By tracking rare cycling hiPSC-derived cardiomyocytes, we can monitor cell proliferation (useful in regeneration) and multinucleation and endoreplication (useful for assessing maturation) as well as structure and function integration #CCAwarePhenotyping #CCAwareRegenerativeStudies #MethodInACellLine

New light-sheet capture of 4-color hiPSC-cardioids reveals compaction before calcium transients initiation. 90 % exit cycle by d4.5 → maturation on cue. #CCAwarePhenotyping #MethodInACellLine

Phase-locked motility
Each nucleus gets a %-cell-cycle score that rides with its mask and trajectory. Compare shape or speed by cycle progress, not frame time. Watch HaCaTs sprint through physical space and cell cycle (CC) phases! #PhaseLockedMotility #CCAwarePhenotyping