8/ In this way, we can map out the full surface. We show that the surface functionalization is patchy and heterogeneous between particles. This will allow studying the surface functionalization at the single-particle level to design and produce the nanoparticles of the future.

4/ The PSF doesn’t always deform in the same way, but the PSF shape depends on the fluorophore’s position relative to the nanoparticle. This means that the PSF shape encodes information about the fluorophore’s position, which we can use to our advantage during localization.

PSFs are just Gaussians, right??

Not when your fluorophore💡is close to a nanoparticle 🟠!
Excited to share our recent ACS Nano paper 🥳 in which we present a novel PSF-fitting approach for localization microscopy near nanoparticles.

pubs.acs.org/doi/full/10....

Main findings below in🧵[1/10]

4/ The PSF doesn’t always deform in the same manner, but the PSF shape depends on the fluorophore’s position relative to the nanoparticle. This means that the PSF shape encodes information about the fluorophore’s position, which we can use to our advantage during localization.

10/ 🛜 The #inTRACKtive platform is open source (github.com/royerlab/inT...) and has multiple use cases: (i) to explore the Virtual Embryo Zoo, (ii) to visualize your own cell tracking data, 2D or 3D, and (iii) to host your own customizable instance of the viewer.

9/ 🔗 With #inTRACKtive, your exact viewing configuration - cells, zoom level, selections, and more - can be shared via a simple hyperlink. Collaboration has never been easier! 👩‍🔬

8/ 📱 #inTRACKtive works in any browser, including on tablets and smartphones, offering flexibility in how users interact with the data. A larger screen and keyboard are recommended to utilize the full UI functionalities.

6/ 🏆 Within the Virtual Embryo Zoo, users can visualize the tracked datasets with #inTRACKtive, download the complete tracking data (ready to visualize in #napari) and see details of the datasets.