at 7:49 AM
Happy to share with you some beautiful expression patterns of amphioxus orthologues of genes expressed in vertebrate lateral plate and pharyngeal mesoderm. Congrats Anael. www.sciencedirect.com/science/arti...
IF they had known the first word was gonna be all caps, they could have rearranged it to be SEGMENTATION instead of ... MUCH!!
November 26, 2024 at 7:11 PM
IF they had known the first word was gonna be all caps, they could have rearranged it to be SEGMENTATION instead of ... MUCH!!
CDX2 dose-dependently influences the gene regulatory network underlying human extraembryonic mesoderm development https://www.biorxiv.org/content/10.1101/2024.01.25.577277v1
CDX2 dose-dependently influences the gene regulatory network underlying human extraembryonic mesoderm development https://www.biorxiv.org/content/10.1101/2024.01.25.577277v1
Proper regulation of gene dosage is critical for the development of the early embryo and the extraem
www.biorxiv.org
January 26, 2024 at 5:36 PM
CDX2 dose-dependently influences the gene regulatory network underlying human extraembryonic mesoderm development https://www.biorxiv.org/content/10.1101/2024.01.25.577277v1
Finally, our proposed model showing LEC differentiation through in situ specification from ETV2+PROX1+ paraxial mesoderm-derived lymphangioblasts
January 13, 2025 at 2:31 PM
Finally, our proposed model showing LEC differentiation through in situ specification from ETV2+PROX1+ paraxial mesoderm-derived lymphangioblasts