#ASBMB2023 Adam Perriman-modifying proteins with amphiphilic corona 🌞. Tissue engineering. Self-growing ECM using ➕️/surfactant coated thrombin. 🦷-on-a-chip. Smart gels, substrate🔁matrix feedback, porous matrices, cells immobilised, living flow reactors. 🪱 printing?! 😎🤯
November 21, 2024 at 4:16 PM
#ASBMB2023 Adam Perriman-modifying proteins with amphiphilic corona 🌞. Tissue engineering. Self-growing ECM using ➕️/surfactant coated thrombin. 🦷-on-a-chip. Smart gels, substrate🔁matrix feedback, porous matrices, cells immobilised, living flow reactors. 🪱 printing?! 😎🤯
Jyoti Gurung- talking torque, LOV, sadness, new LOV, iLOV and swimming in the light. Engineering (messing with) bacterial motors to respond to light. 🚨💡See you on the bus! #ASBMB2023
November 21, 2024 at 4:30 PM
Jyoti Gurung- talking torque, LOV, sadness, new LOV, iLOV and swimming in the light. Engineering (messing with) bacterial motors to respond to light. 🚨💡See you on the bus! #ASBMB2023
Shimadzu🎖@GrasLab story of tenacity, bouncing back, making moves and approaching your scientific 'crush'. Find your champions, ditch the detractors. 🤩 HLA 🌭; T-cells love 🌭😄: characterising peptide presentation in viral variants. HLA-B15 🛡 lucky ducks!! #ASBMB2023 👏👏👏
November 21, 2024 at 4:09 PM
Shimadzu🎖@GrasLab story of tenacity, bouncing back, making moves and approaching your scientific 'crush'. Find your champions, ditch the detractors. 🤩 HLA 🌭; T-cells love 🌭😄: characterising peptide presentation in viral variants. HLA-B15 🛡 lucky ducks!! #ASBMB2023 👏👏👏
Phoebe Chen at #ASBMB2023 coined the term 'bioinformatics'!? Computer Scientist - machine learning - useful patterns - medical images - exon discovery - and a rockstar 👩🎤 *is that Bowie? I hope so*
November 21, 2024 at 4:43 PM
Phoebe Chen at #ASBMB2023 coined the term 'bioinformatics'!? Computer Scientist - machine learning - useful patterns - medical images - exon discovery - and a rockstar 👩🎤 *is that Bowie? I hope so*
Shogo Nakano! #ASBMB2023 Protein conjugation by design with S. avermitilis ancestral sortases - can conjugate primary amines, can synthesise modified antibody; and sortagged enzyme immobilisation to polyaminized beads. High recyclability, high activity!
November 21, 2024 at 4:09 PM
Shogo Nakano! #ASBMB2023 Protein conjugation by design with S. avermitilis ancestral sortases - can conjugate primary amines, can synthesise modified antibody; and sortagged enzyme immobilisation to polyaminized beads. High recyclability, high activity!
Stefan Bröer on developing amino acid transporters the right way 😄 Targeting specific transporters with nM affinity and characterisation with cryoTEM. Docking limited help! Real binding site defines an allosteric inhibitor mechanism👍 #ASBMB2023
November 21, 2024 at 4:30 PM
Stefan Bröer on developing amino acid transporters the right way 😄 Targeting specific transporters with nM affinity and characterisation with cryoTEM. Docking limited help! Real binding site defines an allosteric inhibitor mechanism👍 #ASBMB2023
Ranji Meemanage-Cebreco *COI declared* Leveraging SHKs and MCPs as biosensors. Chimeras, fluorescent screening and BAM 💥 functionality, many constit active... negative screening module *chair is standing*🫨 Chimeric sensor library screening feasible! Awesome 😊😊 #ASBMB2023
November 21, 2024 at 4:09 PM
Ranji Meemanage-Cebreco *COI declared* Leveraging SHKs and MCPs as biosensors. Chimeras, fluorescent screening and BAM 💥 functionality, many constit active... negative screening module *chair is standing*🫨 Chimeric sensor library screening feasible! Awesome 😊😊 #ASBMB2023
Qian Guo rounding out the session with Retromer - a central regulator for endosomal trafficking. WASH complex associated with Retromer. Another alphafold classic! 🧶 Disordered aDLF and LFa repeats. All bind FIRM domain of Snx27. Even a 💎🎣 video 😁. Cute credits ❤️ #ASBMB2023
November 21, 2024 at 4:16 PM
Qian Guo rounding out the session with Retromer - a central regulator for endosomal trafficking. WASH complex associated with Retromer. Another alphafold classic! 🧶 Disordered aDLF and LFa repeats. All bind FIRM domain of Snx27. Even a 💎🎣 video 😁. Cute credits ❤️ #ASBMB2023
@JoelPMackay with a dynamic talk incorporating some beautiful single molecule expts with @van__Oijen and a marvellous tangly dreads metaphore. RIP Flojo 💔 #ASBMB2023
November 21, 2024 at 4:43 PM
@JoelPMackay with a dynamic talk incorporating some beautiful single molecule expts with @van__Oijen and a marvellous tangly dreads metaphore. RIP Flojo 💔 #ASBMB2023
Richard Payne: RaPID mRNA display. A trillion! mRNAs/cyclic peptides. Amplify protein binders to select for high affinity. CoV2 antivirals post-lockdown spike:ACE2 PPI inhibitor. pM affinities, inadvertent diagnostics? #ASBMB2023
November 21, 2024 at 4:37 PM
Richard Payne: RaPID mRNA display. A trillion! mRNAs/cyclic peptides. Amplify protein binders to select for high affinity. CoV2 antivirals post-lockdown spike:ACE2 PPI inhibitor. pM affinities, inadvertent diagnostics? #ASBMB2023
Brian Morris #ASBMB2023 Proteomics of resilience to mortality mediated by FOXO3 longevity genotype. Implications for serum proteomics for personalised medicine? Great example of longitudinal cohort study.
