zack chiang
@zchiang.bsky.social
microscopy x epigenomics | buenrostro lab | harvard/broad
To show the power of this approach, we applied ExIGS to progeria cells with nuclear lamina abnormalities
By combining expansion and 3D genome sequencing in the same nucleus, we can literally see how the abnormal lamin topology changes the structure of chromosomes
6/
By combining expansion and 3D genome sequencing in the same nucleus, we can literally see how the abnormal lamin topology changes the structure of chromosomes
6/
November 11, 2024 at 7:33 PM
To show the power of this approach, we applied ExIGS to progeria cells with nuclear lamina abnormalities
By combining expansion and 3D genome sequencing in the same nucleus, we can literally see how the abnormal lamin topology changes the structure of chromosomes
6/
By combining expansion and 3D genome sequencing in the same nucleus, we can literally see how the abnormal lamin topology changes the structure of chromosomes
6/
In situ sequencing measures the 3D location and genomic position of each DNA fragment, letting us trace the path of every chromosome in the nucleus
We also do expansion IF imaging to measure which fragments co-localize with protein landmarks
5/
We also do expansion IF imaging to measure which fragments co-localize with protein landmarks
5/
November 11, 2024 at 7:33 PM
In situ sequencing measures the 3D location and genomic position of each DNA fragment, letting us trace the path of every chromosome in the nucleus
We also do expansion IF imaging to measure which fragments co-localize with protein landmarks
5/
We also do expansion IF imaging to measure which fragments co-localize with protein landmarks
5/
Expanding the genome evenly is hard because DNA is a polymer, so here we use the Buenrostro lab's favorite enzyme Tn5 to make fragments beforehand
We then do Illumina sequencing, but instead of on a flowcell, all enzymatics are performed ~inside~ the expanded nucleus
4/
We then do Illumina sequencing, but instead of on a flowcell, all enzymatics are performed ~inside~ the expanded nucleus
4/
November 11, 2024 at 7:33 PM
Expanding the genome evenly is hard because DNA is a polymer, so here we use the Buenrostro lab's favorite enzyme Tn5 to make fragments beforehand
We then do Illumina sequencing, but instead of on a flowcell, all enzymatics are performed ~inside~ the expanded nucleus
4/
We then do Illumina sequencing, but instead of on a flowcell, all enzymatics are performed ~inside~ the expanded nucleus
4/
Fortunately, we had Fei Chen & Ed Boyden, the inventors of expansion microscopy on board!
In ExM, samples are physically enlarged in gels, allowing superresolution imaging without fancy microscopes
3/
In ExM, samples are physically enlarged in gels, allowing superresolution imaging without fancy microscopes
3/
November 11, 2024 at 7:33 PM
Fortunately, we had Fei Chen & Ed Boyden, the inventors of expansion microscopy on board!
In ExM, samples are physically enlarged in gels, allowing superresolution imaging without fancy microscopes
3/
In ExM, samples are physically enlarged in gels, allowing superresolution imaging without fancy microscopes
3/
7 years ago, I met a junior fellow named Jason Buenrostro who blew me away with a vision of futuristic genomic technologies
Today, we (Ajay Labade, Caroline Comenho) are excited to share our first steps into that future: Expansion in situ genome sequencing
1/
Today, we (Ajay Labade, Caroline Comenho) are excited to share our first steps into that future: Expansion in situ genome sequencing
1/
November 11, 2024 at 7:33 PM
7 years ago, I met a junior fellow named Jason Buenrostro who blew me away with a vision of futuristic genomic technologies
Today, we (Ajay Labade, Caroline Comenho) are excited to share our first steps into that future: Expansion in situ genome sequencing
1/
Today, we (Ajay Labade, Caroline Comenho) are excited to share our first steps into that future: Expansion in situ genome sequencing
1/