CR also takes a swing at cellular senescence! With IRISeq, We observed a significant reduction in senescence-associated gene expression across various brain regions and cell types upon CR.

Intriguingly, CR’s boost to neurogenesis is time-sensitive! 🕰️ It helps preserve neurogenic cell populations (stem cells & neuroblasts) earlier in life (e.g., at 12 months), delaying their depletion. However, this effect wasn't sustained at much later time points (18 & 24 months).

CR significantly counteracts the age-related expansion of disease-associated glial cells. We observed a striking reduction in inflammatory cell states like disease-associated microglia & oligodendrocytes – pointing to CR’s power to dampen brain inflammation.

The precise cellular & regional mechanisms of CR's benefits to the aging brain have been elusive. To bridge this gap, we brought cutting-edge tech – highly scalable single-nuc(EasySci) & spatial transcriptomics (IRISeq, kudos to Abdul & Weirong) – to dissect this age-old yet resilient strategy.