November 21, 2024 at 4:43 PM
Brian Morris #ASBMB2023 Proteomics of resilience to mortality mediated by FOXO3 longevity genotype. Implications for serum proteomics for personalised medicine? Great example of longitudinal cohort study.
A blurry photo of Irina @voineagulab - on enhancers in brain-derived astrocytes. Identified genes regulated by enhancers are overrepresented in Alzheimer's disease. Incredible CRISPRi screening 🤯. #ASBMB2023
November 21, 2024 at 4:43 PM
A blurry photo of Irina @voineagulab - on enhancers in brain-derived astrocytes. Identified genes regulated by enhancers are overrepresented in Alzheimer's disease. Incredible CRISPRi screening 🤯. #ASBMB2023
Ciara Wallis - (quietly) acid trips! Back to coupled lactate/proton transport. MD 😊 Lactate binding to His and a flapping Phe. Unfavourable for lactate to leave. Lactate protonation by His donor to evict lactate. With Kasimir Gregory. Proton hopping! #ASBMB2023
November 21, 2024 at 4:23 PM
Ciara Wallis - (quietly) acid trips! Back to coupled lactate/proton transport. MD 😊 Lactate binding to His and a flapping Phe. Unfavourable for lactate to leave. Lactate protonation by His donor to evict lactate. With Kasimir Gregory. Proton hopping! #ASBMB2023
Josh Hardy on viral SPA and tomography. I've been sworn to secrecy, suffice to say it is an edifying talk beautifully presented. #ASBMB2023
November 21, 2024 at 4:16 PM
Josh Hardy on viral SPA and tomography. I've been sworn to secrecy, suffice to say it is an edifying talk beautifully presented. #ASBMB2023
@renaemryan1 Glutamate/Cl transporter. Crosslinking, cryoTEM, and a cool Arg/His swap with pH dependent switch from Cl to Na? transport (!) at the pinch point 🤏. Amazing 👏 #ASBMB2023
November 21, 2024 at 4:30 PM
@renaemryan1 Glutamate/Cl transporter. Crosslinking, cryoTEM, and a cool Arg/His swap with pH dependent switch from Cl to Na? transport (!) at the pinch point 🤏. Amazing 👏 #ASBMB2023
Sanjib Senapati on ZIKV E protein shell - why is viral particle thermally stable relative to Dengue? MD coarsegr simulations replicate experimental observations. All atom sim 12m atoms-Interface contacts same or better with higher temp! Mech: Polar and electrostatic. #ASBMB2023
November 21, 2024 at 4:37 PM
Sanjib Senapati on ZIKV E protein shell - why is viral particle thermally stable relative to Dengue? MD coarsegr simulations replicate experimental observations. All atom sim 12m atoms-Interface contacts same or better with higher temp! Mech: Polar and electrostatic. #ASBMB2023
Here at #ASBMB2023 in the dying days of Twitter. So enjoyed @JohnKuriyan 's talk - kicking us off in style. Lovely walk in past some brutalist architecture on my first visit to ANU.
November 21, 2024 at 4:50 PM
Here at #ASBMB2023 in the dying days of Twitter. So enjoyed @JohnKuriyan 's talk - kicking us off in style. Lovely walk in past some brutalist architecture on my first visit to ANU.
Structural Biol ctd - Rosemary Cater: Flvxr1 choline transporter - mouse models, function, structure - in PM on lumenal side of membrane. KO mouse 👇 choline uptake. pH dependence RECONSTUTITED PROTEOLIPOSOMES! SPA + FABs 2.5 A!!! In and out + choline bound. Amazing. #ASBMB2023
November 21, 2024 at 4:23 PM
Structural Biol ctd - Rosemary Cater: Flvxr1 choline transporter - mouse models, function, structure - in PM on lumenal side of membrane. KO mouse 👇 choline uptake. pH dependence RECONSTUTITED PROTEOLIPOSOMES! SPA + FABs 2.5 A!!! In and out + choline bound. Amazing. #ASBMB2023
Bostjan Kobe up first in the Structural Biology session. MicroED; XFEL; SPA - All of the methodologies. SARM1 as a transformer. Some cool 2D classes! TIR domains as cooperative assemblies- adaptors, receptors and enzymes. #intimidating utility #ASBMB2023
November 21, 2024 at 4:23 PM
Bostjan Kobe up first in the Structural Biology session. MicroED; XFEL; SPA - All of the methodologies. SARM1 as a transformer. Some cool 2D classes! TIR domains as cooperative assemblies- adaptors, receptors and enzymes. #intimidating utility #ASBMB2023
@BruningLab up next with a shoutout to @FrkicRebecca - PPRgamma - ligand binding to inhibit phosphorylation as a diabetes therapeutic? Unique structure trapped with H12 in inhibited position 👌 #ASBMB2023
November 21, 2024 at 4:23 PM
@BruningLab up next with a shoutout to @FrkicRebecca - PPRgamma - ligand binding to inhibit phosphorylation as a diabetes therapeutic? Unique structure trapped with H12 in inhibited position 👌 #ASBMB2023
@PaolaLaurino originally an organic chemist 😯 Indels- part 1) loopless, loopfull and remote - Chitinase: acquisition of antifungal activity. Phylogeny of loopiness, plus mutation to access new fungi type. Another barnstorming talk at #ASBMB2023
November 21, 2024 at 4:37 PM
@PaolaLaurino originally an organic chemist 😯 Indels- part 1) loopless, loopfull and remote - Chitinase: acquisition of antifungal activity. Phylogeny of loopiness, plus mutation to access new fungi type. Another barnstorming talk at #ASBMB2023
Sonia Henriques at #ASBMB2023 inhibiting PPIs of LDH5 with spider beta hairpins; gets into the cytoplasm!! ; but acute toxicity due to membrane disruption. Engineered to eliminate membrane disruption. Still inhibit PPI, still get into cells, and kill cancer cells. Amazing 👏 🕷❤️
November 21, 2024 at 4:37 PM
Sonia Henriques at #ASBMB2023 inhibiting PPIs of LDH5 with spider beta hairpins; gets into the cytoplasm!! ; but acute toxicity due to membrane disruption. Engineered to eliminate membrane disruption. Still inhibit PPI, still get into cells, and kill cancer cells. Amazing 👏 🕷❤️
Aidan Grosas up next in the best session - Membrane Transporter Biology - MDR P. aeruginosa/Drug efflux. Efflux inhibitors? IM/OM transporter; flexible entrance to a vestibule with a hydrophobic pit. Functional rotation mechanism debunked? 2A cryo! #ASBMB2023
November 21, 2024 at 4:30 PM
Aidan Grosas up next in the best session - Membrane Transporter Biology - MDR P. aeruginosa/Drug efflux. Efflux inhibitors? IM/OM transporter; flexible entrance to a vestibule with a hydrophobic pit. Functional rotation mechanism debunked? 2A cryo! #ASBMB2023
@Dr_EmmaWatson on anticoagulant proteins from blood feeders 🫣. Yay! Gross. Heavy post-translational modifications - enter: solid phase synthesis with non native or PTMd amino acids. Native chemical ligation, aqueous conditions, no protecting groups 🤘. PRE-CLINICAL!! #ASBMB2023
November 21, 2024 at 4:30 PM
@Dr_EmmaWatson on anticoagulant proteins from blood feeders 🫣. Yay! Gross. Heavy post-translational modifications - enter: solid phase synthesis with non native or PTMd amino acids. Native chemical ligation, aqueous conditions, no protecting groups 🤘. PRE-CLINICAL!! #ASBMB2023
Elaine Tao - beautiful talk - PIP modulation of voltage gated sodium channels. Atomistic MD simulations of NAV1.7 - PIP binding stabilises the inactive state. Resting state model: CTD restricts PIP binding. #ASBMB2023 A master-class in clear scicomms. Thank you Elaine! 😊
November 21, 2024 at 4:37 PM
Elaine Tao - beautiful talk - PIP modulation of voltage gated sodium channels. Atomistic MD simulations of NAV1.7 - PIP binding stabilises the inactive state. Resting state model: CTD restricts PIP binding. #ASBMB2023 A master-class in clear scicomms. Thank you Elaine! 😊